PCR amplification using a single cell allows the detection of the mtDNA lesion associated with Leber's hereditary optic neuropathy

Erickson, Catherine E.; Castora, Frank J.

doi:10.1016/0925-4439(93)90093-g

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Each drop was subsequently examined microscopically (100-200 × magnification) for the presence of a single cell, and transferred to a reaction vessel containing 9 µl of sterile water. Samples were heated for 10 min at 95°C, centrifuged for 5 min at 12 000 g max , and supernatants used for PCR amplification (Erickson and Castora 1993).…”

Section: Methodsmentioning

confidence: 99%

Preferential amplification and phenotypic selection in a population of deleted and wild-type mitochondrial DNA in cultured cells

et al. 1997

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Section: Methodsmentioning

confidence: 99%

Preferential amplification and phenotypic selection in a population of deleted and wild-type mitochondrial DNA in cultured cells

et al. 1997

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“…The cells were lysed by incubation at 95°C for 10 minutes with occasional shaking to liberate the total DNA. 16 Following single granulocyte deposition into each well of a 96-well microplate containing 30 L 1 ϫ TE buffer, total DNA was extracted from single granulocytes by incubation at 95°C for 10 minutes in the 96-well reaction plate (Applied Biosystems) with use of the GeneAmp PCR system 9700 (Applied Biosystems). The lysate was briefly centrifuged and stored at Ϫ20°C.…”

Section: Dna Extraction From Individual Cd34 Clones and Single Granulmentioning

confidence: 99%

Mitochondrial DNA sequence heterogeneity in circulating normal human CD34 cells and granulocytes

Shin

Tarnowka²,

McCoy³

et al. 2004

Blood

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We have reported marked mitochondrial DNA (mtDNA) sequence heterogeneity among individual CD34 clones from adult bone marrow (BM) and the age-dependent accumulation of mtDNA mutations in this mitotically active tissue. Here, we show direct evidence of clonal expansion of cells containing mtDNA mutations and that the mtDNA sequence may be easily determined by using peripheral blood (PB) as a CD34 cell source. Analysis of 594 circulating CD34 clones showed that 150 (25%) had mtDNA sequences different from the same donor's corresponding aggregate sequence. Examination of single granulocytes indicated that 103 (29%) from the same 6 individuals showed mtDNA heterogeneity, with sequences distinct from the corresponding aggregate tissue sequence and from the sequences of other single granulocytes. Circulating and BM CD34 cells showed virtually identical patterns of mtDNA heterogeneity, and the same changes were seen in progeny granulocytes as in their progenitors, indicating that blood sampling could be used in studies to determine whether mtDNA reflects an individual's cumulative or recent exposure to mutagens; as a marker of individual hematopoietic progenitors, stem cells, and their expansion; and for the detection of minimal residual disease in hematologic malignancies of

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An age-associated correlation between cellular bioenergy decline and mtDNA rearrangements in human skeletal muscle

Kopsidas

Kovalenko

Kelso

et al. 1998

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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PCR amplification using a single cell allows the detection of the mtDNA lesion associated with Leber's hereditary optic neuropathy

Cited by 3 publications

References 19 publications

Preferential amplification and phenotypic selection in a population of deleted and wild-type mitochondrial DNA in cultured cells

Preferential amplification and phenotypic selection in a population of deleted and wild-type mitochondrial DNA in cultured cells

Mitochondrial DNA sequence heterogeneity in circulating normal human CD34 cells and granulocytes

An age-associated correlation between cellular bioenergy decline and mtDNA rearrangements in human skeletal muscle

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