PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: Morphology, mechanical properties and bioactivity

Milovac, Dajana; Ferrer, Gloria Gallego; Ivanković, Marica; Ivanković, Hrvoje

doi:10.1016/j.msec.2013.09.036

Cited by 121 publications

(79 citation statements)

References 51 publications

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“…The resultant compressive stress-strain curves, shown in Figure 7, demonstrate that the scaffolds presented the typical stress versus strain response of highly porous polymer scaffolds [12, 36]. The obtained curves are characterized by three different regions: a linear region at lower strain values, suggesting an initial rigid mechanical response, associated with elastic behaviour of the scaffolds; a region with lower stiffness; and, lastly, a region where a rise of stress with increasing strain is noticed, which is related to densification of the porous scaffolds [12, 35, 37].…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Morouço

Biscaia

Viana

et al. 2016

BioMed Research International

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Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues. In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP). The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed. Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured. The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds. Significant effects on surface wettability and thermal properties were observed, among scaffolds. Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained. Results from the cytotoxicity assay suggest that all the composite scaffolds presented good biocompatibility. The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.

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Section: Resultsmentioning

confidence: 99%

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Morouço

Biscaia

Viana

et al. 2016

BioMed Research International

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“…Bone scaffolds should possess similar compressive strength to the host bone and provide support until complete regeneration and functional restoration of the damaged host bone tissue. Number of studies have incorporated synthetic materials or biopolymers with HA because HA alone is a brittle material with low fracture toughness [32, 33]. Researchers have also conducted studies using HA as an additional constituent in scaffolds to strengthen natural polymers [7, 34].…”

Section: Introductionmentioning

confidence: 99%

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

Uswatta

Okeke

Jayasuriya

2016

Materials Science and Engineering: C

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In this study we have fabricated porous injectable spherical scaffolds using chitosan biopolymer, sodium tripolyphosphate (TPP) and nano-hydroxyapatite (nHA). TPP was primarily used as an ionic crosslinker to crosslink nHA/chitosan droplets. We hypothesized that incorporating nHA into chitosan could support osteoconduction by emulating the mineralized cortical bone structure, and improve the Ultimate Compressive Strength (UCS) of the scaffolds. We prepared chitosan solutions with 0.5%, 1% and 2% (w/v) nHA concentration and used simple coacervation and lyophilization techniques to obtain spherical scaffolds. Lyophilized spherical scaffolds had a mean diameter of 1.33 mm (n=25). Further, portion from each group lyophilized scaffolds were soaked and dried to obtain Lyophilized Soaked and Dried (LSD) scaffolds. LSD scaffolds had a mean diameter of 0.93 mm (n=25) which is promising property for the injectability. Scanning Electron Microscopy images showed porous surface morphology and interconnected pore structures inside the scaffolds. Lyophilized and LSD scaffolds had surface pores less than 10 and 2 µm, respectively. 2% nHA/chitosan LSD scaffolds exhibited UCS of 8.59 MPa compared to UCS of 2% nHA/chitosan lyophilized scaffolds at 3.93 MPa. Standardize UCS values were 79.98 MPa and 357 MPa for 2% nHA/chitosan lyophilized and LSD particles respectively. One-way ANOVA results showed a significant increase (p < 0.001) in UCS of 1% and 2% nHA/chitosan lyophilized scaffolds compared to 0% and 0.5% nHA/chitosan lyophilized scaffolds. Moreover, 2% nHA LSD scaffolds had significantly increased (p < 0.005) their mean UCS by 120% compared to 2% nHA lyophilized scaffolds. In a drawback, all scaffolds have lost their mechanical properties by 95% on the 2nd day when fully immersed in phosphate buffered saline. Additionally live and dead cell assay showed no cytotoxicity and excellent osteoblast attachment to both lyophilized and LSD scaffolds at the end of 14th day of in vitro studies. 2% nHA/chitosan scaffolds showed higher osteoblast attachment than 0% nHA/chitosan scaffolds.

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“…[5][6][7] But they have poor mechanical properties like low fracture toughness, which creates space to find alternative bioceramics. 8,9 Thus, wollastonite is considered an ideal candidate for artificial bone implants in hard tissue regeneration and bone tissue engineering 10,11 and is also widely used in drug delivery applications. 12 The formation rate of hydroxyapatite on the surface of the wollastonite proved to be faster than that of any other biocompatible glasses, glass ceramics, and calcium phosphates due to the presence of silicate group which actively takes part in the metabolic processes during bone formation.…”

Section: Introductionmentioning

confidence: 99%

Influence of needle-like morphology on the bioactivity of nanocrystalline wollastonite – an in vitro study

Lakshmi¹,

Swamiappan²

2015

IJN

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In the past 2 decades, wollastonite has been studied thoroughly for its application as a bone implant material due to its biocompatibility, high mechanical strength, and excellent bioactivity when compared to calcium phosphates bioceramics. Wollastonite was prepared through the low-temperature sol-gel combustion method using urea as the fuel, nitrate ions and nitric acid as the oxidizer. Calcium nitrate and tetraethyl orthosilicate were taken as the source of calcium and silica. The synthesized wollastonite were characterized by Fourier transform infrared spectroscopy for the identification of characteristic functional group and powder X-ray diffraction for the phase identification. Employing urea as a fuel resulted in needle-like morphology of the particles, which was confirmed by scanning electron microscopy and transmission electron microscopy. It was observed that the needle-like morphology enhances the mechanical properties such as elasticity and compressive strength and also increases the surface area of the material, which could help in a rapid deposition of hydroxyapatite layer. These properties of wollastonite warrant its application as a new artificial bone material in the field of hard tissue engineering.

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PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: Morphology, mechanical properties and bioactivity

Cited by 121 publications

References 51 publications

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

Influence of needle-like morphology on the bioactivity of nanocrystalline wollastonite – an in vitro study

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PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: Morphology, mechanical properties and bioactivity

Cited by 121 publications

References 51 publications

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

Influence of needle-like morphology on the bioactivity of nanocrystalline wollastonite &ndash; an in vitro study

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Influence of needle-like morphology on the bioactivity of nanocrystalline wollastonite – an in vitro study