1999
DOI: 10.1099/00207713-49-2-601
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Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli

Abstract: Sequence heterogeneities of variable positions located a t regions V1 and V6 of 56 cloned 16s rRNA genes were determined from six Escherichia coli strains. These nucleotides were involved in secondary structure base-pairing of stem-loops. Compensatory and single mutations have occurred but secondary structure was conserved. Eight different sequences were found in the stem a t region V1 indicating that in these sites mutation rates are higher than those of homogenization processes. Region V6 showed two differen… Show more

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Cited by 67 publications
(47 citation statements)
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“…Furthermore, intragenomic heterogeneity in the form of nucleotide differences between 16S rDNA copies, so-called microheterogeneity, has been described in few cases. For example, microheterogeneity has been identified in Escherichia coli (7,31), Mycobacterium terrae (34), Paenibacillus polymyxa (36), members of the class Mollicutes (14,23,39), and the Actinomycetales Thermomonospora chromogena (53), Thermobispora bispora (38), and Streptomyces spp. (49).…”
mentioning
confidence: 99%
“…Furthermore, intragenomic heterogeneity in the form of nucleotide differences between 16S rDNA copies, so-called microheterogeneity, has been described in few cases. For example, microheterogeneity has been identified in Escherichia coli (7,31), Mycobacterium terrae (34), Paenibacillus polymyxa (36), members of the class Mollicutes (14,23,39), and the Actinomycetales Thermomonospora chromogena (53), Thermobispora bispora (38), and Streptomyces spp. (49).…”
mentioning
confidence: 99%
“…Oligonucleotide primers used for PCR amplification and sequencing of the 16S rRNA gene were those described by Martínez-Murcia et al (1999). DNA was subjected to PCR amplification in a total volume of 50 ml that contained 50 mM KCl, 15 mM Tris/ HCl (pH 8.0), 1.5 mM MgCl 2 , 0.2 mM each dNTP (Amersham Biosciences), 1.25 U AmpliTaq Gold DNA polymerase (Applera) and 25 pmol each primer.…”
mentioning
confidence: 99%
“…Oligonucleotide primers used for PCR amplification and sequencing of the 16S rRNA gene were those described by Martínez-Murcia et al (1999). DNA was subjected to PCR amplification in a total volume of 50 ml that contained 50 mM KCl, 15 mM Tris/HCl (pH 8?0), 1?5 mM MgCl 2 , 0?2 mM each deoxyribonucleotide (dATP, dCTP, dGTP, dTTP; Amersham Biosciences), 1?25 U AmpliTaq Gold DNA polymerase (Applera) and 25 pmol each primer.…”
mentioning
confidence: 99%