Pattern of serum protein fractions in dairy cows during different stages of gestation and lactation

Abstract

In dairy cows the period of transition from late gestation to early lactation is recognized as inducing considerable metabolic adaptation. The aim of this study was to analyse modifications in serum protein values occurring during the dry and the transition period and during lactation in a group of five Holstein cows of high average milk production. For all subjects, selected on the basis of their pregnancy status, blood samples were collected at different physiological phases: dry period (-60, -30 d to calving), transition period (almost 7 d to calving, 7 d after calving), and lactation (weeks 2, 5 and 15 after calving), for a total of eight blood samples for each cow. On each blood sample total proteins and electrophoresis analysis were performed. On the data obtained, normally distributed (P<0·05, Kolmogorov-Smirnov's test), one-way Repeated Measure Analysis of Variance (ANOVA), was applied to evaluate the influence of different stages of gestation and lactation on the considered parameters. Results showed a significant effect on total proteins, α1-globulins, β-globulins, γ-globulins and albumin/globulin ratio. Most of the detected modifications were related to the transition from gestation to lactation, indicating that it is a period of great metabolic stress for cows. On the basis of the obtained results we can affirm that the pattern of serum protein fraction rn could give information about dehydration, plasma volume expansion and hepatic function occurring during the peripartum period in dairy cows.

Clinical, Immunological and Epidemiological Studies of Nasopharyngeal Myiasis in Camels slaughtered in Al-Muthanna Province

“…The results of our study indicate that levels of α1-globulins, α2-globulins, β-globulins and γ-globulins were statistically significant increase in camels suffering from parasitic infection comprised to healthy. The results were in agreement with many studies (Alberghina et al, 2010, Piccione et al, 2011Tóthová et al, 2013). Serum protein electrophoresis standard method for fractionation and quantification of serum proteins is electrophoresis in clinical biochemistry.…”

Plasma estrone sulfate, clinical biochemistry, and milk yield of dairy cows carrying a fetus from a bull or its clone

“…Serum protein profile did not reveal differences between fetus' sire or by CBW class; this implies that g-globulin were unaffected, and therefore the immune system for the passive transfer of immunity was not compromised with CLONE progeny or with increased CBW. The absolute values of a 1 -globulin in this article are slightly lesser than those reported by Piccione et al [30]; this fraction is generally associated to a group of acute-phase proteins that are signal of inflammatory conditions [31]. In the light of our results, also bglobulin did not differ between the 2 groups of recipients.…”

Serum proteins and their diagnostic utility in veterinary medicine: a review

“…Grunberg et al (2011) observed lower concentrations of total serum proteins in cows around parturition than outside the parturient period and in the following phases of lactation, which may be associated with the transfer of immunoglobulins from the bloodstream to the mammary gland for the synthesis of colostrum (Roubies et al 2006). Piccione et al (2011;Piccione et al 2012b) found increasing values of α-globulins in dairy cows and ewes post-partum, which may be related to the higher concentrations of the acute phase proteins in response to the processes occurring around the time of parturition.…”