PBP genes regulated by the development of the ovaries, sex pheromone release, mating and oviposition behavior in Conogethes punctiferalis (Guenée)

Jing, Dapeng; Zhang, Tiantao; Prabu, Sivaprasath; Bai, Shuxiong; He, Kanglai; Luan, Jing; Wang, Zhenying

doi:10.1007/s00049-020-00323-3

Cited by 3 publications

(5 citation statements)

References 44 publications

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“…In fact, many moths have shown plasticity of olfactory-guided behavior, dependent not only on the nature of the chemical but also on the physiological status (e.g., age, growth conditions, hormone or mating status) of the individual (Anton et al, 2007;Kubli, 2003;Jing et al, 2020). Our previous studies have also proved this result by stimulating host odorants, ovarian development, and changes in GOBPs expression before and after oviposition (Jing et al, 2019;Jing et al, 2020). For males and looking for hosts to maintain normal physiological activities after emergence, it is more important to find females to complete mating.…”

Section: Discussionmentioning

confidence: 99%

Genetic knockout and general odorant binding/chemosensory proteins interactions: revealed the function and importance of GOBP2 in the yellow peach moth olfactory system

Jing,

Prabu,

Zhang

et al. 2024

Preprint

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The GOBP2 protein has a unique function in the yellow peach moth, Conogethes punctiferalis. Several general odorant binding proteins (GOBPs) have been identified in various lepidopteran species, but the functional difference between GOBP1 and GOBP2 in recognition of host plant odorants is still unknown. The functions of GOBP1 and GOBP2 in the yellow peach moth were evaluated in this study using the CRISPR-Cas9 system. The results revealed the importance of GOBP2 in the olfaction mechanism in the yellow peach moth. The GOBP1 knocked out larvae’s perception towards feeding decreased but did not reach a significant level, while knocking out the GOBP2 and GOBP1/2 genes resulted in huge differences. On the other hand, electroantennograms (EAG) and wind tunnel tests showed that the GOBP2 knocked out adult’s sensitivity to odorants decreased more than GOBP1 knocked out individuals. The STRING database text mining results grabbed our attention in the protein-protein interaction studies. In this research, we firstly proved the existence of physical interactions between GOBPs and chemosensory proteins (CSP) through the surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) methods. Interestingly, the GOBP1 and GOBP2 could not interact with each other, but they can interact with CSPs. The interaction result indicates that GOBP2 could physically interact with CSP15, CSP5, and OBP17, whereas GOBP1 could bind only with CSP5 and CSP10, and the association constant (ka) is also more substantial than GOBP1. These results strongly suggest the importance of GOBP2 function in the perception of host plants odorants in the yellow peach moth.

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Section: Discussionmentioning

confidence: 99%

Genetic knockout and general odorant binding/chemosensory proteins interactions: revealed the function and importance of GOBP2 in the yellow peach moth olfactory system

Jing,

Prabu,

Zhang

et al. 2024

Preprint

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“…In summary, supplemental nutrition was important for oviposition of C. punctiferalis after emergence, and 10% sucrose water was the most effective for growth and reproduction. Jing et al (2021) studied the ovarian development of C. punctiferalis in different periods. Building on Jing et al (2021), we explored the effects of nutrient availability on ovarian development regarding these periods.…”

Section: Discussionmentioning

confidence: 99%

“…Jing et al (2021) studied the ovarian development of C. punctiferalis in different periods. Building on Jing et al (2021), we explored the effects of nutrient availability on ovarian development regarding these periods. The results showed that ovaries of C. punctiferalis develop even if the newly emerged female moths were not supplemented with nutrients.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…According to the method of Zhang et al (2021), ovarian development in C. punctiferalis can be divided into 5 developmental stages: milky transparent stage, yolk deposition stage, mature waiting period, peak oviposition period, and late oviposition period. Milky white eggs referred to the description of egg development in Jing et al (2021). The eggs at lateral and middle oviducts were considered to be mature eggs.…”

Section: Effects Of Nutrients On Ovarian Development In C Punctiferalismentioning

confidence: 99%

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Effects of supplemental nutrients on ovarian development and oviposition in Conogethes punctiferalis (Lepidoptera: Crambidae)

Zhang

Jiang

et al. 2023

Environmental Entomology

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Conogethes punctiferalis is a serious pest in China affecting a wide variety of field crops, fruits, and forest trees. Many insects require supplemental nutrients after emergence to compensate for insufficient nutrients at the larval stage. In this study, to better understand the determinants of C. punctiferalis survival and reproduction, the impact of supplemental nutrition was examined. In particular, the effects of 11 treatments (5% sucrose, 10% sucrose, 15% sucrose, 5% sophora honey, 10% sophora honey, 15% sophora honey, 5% wild honey, 10% wild honey, 15% wild honey, distilled water, and blank control) on adult longevity and oviposition were evaluated, with detailed morphological analyses of ovarian development in the 10% sucrose, distilled water, and blank control groups. Conogethes punctiferalis required supplementary nutrition after emergence. Supplementary nutrition improved fecundity (number of eggs laid) and longevity, and 10% sucrose water had the greatest effects. The preoviposition period and oviposition period of C. punctiferalis females were longer, and the numbers of eggs in female ovarian tubes and eggs laid by females were higher in the 10% sucrose water group than in other groups. Females supplemented with distilled water laid a small number of eggs. Without nutrient supplementation, females did not lay eggs. In conclusion, supplemental nutrition was beneficial for ovarian development in female moths, prolonging the oviposition period and lifespan, and was an important factor affecting population dynamics. These results lay a foundation for further analyses of the nutritional requirements for C. punctiferalis in the field and provide a reference for indoor population feeding.

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Genetic knockout and general odorant-binding/chemosensory protein interactions: Revealing the function and importance of GOBP2 in the yellow peach moth's olfactory system

Jing

Prabu

Zhang

et al. 2021

International Journal of Biological Macromolecules

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PBP genes regulated by the development of the ovaries, sex pheromone release, mating and oviposition behavior in Conogethes punctiferalis (Guenée)

Cited by 3 publications

References 44 publications

Genetic knockout and general odorant binding/chemosensory proteins interactions: revealed the function and importance of GOBP2 in the yellow peach moth olfactory system

Genetic knockout and general odorant binding/chemosensory proteins interactions: revealed the function and importance of GOBP2 in the yellow peach moth olfactory system

Effects of supplemental nutrients on ovarian development and oviposition in Conogethes punctiferalis (Lepidoptera: Crambidae)

Genetic knockout and general odorant-binding/chemosensory protein interactions: Revealing the function and importance of GOBP2 in the yellow peach moth's olfactory system

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