PCL NGCs integrated with urolithin-A-loaded hydrogels for nerve regeneration

Jin, Xue-Han; Fang, Jia-Qi; Wang, Jianguang; Xu, Bo; Wang, Xu; Liu, Shuhao; Chen, Feng; Liu, Junjian

doi:10.1039/d2tb01624a

Cited by 8 publications

(4 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a close connection between oxidative stress and peripheral nerve regeneration. [46][47][48] Macrophages release antiinflammatory factors, which can promote RSC proliferation, axonal regeneration, and myelination during recovery. [42] Hypertrophic scars can arise from permanent inflammation, [49] such that control of oxidative attacks and inflammatory responses takes on great significance in nerve injury recovery.…”

Section: Discussionmentioning

confidence: 99%

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Fang,

Xu,

Jin

et al. 2024

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

Peripheral nerve injuries represent a prevalent and severe category of damage resulting from traumatic incidents. Predominantly, the deficiency in nerve regeneration can be ascribed to enduring inflammatory reactions, hence imposing substantial clinical implications for patients. Fisetin, a flavonoid derived from plants, is naturally present in an array of vegetables and fruits, including strawberries, apples, onions, and cucumbers. It exhibits immunomodulatory properties through the reduction of inflammation and oxidative stress. In the present research, a nerve defect was addressed for the first time utilizing a scaffold primed for controlled fisetin release. In this regard, fisetin‐loaded chitosan hydrogels were incorporated into the lumen of polycaprolactone (PCL) nerve guide conduits (NGCs). The hydrogel maintained a steady release of an appropriate fisetin dosage. The study outcomes indicated that the fisetin/chitosan/polycaprolactone (FIS/CS/PCL) NGCs amplified Schwann cell proliferation and neural expression, curtailed oxidative stress, alleviated inflammation, and improved functions, electrophysiological properties, and morphology. This pioneering scaffold has potential to contribute significantly to the field of neuroengineering.This article is protected by copyright. All rights reserved

show abstract

Section: Discussionmentioning

confidence: 99%

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Fang,

Xu,

Jin

et al. 2024

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other factors can be added to reduce the aversiveness of the injured microenvironment and better support endogenous nerve repair. For example, a 2022 study by Jin et al observed that urolithin-A-loaded hydrogels in PCL conduits exhibited anti-inflammatory, antioxidant, and immunomodulatory effects [ 86 ].…”

Section: Biomaterials Modifications Influence Cell Behaviormentioning

confidence: 99%

Interactions of Cells and Biomaterials for Nerve Tissue Engineering: Polymers and Fabrication

Harley-Troxell,

Steiner,

Advincula

et al. 2023

Polymers

View full text Add to dashboard Cite

Neural injuries affect millions globally, significantly impacting their quality of life. The inability of these injuries to heal, limited ability to regenerate, and the lack of available treatments make regenerative medicine and tissue engineering a promising field of research for developing methods for nerve repair. This review evaluates the use of natural and synthetic polymers, and the fabrication methods applied that influence a cell’s behavior. Methods include cross-linking hydrogels, incorporation of nanoparticles, and 3D printing with and without live cells. The endogenous cells within the injured area and any exogenous cells seeded on the polymer construct play a vital role in regulating healthy neural activity. This review evaluates the body’s local and systemic reactions to the implanted materials. Although numerous variables are involved, many of these materials and methods have exhibited the potential to provide a biomaterial environment that promotes biocompatibility and the regeneration of a physical and functional nerve. Future studies may evaluate advanced methods for modifying material properties and characterizing the tissue–biomaterial interface for clinical applications.

show abstract

“…[ 1 ] The main causes of the poor prognosis of PNI treatment are interference from surrounding muscle tissue and inflammation. [ 2 ] The main strategy for PNI treatment via nerve guide conduits (NGCs) is to provide ideal mechanical support for nerve regeneration and prevent peripheral tissues from interfering with nerve repair. Although NGCs can block peripheral inflammatory cell invasion, the inflammatory microenvironment (IME) in NGCs can inhibit the functional recovery of Schwann cells and neurons.…”

Section: Introductionmentioning

confidence: 99%

Remodeling of the Intra‐Conduit Inflammatory Microenvironment to Improve Peripheral Nerve Regeneration with a Neuromechanical Matching Protein‐Based Conduit

Wang,

Yuan,

Zhang

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Peripheral nerve injury (PNI) remains a challenging area in regenerative medicine. Nerve guide conduit (NGC) transplantation is a common treatment for PNI, but the prognosis of NGC treatment is unsatisfactory due to 1) neuromechanical unmatching and 2) the intra‐conduit inflammatory microenvironment (IME) resulting from Schwann cell pyroptosis and inflammatory‐polarized macrophages. A neuromechanically matched NGC composed of regenerated silk fibroin (RSF) loaded with poly(3,4‐ethylenedioxythiophene): poly(styrene sulfonate) (P:P) and dimethyl fumarate (DMF) are designed, which exhibits a matched elastic modulus (25.1 ± 3.5 MPa) for the peripheral nerve and the highest 80% elongation at break, better than most protein‐based conduits. Moreover, the NGC can gradually regulate the intra‐conduit IME by releasing DMF and monitoring sciatic nerve movements via piezoresistive sensing. The combination of NGC and electrical stimulation modulates the IME to support PNI regeneration by synergistically inhibiting Schwann cell pyroptosis and reducing inflammatory factor release, shifting macrophage polarization from the inflammatory M1 phenotype to the tissue regenerative M2 phenotype and resulting in functional recovery of neurons. In a rat sciatic nerve crush model, NGC promoted remyelination and functional and structural regeneration. Generally, the DMF/RSF/P:P conduit provides a new potential therapeutic approach to promote nerve repair in future clinical treatments.

show abstract

PCL NGCs integrated with urolithin-A-loaded hydrogels for nerve regeneration

Abstract: Inflammation and oxidative stress are among the leading causes of poor prognosis after peripheral nerve injury (PNI). Urolithin-A (UA),¬ an intermediate product produced by catabolism of ellagitannins in the gastrointestinal...

Cited by 8 publications

References 64 publications

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Interactions of Cells and Biomaterials for Nerve Tissue Engineering: Polymers and Fabrication

Remodeling of the Intra‐Conduit Inflammatory Microenvironment to Improve Peripheral Nerve Regeneration with a Neuromechanical Matching Protein‐Based Conduit

Contact Info

Product

Resources

About