PCL/chitosan/Zn-doped nHA electrospun nanocomposite scaffold promotes adipose derived stem cells adhesion and proliferation

Ghorbani, F.; Kaffashi, Babak; Shokrollahi, Parvin; Seyedjafari, Ehsan; Ardeshirylajimi, Abdolreza

doi:10.1016/j.carbpol.2014.10.071

Cited by 180 publications

(87 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This supports the findings of Wang et al (2015) who reported greater osteoblast adhesion to chitosan-collagen blend films [56]. Moreover, similar effects were obtained with adipose stem cells cultured onto poly(ε-caprolactone)-chitosan material [57]. The enhanced adhesion we showed may lead to cell growth.…”

Section: Resultssupporting

confidence: 91%

Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion

et al. 2015

View full text Add to dashboard Cite

Designing scaffolds made from natural polymers may be highly attractive for tissue engineering strategies. We sought to produce and characterize chitosan-coated collagen membranes and to assess their efficacy in promoting chondrocyte adhesion, growth, and cytokine secretion. Porous collagen membranes were placed in chitosan solutions then crosslinked with glutaraldehyde vapor. Fourier transform infrared (FTIR) analyses showed elevated absorption at 1655 cm−1 of the carbon–nitrogen (N=C) bonds formed by the reaction between the (NH2) of the chitosan and the (C=O) of the glutaraldehyde. A significant peak in the amide II region revealed a significant deacetylation of the chitosan. Scanning electron microscopy (SEM) images of the chitosan-coated membranes exhibited surface variations, with pore size ranging from 20 to 50 μm. X-ray photoelectron spectroscopy (XPS) revealed a decreased C–C groups and an increased C–N/C–O groups due to the reaction between the carbon from the collagen and the NH2 from the chitosan. Increased rigidity of these membranes was also observed when comparing the chitosan-coated and uncoated membranes at dried conditions. However, under wet conditions, the chitosan coated collagen membranes showed lower rigidity as compared to dried conditions. Of great interest, the glutaraldehyde-crosslinked chitosan-coated collagen membranes promoted chondrocyte adhesion, growth, and interleukin (IL)-6 secretion. Overall results confirm the feasibility of using designed chitosan-coated collagen membranes in future applications, such as cartilage repair.

show abstract

Section: Resultssupporting

confidence: 91%

Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Many attempts have been made to construct dynamic hydrogels based on Schiff bases using natural biopolymers, among which chitosan and its derivatives occupied the largest share because of desirable properties such as biocompatibility, biodegradability, and antimicrobial activity . Zhang et al .…”

Section: Introductionmentioning

confidence: 99%

Self‐Healing, Injectable Gelatin Hydrogels Cross‐Linked by Dynamic Schiff Base Linkages Support Cell Adhesion and Sustained Release of Antibacterial Drugs

Vahedi

Barzin

Shokrolahi

et al. 2018

Macro Materials & Eng

Self Cite

View full text Add to dashboard Cite

A new class of dynamic hydrogels made through Schiff base bonds based on gelatin (type A and B) and polyethylene glycol dibenzaldehyde (diBA‐PEG, 2000 and 4000 g mol−1) is developed. Hydrogels form in situ by mixing aqueous solutions of gelatin and diBA‐PEG at a carefully adjusted pH. Compression test shows that the samples based on gelatin A are able to withstand at least ten cyclic loading/unloading without crack formation and significant permanent deformation. Self‐healing behavior of the hydrogel is proved by rheological measurements and also visual method. This hydrogel is proven to be injectable and nontoxic. Performance of the hydrogel in loading and delivery of clindamycin hydrochloride, as an antibacterial model drug, is evaluated against Staphylococcus aureus via antibacterial activity test. In vitro release of clindamycin hydrochloride is studied through an innovative method and it becomes clear that the release of clindamycin hydrochloride from this hydrogel follows a zero‐order kinetics.

show abstract

“…The peak seen at 698 cm −1 also represents PO 4 3− vibration. The strong peak at 1110 cm −1 corresponds to PO 4 3− group which has been sharpened after soaking in SBF …”

Section: Resultsmentioning

confidence: 99%

“…The strong peak at 1110 cm −1 corresponds to PO 4 3− group which has been sharpened after soaking in SBF. 31 Consequently, phosphate group exists on the surface of the scaffold that shows hydroxyapatite formation. Other hydroxyapatite related peaks have been overlapped with the characteristic peaks of the main component (PLLA) and are not visible in the FTIR spectra, but the existence of hydroxyapatite has been confirmed using further assays.…”

Section: Scaffold Characterizationmentioning

confidence: 99%

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Ataie

Shabani

Seyedjafari

2018

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Surface mineralized nanofibrous scaffolds hold great potential for bone tissue engineering applications. In this study, a new hybrid nanofibrous scaffold composed of alginate/poly(l‐lactide) nanofibers was fabricated using electrospinning method and then crosslinking process was employed. Hydroxyapatite crystal formation took place using in situ precipitation by immersion of the scaffolds in simulated body fluid solution for 10 days at 37°C. The morphologies of the scaffolds were observed using scanning electron microscope. Hydroxyapatite crystal formation on the surface of nanofibers was confirmed using scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy and X‐ray diffraction analysis. The biocompatibility of the fabricated scaffolds was evaluated using mesenchymal stem cell culture and MTT assay. According to alkaline phosphatase activity and calcium content assays, it was concluded that the osteogenic differentiation of stem cells was considerable on scaffolds containing hydroxyapatite crystals in comparison with other specimens. The results showed biocompatibility of the scaffolds and their support from stem cell adhesion, growth and osteogenic differentiation, so the hydroxyapatite‐coated poly(l‐lactide)/alginate hybrid nanofibrous scaffolds hold suitable characteristics for bone tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 586–596, 2019.

show abstract

PCL/chitosan/Zn-doped nHA electrospun nanocomposite scaffold promotes adipose derived stem cells adhesion and proliferation

Cited by 180 publications

References 38 publications

Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion

Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion

Self‐Healing, Injectable Gelatin Hydrogels Cross‐Linked by Dynamic Schiff Base Linkages Support Cell Adhesion and Sustained Release of Antibacterial Drugs

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Contact Info

Product

Resources

About