Patterns of substrate utilization during long-term incubations at different temperatures

Steinweg, J. Megan; Plante, Alain F.; Conant, Richard T.; Paul, Eldor A.; Tanaka, D. L.

doi:10.1016/j.soilbio.2008.07.002

Cited by 182 publications

(152 citation statements)

References 24 publications

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“…Concerning the effect of temperature on CUE there was a significant apparent decrease in CUE after adding glucose when going from 9 to 19°C and this agrees with the general theory and with other studies (Farmer and Jones 1976;Frey et al 2013;Hall and Cotner 2007;Steinweg et al 2008). However, for the carbon polymer The samples collected for analysis of the consumption and synthesis of 13 C labelled compounds, which was determined based on the realtime data on total CO 2 production generated by the Respicond system (Fig.…”

Section: Discussionsupporting

confidence: 86%

“…1) ( 13 C-cellulose) there was no significant effect of temperature on CUE. Such contradictory results have also been described by Steinweg et al (2008) and Frey et al (2013), who observed a decreased CUE for the decomposition with increased temperature, while Frey et al (2013) also found that the CUE values for soil samples amended with glucose or oxalic acid did not change with the temperature. In addition, Drotz et al (2010a) reported that the CUE of glucose exhibited no temperature dependence at temperatures of +9, +4 and -4°C.…”

Section: Discussionmentioning

confidence: 52%

“…Previous studies have demonstrated that the CUE depends on a range of factors, including the temperature and nutrient availability, as well as the composition of the available carbon sources (Frey et al 2013;Manzoni et al 2012;Steinweg et al 2008). The reason why CUE is sensitive to SOM composition is that the degradation of more complex SOM structures requires a greater number of enzymatic steps (Bosatta and Ågren 1999) and hence higher investment in enzymes and also higher activation energy (Bosatta and Ågren 1999), as compared to more readily available compounds.…”

Section: Introductionmentioning

confidence: 99%

“…The increased rate of maintenance respiration at higher temperatures is attributed to increases in the amount of energy required to maintain ion gradients across the cell membrane and to the increased rate of protein turnover (Farmer and Jones 1976;Hall and Cotner 2007;Mainzer and Hempfling 1976). Despite the general understanding of how CUE is affected by temperature, studies have demonstrated contradictive results, some studies showing a limited response to an increased temperature (Devevre and Horwath 2000;Dijkstra et al 2011b) while others have shown a decreased CUE with increased temperature (Devevre and Horwath 2000;Frey et al 2013;Steinweg et al 2008). The comparatively large amount of energy required to decompose molecularly complex constituents of OM may also have a greater overall impact on the CUE than the temperature (Dijkstra et al 2011b;Wetterstedt and Ågren 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, prevailing nutrient regime and the stoichiometric balance between carbon and nutrient availability should be an important factor determining CUE of the soil microbial population. It is conceivable that some of the inconsistency in the CUE data available in the literature (Devevre and Horwath 2000;Drotz et al b;Frey et al 2013;Steinweg et al 2008) is due to differences in metabolic conditions at the time of estimating CUE (cf. Martens 1985).…”

Section: Introductionmentioning

confidence: 99%

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The effect of temperature and substrate quality on the carbon use efficiency of saprotrophic decomposition

et al. 2016

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Background and aims Mineralization of soil organic matter (SOM) constitutes a major carbon flux to the atmosphere. The carbon use efficiency (CUE) of the saprotrophic microorganisms mineralizing SOM is integral for soil carbon dynamics. Here we investigate how the CUE is affected by temperature, metabolic conditions, and the molecular complexity of the substrate. Methods We incubated O-horizon soil samples (with either 13 C-glucose or 13 C-cellulose) from a boreal coniferous forest at 4, 9, 14, and 19°C, and calculated CUEs based on the amount of 13 C-CO 2 and 13 C-labelled microbial biomass produced. The effects of substrate, temperature, and metabolic conditions (representing unlimited substrate supply and substrate limitation) on CUE were evaluated. Results CUE from metabolizing glucose was higher as compared to cellulose. A slight decrease in CUE with increasing temperature was observed in glucose amended samples (but only in the range 9-19°C), but not in cellulose amended samples. CUE differed significantly with metabolic conditions, i.e. CUE was higher during unlimited growth conditions as compared to conditions with substrate limitation. Conclusions We conclude that it is integral to account for both differences in CUE during different metabolic phases, as well as complexity of substrate, when interpreting temperature dependence on CUE in incubation studies.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of temperature and substrate quality on the carbon use efficiency of saprotrophic decomposition

et al. 2016

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Temperature sensitivity of soil microbial communities: An application of macromolecular rate theory to microbial respiration

et al. 2016

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There is compelling evidence that microbial communities vary widely in their temperature sensitivity and may adapt to warming through time. To date, this sensitivity has been largely characterized using a range of models relying on versions of the Arrhenius equation, which predicts an exponential increase in reaction rate with temperature. However, there is growing evidence from laboratory and field studies that observe nonmonotonic responses of reaction rates to variation in temperature, indicating that Arrhenius is not an appropriate model for quantitatively characterizing temperature sensitivity. Recently, Hobbs et al. (2013) developed macromolecular rate theory (MMRT), which incorporates thermodynamic temperature optima as arising from heat capacity differences between isoenzymes. We applied MMRT to measurements of respiration from soils incubated at different temperatures. These soils were collected from three grassland sites across the U.S. Great Plains and reciprocally transplanted, allowing us to isolate the effects of microbial community type from edaphic factors. We found that microbial community type explained roughly 30% of the variation in the CO2 production rate from the labile C pool but that temperature and soil type were most important in explaining variation in labile and recalcitrant C pool size. For six out of the nine soil × inoculum combinations, MMRT was superior to Arrhenius. The MMRT analysis revealed that microbial communities have distinct heat capacity values and temperature sensitivities sometimes independent of soil type. These results challenge the current paradigm for modeling temperature sensitivity of soil C pools and understanding of microbial enzyme dynamics.

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Climate Change Impacts on Net Ecosystem Productivity in a Subtropical Shrubland of Northwestern México

et al. 2018

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The sensitivity of semiarid ecosystems to climate change is not well understood due to competing effects of soil and plant‐mediated carbon fluxes. Limited observations of net ecosystem productivity (NEP) under rising air temperature and CO2 and altered precipitation regimes also hinder climate change assessments. A promising avenue for addressing this challenge is through the application of numerical models. In this work, we combine a mechanistic ecohydrological model and a soil carbon model to simulate soil and plant processes in a subtropical shrubland of northwest México. Due to the influence of the North American monsoon, the site exhibits net carbon losses early in the summer and net carbon gains during the photosynthetically active season. After building confidence in the simulations through comparisons with eddy covariance flux data, we conduct a series of climate change experiments for near‐future (2030–2045) scenarios that test the impact of meteorological changes and CO2 fertilization relative to historical conditions (1990–2005). Results indicate that reductions in NEP arising from warmer conditions are effectively offset by gains in NEP due to the impact of higher CO2 on water use efficiency. For cases with higher summer rainfall and CO2 fertilization, climate change impacts lead to an increase of ~25% in NEP relative to historical conditions (mean of 66 g C m−2). Net primary production and soil respiration derived from decomposition are shown to be important processes that interact to control NEP and, given the role of semiarid ecosystems in the global carbon budget, deserve attention in future simulation efforts of ecosystem fluxes.

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Patterns of substrate utilization during long-term incubations at different temperatures

Cited by 182 publications

References 24 publications

The effect of temperature and substrate quality on the carbon use efficiency of saprotrophic decomposition

The effect of temperature and substrate quality on the carbon use efficiency of saprotrophic decomposition

Temperature sensitivity of soil microbial communities: An application of macromolecular rate theory to microbial respiration

Climate Change Impacts on Net Ecosystem Productivity in a Subtropical Shrubland of Northwestern México

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