Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis

González, José M.; Carlton, Bruce C.

doi:10.1016/s0147-619x(80)90038-4

Cited by 121 publications

(58 citation statements)

References 13 publications

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“…While cry1 encodes for proteins forming bipyramidal crystals and are related to toxicity to Lepidoptera [29] cry2 encodes for cuboidal proteins, toxic to Lepidoptera and Diptera [39]. Our molecular and electron microscopy analyses of Bt RT are in agreement with all this evidence, since this highly pathogenic strain has both genes ( Table 1) and both kinds of proteins ( Figure 8A).…”

Section: Molecular Characterization Of B Thuringiensis Strains and Csupporting

confidence: 83%

“…Generally, B. thuringiensis insecticidal protein toxin genes (cry) reside on large selftransmissible plasmids, and individual B. thuringiensis strains can harbor a diverse range of plasmids that can vary in number from 1 to 17 and in size from 2 to 80 MDa [29,30], although it has also been suggested that they are present in the chromosome [31]. In this context, to study the plasmid profiles of Bt strains is an important parameter to determine their identity, since the number and size of these is associated with a particular Bt strain.…”

Section: Molecular Characterization Of B Thuringiensis Strains and Cmentioning

confidence: 99%

See 1 more Smart Citation

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Álvarez¹,

Loto²

2012

Biodiversity Enrichment in a Diverse World

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Section: Molecular Characterization Of B Thuringiensis Strains and Csupporting

confidence: 83%

Section: Molecular Characterization Of B Thuringiensis Strains and Cmentioning

confidence: 99%

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Álvarez¹,

Loto²

2012

Biodiversity Enrichment in a Diverse World

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Section: Andendotoxin Insecticide Gene Plasmidmentioning

confidence: 97%

“…Direct evidence for the existence of plasmid-borne toxin genes in B. thuringiensis strains HD-1, HD-73, HD-2 and HD-8, was obtained by analysis of a large number of acrystalliferous mutants [7,8]. These findings were subsequently confirmed with the aid of the high frequency plasmid transfer system [14,15] israelensis Strains cured of one or more plasmids were isolated by growing the parent strain on nutrient agar at 42°C for 24 h. Plates were subsequently transferred to 30°C until sporulation was complete.…”

Section: Andendotoxin Insecticide Gene Plasmidmentioning

confidence: 99%

Assignment of the δ‐endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis

Ward,

Ellar

1983

FEBS Letters

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Plasmid analysis of Bacillus thuringiensis var. israelensis revealed the presence of at least 9 plasmids. A high frequency of plasmid loss occurred when this organism was grown at elevated temperature (42T). Analysis of over 100 isolates cured of one or more plasmids by this method revealed that loss of a 72 MDa plasmid was invariably accompanied by loss of the ability to synthesize the insecticidal kndotoxin protein. Deletion of any of the other plasmids had no effect on kndotoxin production. These results indicate the presence of a plasmid-coded copy of the structural gene for the insecticidal &endotoxin in B. thuringiensis var. israelensis.Bacillus thuringiensis var. israelensis Plasmid curing INTRODUCTIONStrains within the Gram positive bacterial species Bacillus thuringiensis are characterized by the production of protein &endotoxins that are toxic to a wide variety of Lepidopter an and some Dipteran larvae [ 11. The B-endotoxins are synthesized during sporulation as parasporal protein inclusions [2,3]. Most of the 20 serotypes of B. thuringiensis are toxic to Lepidoptera and synthesize a bipyramidal crystalline endotoxin that is refractile when viewed by phase contrast microscopy [4]. The B. thuringiensis var. israelensis endotoxin is much more irregular in shape however [5] and is extremely toxic to the larvae of certain Diptera, notably mosquitoes and blackfly [6]. Because of their potential as biological insecticides the study of these Sendotoxins is of considerable scientific and commercial interest.Recent studies [7-141 have demonstrated the presence of a complex array of plasmids in most of the insecticidal B. thuringiensis strains. Mutant strains cured of one or more plasmids are frequently no longer able to synthesize the S-endotoxin. &Endotoxin Insecticide Gene PlasmidDirect evidence for the existence of plasmid-borne toxin genes in B. thuringiensis strains HD-1, HD-73, HD-2 and HD-8, was obtained by analysis of a large number of acrystalliferous mutants [7,8]. These findings were subsequently confirmed with the aid of the high frequency plasmid transfer system [14,15]

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“…In this waYt several plasmids have been associated with crystal-protein production ( Table 6). The use of this technique is extremely important because, for most serotypes, the delta-endotoxin..bearing plasmid has a very high molecular weight and is not seen when other methods are used (Gonzalez and Carlton, 1980). k-l (HD~l); 29 x lOt).…”

Section: Nature Ofthe Delta-endotoxin Genementioning

confidence: 99%

Biochemical Genetics of the Bacterial Insect-Control AgentBacillus thuringiensis:Basic Principles and Prospects for Genetic Engineering

Dean

1984

Biotechnology and Genetic Engineering Reviews

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Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis

Cited by 121 publications

References 13 publications

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Assignment of the δ‐endotoxin gene of Bacillus thuringiensis var. israelensis to a specific plasmid by curing analysis

Biochemical Genetics of the Bacterial Insect-Control AgentBacillus thuringiensis:Basic Principles and Prospects for Genetic Engineering

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