Patterns of ligninolytic enzymes in Trametes versicolor . Distribution of extra- and intracellular enzyme activities during cultivation on glucose, wheat straw and beech wood

Schlößer, Dietmar; Grey, Roland; Fritsche, W.

doi:10.1007/s002530050949

Cited by 115 publications

(80 citation statements)

References 27 publications

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“…Glucose exhaustion was also observed during the growth of Botryosphaeria sp. and Trametes versicolor when initial glucose concentrations of 5 and 10 g L -1 were used (6,26). Regardless of the culture condition, rapid acidification of the culture medium was observed within the first days of growth of T. villosa, with the pH reaching a value close to 3.5 at 7 days (Fig.…”

Section: Resultsmentioning

confidence: 97%

Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions

Yamanaka¹,

Soares²,

Matheus³

et al. 2008

Braz. J. Microbiol.

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The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first days of growth. Laccase was the main activity detected under the different conditions and was produced throughout the culture period of the fungus, irrespective of the growth phase. Supplementation of the medium with vegetable oil emulsified with a surfactant induced manganese-dependent peroxidase activity in T. villosa. Higher specific yields of laccase activity were obtained with the addition of copper.

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Section: Resultsmentioning

confidence: 97%

Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions

Yamanaka¹,

Soares²,

Matheus³

et al. 2008

Braz. J. Microbiol.

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“…Many white-rot fungi produce multiple laccase isoforms under the appropriate inductive conditions [5,6]. Most fungal laccases studied are extracellular proteins, but intracellular laccases have been detected in some fungi [7].…”

Section: +mentioning

confidence: 99%

Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation

Стоилова¹,

Krastanov²,

Stanchev³

2010

ABB

114

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A low-cost process for the production of laccases is necessary for a sustainable enzymatic wastewater treatment. Therefore, it is necessary to establish an easy and low-cost procedure for the production of laccase. In the present study the properties of crude laccase from Trametes versicolor produced by solidsubstrate fermentation is investigated. The application of the enzyme for dye decolorization is also studied. Crude laccase from the studied culture established maximal activity at 45ºC. The enzyme retained over 90% of its activity in the temperature range 40-47ºC and pH 4.5. The kinetic constants of the crude enzyme was also determined. In the presence of KCl, NaCl, CaCl 2 , MnSO 4 and MgSO 4 , laccase demonstrated high stability-over 50% of its initial activity was still retained after 4-month incubation.

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Section: Degradation Of T-np and 4-n-np By Fungal Culturesmentioning

confidence: 99%

“…The fungal dry weights were not affected by the addition of these compounds (data not shown). Manganese peroxidase, manganese-independent peroxidase (peroxidase, EC 1.11.1.7) and lignin peroxidase (EC 1.11.1.14) activities were assessed as previously described (Höfer & Schlosser, 1999;Schlosser et al, 1997) but were not observed during any of the experiments.Crude culture preparations derived from cultures amended with CuSO 4 and vanillic acid were concentrated, and samples from concentrates (corresponding to laccase activities of 150 and 350 mU for UHH 1-6-18-4 and C. aquatica, respectively) were subjected to SDS-PAGE under non-denaturing and non-reducing conditions. After activity staining with ABTS, two bands appeared for UHH 1-6-18-4, whereas the sample from C. aquatica revealed one major activity band and two less prominent bands appearing at nearly equal intensities (Fig.…”

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confidence: 99%

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

et al. 2005

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Degradation of technical nonylphenol (t-NP), known as an endocrine-disrupting compound mixture, was assessed, using the mitosporic fungal strain UHH 1-6-18-4 isolated from nonylphenol-contaminated river water, and a strain of the aquatic hyphomycete Clavariopsis aquatica. GC-MS analysis could resolve 12 peaks attributable to nonyl chain-branched t-NP isomers. All were degraded, to individual extents. Analysis of degradation metabolites suggested intracellular hydroxylation of the nonyl moieties of individual t-NP isomers. Further metabolites also indicated shortening of branched nonyl chains, and 4-hydroxybenzoic acid was identified as a t-NP breakdown product in UHH 1-6-18-4. The t-NP degradation efficiency was higher in UHH 1-6-18-4 than in C. aquatica, and a lower specificity in degradation of individual t-NP constituents in UHH 1-6-18-4 than in C. aquatica was observed. Strain UHH 1-6-18-4 concomitantly produced extracellular laccase under degradation conditions. A mixture of CuSO 4 and vanillic acid considerably enhanced laccase production in both fungi. Laccase preparations derived from UHH 1-6-18-4 and C. aquatica cultures also converted t-NP. Laccase-catalysed transformation of t-NP led to the formation of products with higher molecular masses than that of the parent compound. These results emphasize a role of fungi occurring in aquatic ecosystems in degradation of water contaminants with endocrine activity, which has not previously been considered. Furthermore, the results are in support of two different mechanisms employed by fungi isolated from aquatic environments to initiate t-NP degradation: hydroxylation of individual t-NP isomers at their branched nonyl chains and further breakdown of the alkyl chains of certain isomers; and attack of t-NP by extracellular laccase, the latter leading to oxidative coupling of primary radical products to compounds with higher molecular masses. INTRODUCTIONNonylphenols have increasingly gained attention because of their potential to mimic the action of natural hormones in vertebrates (Ying et al., 2002). They result from incomplete biodegradation of nonylphenol polyethoxylates (NPEOs), which have been widely used as non-ionic surfactants in industrial processes and households (Braun et al., 2003;Ying et al., 2002). Both nonylphenols and NPEOs are discharged into the environment, mainly due to incomplete removal in wastewater treatment facilities (Ying et al., 2002). Nonylphenols are more resistant to biodegradation than their parent compounds and hence are found worldwide in wastewater treatment plant effluents and rivers in concentrations of up to the mg l 21 range (Heemken et al., 2001;Kolpin et al., 2002;Stachel et al., 2003;Ying et al., 2002). Due to their hydrophobicity, they tend to adsorb onto surface water particles and sediments and accumulate in aquatic organisms (Heemken et al., 2001;Ying et al., 2002). Consequently, nonylphenols represent a serious environmental and human health risk. The assessment of biodegradative processes affecting the environmental...

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Patterns of ligninolytic enzymes in Trametes versicolor . Distribution of extra- and intracellular enzyme activities during cultivation on glucose, wheat straw and beech wood

Cited by 115 publications

References 27 publications

Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions

Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions

Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

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