Patterns of heavy and light chain utilization in the antibody response to single-stranded bacterial DNA in normal human subjects and patients with systemic lupus erythematosus

Robertson, Cheryl R.; Gilkeson, Gary S.; Ward, Michael M.; Pisetsky, David S.

doi:10.1016/0090-1229(92)90019-k

Cited by 29 publications

(16 citation statements)

References 43 publications

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“…This pattern contrasts SLE anti-DNA, which display an IgG1 predominance with both bacterial and mammalian [9]. To determine whether an IgG2 predominance is characteristic of the response to all bacterial DNA, anti-DNA assays were performed with subclassspecific reagents in the ELISA.…”

Section: Resultsmentioning

confidence: 98%

“…These antibodies bind with high selectivity to their respective DNA antigens and do not cross-react with other DNA. NHS antibodies to MC DNA are predominately IgG2k, suggesting induction by a T cell-independent mechanism that resembles the response to a bacterial carbohydrate [8,9]. Immunization experiments in mice fully confirm the ability of bacterial DNA to elicit specific antibody production [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

Drayton

Pisetsky

1997

Clinical and Experimental Immunology

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SUMMARYTo elucidate the mechanisms of anti-DNA production, we assessed the binding of sera of normal human subjects (NHS) and patients with SLE to a panel of bacterial and mammalian DNA. Using singlestranded DNA as antigens in an ELISA, NHS showed significant binding to some but not all bacterial DNA, while lacking reactivity to calf thymus DNA. Among bacterial DNA, the highest levels of binding were observed with DNA from Micrococcus lysodeikticus and Staphylococcus aureus. In contrast, SLE sera showed high levels of binding to all DNA tested. To evaluate further immunochemical properties of the anti-DNA antibodies, the subclass distribution of these responses was evaluated by subclass-specific reagents. While NHS showed a predominance of IgG2 antibodies to bacterial DNA, SLE sera had a predominance of IgG1 antibodies to these antigens. Together, these results provide further evidence for the antigenicity of bacterial DNA and suggest that NHS and SLE anti-DNA differ in the patterns of epitope recognition as well as mechanisms of induction.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

Drayton

Pisetsky

1997

Clinical and Experimental Immunology

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“…This has not been studied in MCTD. In addition, antibodies i against bacterial DNA are different in terms of heavy and light chain utilization in patients with SLE compared to normal individuals (21,22). This lends support to the suggestion that the anti-DNA antibodies in SLE patients may be a cross reaction, originally directed against an other antigen in a subject prone to polyclonal activation of the B cell system (20,23).…”

Section: Discussionmentioning

confidence: 52%

“…In animal immunization experi ments, DNA is poorly immunogenic; single-stranded DNA (ssDNA) elicits only a low titer antibody response, while double-stranded DNA (dsDNA) is inactive (19). When the reacitivity of ssDNA of various mammalian and bacterial species origin was tested, patients with SLE demonstrated serum antibodies to all six types of DNA tested, whereas normal persons showed appreciable an tibody response only to DNA obtained from certain bac teria (20,21). This implies that the response in patients with SLE is less specific than in healthy individuals.…”

Section: Discussionmentioning

confidence: 99%

“…The total im munoglobulin k/A, ratio is altered in some autoimmune diseases (5-8,15), one of them being SLE with raised k/X ratio of total serum immunoglobulins (5), The antibod ies to DNA from the bacterium Micrococcus lysodeikth cus synthesized in normal subjects were primarily of the k type (21). In contrast, antibodies to this bacterial DNA from SLE patients had more equivalent expression of k and A, chains (21). This indicates different immunologi cal processes in the reaction of SLE patients.…”

Section: Discussionmentioning

confidence: 99%

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Altered immunoglobulin concentrations and light chain ratios in juvenile onset mixed connective tissue disease

et al. 1995

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Immunological disturbances may result in altered immunoglobulin concentrations and kappa/lainbda light chain (k/X) ratios. We measured the k/X ratios of total serum immunoglobulins and of polyclonal IgG, A, and M separately as well as concentrations of these immunoglobulins in fourteen patients with juve nile onset mixed connective tissue disease. When comparing the patient group with a reference group the mean serum IgG and IgA concentrations were respectively 2.98 G/L (p=0.0012) and 0.79 G/L (p=0.0114) higher in the group of patients with juvenile onset mixed connective tissue disease. The mean IgM concentration was 0,39 G/L (p = 0.0002) lower. The mean k/X ratios of total serum immunoglobulins, serum IgG, and serum IgA were respectively 0.20 (p=0.0226), 0.28 (p~0.0016) and 0.10 (p=0.0732), higher in the group of patients with mixed connective tissue disease as compared with the reference group. Mean serum IgM k/X ratio, however, was 0.21 (p = 0.0046) lower. The alterations of the serum immunoglobulin concentrations and of the k/X ratios reflect immunological disturbances in patients with juvenile onset mixed connec tive tissue disease. The increased concentration of serum IgG and raised IgG k/X ratio and decreased concentration of serum IgM with decreased IgM K/X ratio in dicate that the synthesis of K-bearing immunoglobulins mainly is affected.

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The Role of CpG Sequences in the Induction of Anti-DNA Antibodies

Pisetsky

Wenk

Reich

2001

Clinical Immunology

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Patterns of heavy and light chain utilization in the antibody response to single-stranded bacterial DNA in normal human subjects and patients with systemic lupus erythematosus

Cited by 29 publications

References 43 publications

Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

Altered immunoglobulin concentrations and light chain ratios in juvenile onset mixed connective tissue disease

The Role of CpG Sequences in the Induction of Anti-DNA Antibodies

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