PCB 77 action in ovary cells – toxic effects, apoptosis induction and cell cycle analysis

Abstract

These results indicate toxic outcomes of PCB 77 at ovarian cellular level with regard to potential direct adverse effects to female reproductive system.

Ortho-substituted PCB 153: effects in CHO-K1 cells

“…Time dependency was also evident in the increase in the apoptotic peak at 48 h (Figure 4A). We reported similar findings for PCB 77 earlier (13) and Venkatesha et al ( 5) also reported that PCB 153 inhibited the entry of MCF-10A human mammary epithelial cells into the S-phase, while the proportion of G1 cells remained high (90-95 %).…”

“…Variations in IC 50 values between the four assays suggest that combining multiple assays with different endpoints (such as membrane integrity, lysosomal activity, total cellular protein content, and mitochondrial function) can provide valuable information about possible mechanisms of action of the tested compound. Compared to the IC 50 values obtained for planar dioxin-like PCB 77 from our previous study (13), non-planar di-orthosubstituted PCB 153 in this study (Table 1) showed a much weaker toxic effect on ovarian cells, especially after prolonged exposure.…”

“…PCBs are known to disrupt the endocrine, immune, nervous, and reproductive systems ( 13 , 14 ), but because most such studies have been carried out with Aroclor mixtures, little is known about the exact mechanisms of action of individual PCBs, particularly with respect to the ovaries. Sechman et al ( 15 ) have reported that PCB 153 disrupts follicular steroidogenesis, oestrogen receptor expression, and recruitment of small yellow follicles into the preovulatory hierarchy.…”

“…In our previous study we reported the molecular mechanisms of one of the most toxic PCB congeners – dioxin-like PCB 77 in Chinese hamster ovary K1 cells ( 13 ). The aim of this study was to expand research to PCB 153, as it differs from PCB 77 in the degree of chlorination, planarity, and activity.…”

Cell membrane‐related toxic responses and disruption of intercellular communication in PCB mechanisms of toxicity: A review

“…Results have shown that PCB 153 triggers a homeoviscous response by changing cholesterol content in liver (+51%) and brain (+216%), as well as the double bond index in gills (−17%). Previous study conducted in our laboratory (Murati et al, 2015) suggests specific damage in the cellular membranes, and alterations affecting the proliferation, metabolism and/or adhesion of ovarian cells after PCB 77 treatments. Our group also investigated correlation of PCB cytotoxicity with chlorine substitution and pointed out statistically significant cytotoxicity after exposure to both (DL and ortho ‐substituted) PCBs with slightly profound effects of DL PCB 77.…”