Patterned Photostimulation in the Brain

Anselmi, Francesca; Banerjee, Arkarup; Albeanu, Dinu F.

doi:10.1007/978-3-319-12913-6_9

Cited by 3 publications

(1 citation statement)

References 109 publications

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“…However, neurons are often activated simultaneously either by flashes of light that activate the whole population synchronously or by a light whose intensity is temporally modulated over the whole field of view (Boyden et al ., 2005). Yet, several methods exist to modulate the light spatially and have been used to uncage glutamate (Nawrot et al ., 2009) or activate channelrhodopsin (ChR2) expressing neurons (Guo et al ., 2009) (for review of available methods to stimulate neurons with both spatial and temporal resolution see Anselmi et al ., 2015). …”

Section: Introductionmentioning

confidence: 99%

Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control

Barral¹,

Reyes²

2017

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We studied a network of cortical neurons in culture and developed an innovative optical device to stimulate optogenetically a large neuronal population with both spatial and temporal precision. We first describe how to culture primary neurons expressing channelrhodopsin. We then detail the optogenetic setup based on the workings of a fast Digital Light Processing (DLP) projector. The setup is able to stimulate tens to hundreds neurons with independent trains of light pulses that evoked action potentials with high temporal resolution. During photostimulation, network activity was monitored using patch-clamp recordings of up to 4 neurons. The experiment is ideally suited to study recurrent network dynamics or biological processes such as plasticity or homeostasis in a network of neurons when a sub-population is activated by distinct stimuli whose characteristics (correlation, rate, and, size) were finely controlled.

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