Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Gole, Anand; Dixit, Vaishali; Lala, Neeta L.; Sainkar, S. R.; Pant, Aditi; Sastry, Murali

doi:10.1016/s0927-7765(02)00005-x

Cited by 9 publications

(9 citation statements)

References 17 publications

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“…Yarrowia lipolytica is one of the most studied non‐conventional yeasts for use in enzyme production as well as a host for genetic engineering purposes 2–4. It is a lipase‐producing strictly aerobic micro‐organism and it has been observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme‐based biotransformations 5. Nevertheless under certain situations Y. lipolytica may not be able to cope with competition from other types of micro‐organisms present simultaneously in the natural reaction substrate 6.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of organosilicon thin films for selective adhesion of Yarrowia lipolytica yeast cells

Lehocký¹,

Amaral²,

Šťahel³

et al. 2007

J of Chemical Tech & Biotech

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The adhesion of Yarrowia lipolytica and Saccharomyces cerevisiae on organosilicon thin films deposited on polycarbonate substrates was investigated through a series of adhesion tests in order to obtain a selective substrate for Y. lipolytica cell adhesion. Organosilicon thin films were prepared using atmospheric pressure surface barrier discharge. The surface was characterized by its total surface energy with its components calculated using the acid-base theory. Assessment of adhesion via cell surface coverage was obtained by standard tests and also through image analysis of yeast cells on the organosilicon layers. The results show that it is possible to create organosilicon thin films for a selective adhesion of Y. lipolytica face to S. cerevisiae behaviour, for potential use in fixed bed biofilm reactors for industrial purposes involving enzyme-based biotransformations.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of organosilicon thin films for selective adhesion of Yarrowia lipolytica yeast cells

Lehocký¹,

Amaral²,

Šťahel³

et al. 2007

J of Chemical Tech & Biotech

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“…These SEM images show that under these conditions, a negligibly small percentage of the yeast cells are bound to the surface of the membrane. It is well‐known that these yeast cells bind to hydrophobic regions of a surface (22, 23), and thus, the use of octadecylamine‐modified organic‐gold nanoparticle membranes provides an optimum and versatile surface for immobilization of the C. bombicola cells. It is known that the surface roughness effects can dramatically increase the hydrophobicity (36).…”

Section: Resultsmentioning

confidence: 99%

“…In this laboratory, we have been interested in assembly of specific cells on surfaces from the point of view of using the cells as sources of enzymes for biotransformations and synthesis of new materials. Some of us have recently shown that Yarrowia lipolitica (22) and Candida bombicola yeast cells (23) can be immobilized on patterned hydrophobic regions of thermally evaporated fatty lipid films. In the latter case, the enzyme cytochrome P450 present in the yeast cells was used to catalyze in situ ω and ω‐1 hydroxylation of arachidonic acid (AA).…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Candida bombicola Cells on Free-Standing Organic-Gold Nanoparticle Membranes and Their Use as Enzyme Sources in Biotransformations

Phadtare¹,

Shah²,

Prabhune³

et al. 2004

Biotechnol. Prog.

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Preparation of chemically functionalized biocompatible surfaces is of current interest, with application in the immobilization of various bioactive species such as DNA, enzymes, whole cells, etc. We report herein the one-step synthesis of a self-supporting gold nanoparticle membrane, its surface modification, and application in the immobilization of Candida bombicola (yeast) cells. The gold nanoparticle membrane is prepared by the spontaneous reduction of aqueous chloroaurate ions by a diamine at a liquid-liquid interface. The gold nanoparticles in the polymeric membrane may be capped with octadecylamine (ODA) molecules, thereby rendering the nanoparticle membrane hydrophobic. Exposure of the hydrophobized organic-gold nanoparticle membrane to C. bombicola yeast cells results in their binding to the membrane, possibly through nonspecific interactions such as hydrophobic interactions between the yeast cell walls and the ODA molecules. The enzyme cytochrome P450 present in the yeast cells immobilized on the organic-gold nanoparticle membrane was then used in the transformation of the arachidonic acid (AA) to sophorolipids followed by acid hydrolysis to form 20-hydroxyeicosatetraneoic acid (20-HETE). The organic-gold nanoparticle membrane-C. bombicola bioconjugate could be easily separated from the reaction medium and reused a number of times.

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“…Occasionally, the enzymes of interest may be unstable outside the cellular environment, and in such cases, immobilization of the cells would be important to catalyze reactions that are dependent on the unstable enzymes. Developing significantly on our earlier work where we demonstrated that Yarrowia lipolitica cells may be immobilized on fatty lipid films (16), we report herein the assembly of Candida bombicola yeast cells onto patterned thermally evaporated fatty amine thin films (octadecylamine, ODA) and the use of the enzyme cytochrome P450 present in the yeast cells to catalyze in situ the ω‐hydroxylation of arachidonic acid (AA) to 20‐hydroxyeicosatetraenoic acid (20‐HETE) (17, 18) (see Figure 1). Cytochrome P450 is a membrane protein and is known to be highly unstable outside the cells (18).…”

Section: Introductionmentioning

confidence: 99%

CandidabombicolaCells Immobilized on Patterned Lipid Films as Enzyme Sources for the Transformation of Arachidonic Acid to 20‐HETE

Phadtare

Parekh

Shah

et al. 2003

Biotechnology Progress

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Preparation of biocompatible surfaces for immobilization of enzymes and whole cells is an important aspect of biotechnology due to their potential applications in biocatalysis, biosensing, and immunological applications. In this report, patterned thermally evaporated octadecylamine (ODA) films are used for the immobilization of Candida bombicola cells. The attachment of the cells to the ODA film surface occurs possibly through nonspecific interactions such as hydrophobic interactions between the cell walls and the ODA molecules. The enzyme cytochrome P450 present in the immobilized yeast cells on the ODA film surface was used for the transformation of the arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE). The assembly of cells on the hydrophobic ODA surface was confirmed by quartz crystal microgravimetry (QCM), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). SEM images confirmed the strong binding of the yeast cells to the ODA film surface after biocatalytic reactions. Moreover, the biocomposite films could be easily separated from the reaction medium and reused.

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Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Cited by 9 publications

References 17 publications

Preparation and characterization of organosilicon thin films for selective adhesion of Yarrowia lipolytica yeast cells

Preparation and characterization of organosilicon thin films for selective adhesion of Yarrowia lipolytica yeast cells

Immobilization of Candida bombicola Cells on Free-Standing Organic-Gold Nanoparticle Membranes and Their Use as Enzyme Sources in Biotransformations

CandidabombicolaCells Immobilized on Patterned Lipid Films as Enzyme Sources for the Transformation of Arachidonic Acid to 20‐HETE

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