Patterns of Genetic Variation within and among Gypsy Moth, Lymantria dispar (Lepidoptera: Lymantriidae), Populations

Harrison, Richard G.; Wintermeyer, Stephen F.; Odell, Thomas M.

doi:10.1093/aesa/76.4.652

Cited by 43 publications

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“…Homogeneity within the North American population has also been seen using isozyme and mtDNA RFLP analyses (Harrison et al, 1983). Approximately as much variation exists within the Asian population (0.26% distance) as is seen between the Asians and North Americans (0.27% distance).…”

Section: Discussionmentioning

confidence: 88%

“…The highest levels of morphological and developmental variation have been described in the far eastern regions of the range, especially Japan (Goldschmidt, 1940;Harrison et al, 1983), and it is possible that the species arose there and spread westward. Similar patterns of variation have been found j.Jsing isozyme (Harrison et al, 1983) and mitochondrial restriction site polymorphism (Bogdanowicz et al, 1993) analyses. An extensive study by Goldschmidt (1940) showed that some Asian/European crosses resulted in partial or full sex reversals, although Clarke & Ford (1980) were unable to reproduce these findings to the same extent.…”

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confidence: 99%

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Identification and characterization of a RAPD‐PCR marker for distinguishing Asian and North American gypsy moths

Garner

Slavicek

1996

Insect Molecular Biology

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The recent introduction of the Asian gypsy moth (Lymantria dispar L.) into North America has necessitated the development of genetic markers to distinguish Asian moths from the established North American population, which originated in Europe. We used RAPD‐PCR to identify a DNA length polymorphism that is diagnostic for the two moth strains. The polymorphism maps to an autosomal locus with codo‐minant Mendelian inheritance. DNA sequence analyses of the Asian and North American forms enabled development of locus‐specific primers so that this marker, designated FS‐1, will be useful for strain identification under varying conditions in different laboratories.

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Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

Identification and characterization of a RAPD‐PCR marker for distinguishing Asian and North American gypsy moths

Garner

Slavicek

1996

Insect Molecular Biology

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“…Theoretical studies indicate that a significant loss of genetic variation can occur after a founder event if the bottleneck persists for many generations (Maruyama & Fuerst 1985). Empirical studies also implicate founding events and population bottlenecks in the loss of protein variability (Harrison et al 1983). It is difficult to invoke these explanations here, because the history of the Hessian fly's invasion is not well documented, and we have not examined protein variability in possible source populations.…”

Section: Resultsmentioning

confidence: 99%

Electrophoretic Monomorphism in Six Biotypes and Two Populations of the Hessian Fly (Diptera: Cecidomyiidae)

Wellso¹,

Howard²,

Adams³

et al. 1988

Annals of the Entomological Society of America

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“…Between AGM and EGM, 14 SNPs were observed indicating clear divergence. Two haplotypes were observed in populations from Japan, but the divergence could not be defined further or Japanese gypsy moths [3,16]. Pfeifer et al [5]employed a specific primer ITS22 of nDNA that revealed fragment length polymorphisms among geographical populations of gypsy moths.…”

Section: Coi Gene Amplification Sequence Characteristics and Geograpmentioning

confidence: 99%

COI gene geographic variation of Gypsy moth (Lepidoptera: Lymantriidae) and a TaqMan PCR diagnostic assay

Lu¹,

An²,

Song³

et al. 2014

DNA Barcodes

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Gypsy moth, an important forest/urban pest worldwide, is separated into the European and Asian subspecies, and has important quarantine significance. Diagnostic technique that can accurately and quickly distinguish subspecies is lacking. This study aimed to evaluate genetic difference between the subspecies, and subsequently to develop a reliable and high throughput molecular based diagnostic tool for distinguishing the subspecies. COI genes of 25 gypsy moth samples from China, Russia, Mongolia, Japan and the United States were sequenced. DNASTAR analysis revealed that gypsy moth COI gene was 1531bp long. The UPGM phylogenetic tree constructed based on the COI gene indicated that European subspecies (U.S. population) and Asian subspecies were distinctively divided into two branches. Japanese populations had a far distantly relationship with other Asian populations forming a separate branch. There was a single base substitution (base transformation only) at 14 consistent locations between Asian and American populations, but 13 of them coded the same amino acid. A MGB proper and TaqMan assay was designed base on the base substitution at 406th bp that coded a different amino acid. This allele typing assay took only 4 hours and could accurately distinguish gypsy moth subspecies of Europe and Asia. The study enriches the knowledge basis of genetic differentiation of gypsy moth subspecies. And more importantly the TaqMan assay is the first report of such diagnostic tool that could deliver rapid and accurate results and suitable for routine quarantine inspections to distinguish Asian and European gypsy moth subspecies. This study was supported by the Ministry of Science and Technology of the People’s Republic of China (Science and technology supporting project: 2012BAK11B03; International cooperation project: 2009DFA31950) and Jiangsu Entry and Exit Inspection and Quarantine Bureau (2014KJ45).

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Patterns of Genetic Variation within and among Gypsy Moth, Lymantria dispar (Lepidoptera: Lymantriidae), Populations

Cited by 43 publications

References 0 publications

Identification and characterization of a RAPD‐PCR marker for distinguishing Asian and North American gypsy moths

Identification and characterization of a RAPD‐PCR marker for distinguishing Asian and North American gypsy moths

Electrophoretic Monomorphism in Six Biotypes and Two Populations of the Hessian Fly (Diptera: Cecidomyiidae)

COI gene geographic variation of Gypsy moth (Lepidoptera: Lymantriidae) and a TaqMan PCR diagnostic assay

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