Payload release by liposome burst: Thermal collapse of microgels induces satellite destruction

Yaroslavov, Alexander A.; Панова, И. Г.; Sybachin, Andrey V.; Спиридонов, В. В.; Зезин, А. Б.; Mergel, Olga; Gelissen, Arjan P. H.; Tiwari, Rahul; Plamper, Felix A.; Richtering, Walter; Menger, Fredric M.

doi:10.1016/j.nano.2017.02.001

Cited by 32 publications

(10 citation statements)

References 13 publications

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“…The obtained liposomes were passed through a Sepharose CL‐4B column equilibrated with a Tris buffered saline (pH 7) to replace the extra‐liposomal solution. Subsequently, a Dox solution in a Tris buffer was added to the liposomal dispersion to achieve pH gradient‐driven Dox encapsulation that was monitored using fluorescence spectroscopy . The efficiency of Dox loading was 99%.…”

Section: Methodsmentioning

confidence: 99%

Amino‐terminated polylactide micelles with an external poly(ethylene oxide) corona as carriers of drug‐loaded anionic liposomes

Sybachin

Khlynina

Спиридонов

et al. 2018

Polymer International

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Micelles were prepared from a mixture of NH 2 -terminated poly(L-lactide) and poly(D,L-lactide)-block-poly(ethylene oxide) (molar ratio of 3:7). The micelles were complexed with bilayer lipid vesicles (liposomes) composed of anionic palmitoyloleoylphosphatidylserine and zwitterionic dioleoylphosphatidylcholine in a molar ratio of 3:7. The micelles and micelle-liposome complexes were characterized using dynamic light scattering, laser electrophoresis, fluorimetry, transmission electron microscopy, enzymatic hydrolysis and cell viability with the following main findings. (i) Average diameter of micelle cores was found to be 70 ± 10 nm. (ii) Each micelle carried ca 20 000 amino groups. (iii) In a pH 7 solution the impact of the protonated NH 2 groups in the total surface of micelles was negligible owing to their screening by bulky poly(ethylene oxide) blocks. (iv) The micelles were stable in slightly acidic and neutral aqueous solutions, but aggregated in slightly alkaline solutions. (v) The micelles showed no cytotoxicity up to 0.04 mg mL −1 concentration (the maximum concentration in the experiment). (vi) Each micelle adsorbed ca 30 anionic liposomes loaded with the antitumor antibiotic doxorubicin; the liposomes retained their integrity upon binding with micelles. (vii) The initial micelles and the micelle-liposome complexes showed two-week stability to enzymatic hydrolysis.

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Section: Methodsmentioning

confidence: 99%

Amino‐terminated polylactide micelles with an external poly(ethylene oxide) corona as carriers of drug‐loaded anionic liposomes

Sybachin

Khlynina

Спиридонов

et al. 2018

Polymer International

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“…Stimulus‐sensitive liposomes enable burst release and multiple release responding to the input of physical stimuli ( Figure A). Burst release is an attractive profile to treat cancer cells with anti‐cancer drugs . A higher dose of anti‐cancer drugs is effective to kill the cancer cells, but dosages have been constrained by the side effects of the drugs in conventional systemic delivery.…”

Section: Programmed Payload Release From Nanocapsulesmentioning

confidence: 99%

Nanocapsules for Programmed Neurotransmitter Release: Toward Artificial Extracellular Synaptic Vesicles

Sou

Sato

2019

Small

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Nanocapsules present a promising platform for delivering chemicals and biomolecules to a site of action in a living organism. Because the biological action of the encapsulated molecules is blocked until they are released from the nanocapsules, the encapsulation structure enables triggering of the topical and timely action of the molecules at the target site. A similar mechanism seems promising for the spatiotemporal control of signal transduction triggered by the release of signal molecules in neuronal, metabolic, and immune systems. From this perspective, nanocapsules can be regarded as practical tools to apply signal molecules such as neurotransmitters to intervene in signal transduction. However, spatiotemporal control of the payload release from nanocapsules persists as a key technical issue. Stimulus‐responsive nanocapsules that release payloads in response to external input of physical stimuli are promising platforms to enable programmed payload release. These programmable nanocapsules encapsulating neurotransmitters are expected to lead to new insights and perspectives related to artificial extracellular synaptic vesicles that might provide an experimental and therapeutic strategy for neuromodulation and nervous system disorders.

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“…The other effective factor is the shrinkage of the PAA segments in response to pH reduction. This particle shrinkage exerts a force which further drives the drug molecules outside the nanoparticle (40). In this sense, the degree of cross-linking of the nanoparticles plays a role; as the cross-linker content increases, the drug molecules are faced a greater prevention against diffusion outside the particle due to more rigid structure of the nanoparticle.…”

Section: Drug Loading and Drug Release Studiesmentioning

confidence: 99%

Stimuli-responsive DOX release behavior of cross-linked poly(acrylic acid) nanoparticles

2019

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Cross-linked poly(acrylic acid) nanoparticles were synthesized via distillation precipitation polymerization of acrylic acid and ethylene glycol dimethacrylate withdifferent molar ratios. Spherical nanoparticles with diameters between 75 and 122 nm were synthesized and exhibited temperature and pH-responsive behaviors. However, this behavior was less pronounced for samples with higher cross-linking degrees. The potential of all nanoparticles as carriers for controlled release of doxorubicin (DOX) anti-cancer drug was examined at pH values of 1.2, 5.3 and 7.4. An obvious alleviation in burst release behavior and the amount of cumulative drug release was seen for all nanoparticles as the pH of the medium and the cross-linking degree of nanoparticle increased. Also kinetics of drug release was studied using mathematical models of zero-order, first-order, Higuchi, Korsmeyer-Peppas and Hixson-Crowell, where Higuchi and Korsmeyer-Peppas models best defined the kinetics of drug release.

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Payload release by liposome burst: Thermal collapse of microgels induces satellite destruction

Cited by 32 publications

References 13 publications

Amino‐terminated polylactide micelles with an external poly(ethylene oxide) corona as carriers of drug‐loaded anionic liposomes

Amino‐terminated polylactide micelles with an external poly(ethylene oxide) corona as carriers of drug‐loaded anionic liposomes

Nanocapsules for Programmed Neurotransmitter Release: Toward Artificial Extracellular Synaptic Vesicles

Stimuli-responsive DOX release behavior of cross-linked poly(acrylic acid) nanoparticles

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