PCR-based clonality analysis of antigen receptor gene rearrangements in canine cutaneous plasmacytoma

Takanosu, Masamine; Okada, K; Kagawa, Yumiko

doi:10.1016/j.tvjl.2018.09.010

Cited by 12 publications

(15 citation statements)

References 19 publications

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“…2,3,5,7,18 This overlap is further complicated by virtually identical immunophenotypic profiles, even when an exhaustive panel of immunomarkers is used. 20 In agreement with previous studies, plasmablastic lymphomas have a post-germinal center B-cell/plasma cell phenotype, expressing MUM1/IRF4 and CD138/syndecan-1, but not CD20, 3,24,26 unlike other large B-cell lymphomas that usually express the pan-B cell antigens, CD20, CD79a, and PAX-5. 8,22 In these studies, several markers that may be aberrantly expressed in plasma cell neoplasms (i.e., CD56, CD10, and CD4) were also expressed in most cases of plasmablastic lymphoma.…”

supporting

confidence: 91%

“…1,4,21,27 PARR confirmed that the tumor was negative for both B and T clonality as previously reported in canine plasma cell tumors. 20,25 Combining morphologic and immunophenotypic observations, our final diagnosis was disseminated plasma cell neoplasm at the leukemic stage. Taking into consideration the plasmablastic morphology of the cells, plasma cell leukemia (primary or secondary to multiple myeloma) and plasmablastic lymphoma at leukemic stage were the 2 differentials.…”

mentioning

confidence: 91%

“…1,4,21,27 PARR confirmed that the tumor was negative for both B and T clonality as previously reported in canine plasma cell tumors. 20,25…”

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confidence: 99%

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Plasma cell leukemia with plasmablastic morphology in a dog

et al. 2019

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A 5-y-old female Golden Retriever was presented with a 2-wk history of hyporexia, vomiting, diarrhea, lethargy, weight loss, polyuria, and polydipsia. Clinical examination and ultrasonography revealed multiple organ enlargement with gallbladder and kidney nodules suggestive of disseminated neoplasia. Hematologic and biochemical analyses revealed pancytopenia, hypercalcemia, and monoclonal IgA gammopathy suspicious for a plasma cell neoplasm. Bone marrow and blood smear examination revealed neoplastic atypical cells highly suggestive of lymphoid origin. Autopsy confirmed the presence of homogeneous white masses and multifocal pale infiltrates in the spleen, kidney, small intestine, gallbladder, and urinary tract. Histologic features were consistent with a multicentric atypical plasma cell tumor. Tumor cells were negative for CD204, IBA-1, E-cadherin, CD3, CD5, CD79a, CD20, and PAX5, and positive for MUM1, consistent with plasma cell origin. The presence of > 20% of circulating blastic plasma cells was consistent with primary plasma cell leukemia with plasmablastic morphology, a disease rarely described in veterinary medicine.

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Plasma cell leukemia with plasmablastic morphology in a dog

et al. 2019

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“…38 PCR analysis of antigen receptor gene rearrangement (PARR) has been proposed as an adjunct tool to detect clonality in PCTs. 27,32,33 A PARR based on Ig κ locus demonstrated higher sensitivity compared to the Ig heavy chain locus, although limited literature is available about its specificity. 32,33 In human pathology, IHC for light chains is now commonly replaced or associated with RNA in situ hybridization (ISH), which has been proven more sensitive to multiple myeloma, the most common plasma cell neoplasm in humans, and non-Hodgkin B-cell lymphomas.…”

Section: Introductionmentioning

confidence: 99%

“…27,32,33 A PARR based on Ig κ locus demonstrated higher sensitivity compared to the Ig heavy chain locus, although limited literature is available about its specificity. 32,33 In human pathology, IHC for light chains is now commonly replaced or associated with RNA in situ hybridization (ISH), which has been proven more sensitive to multiple myeloma, the most common plasma cell neoplasm in humans, and non-Hodgkin B-cell lymphomas. 4,7,34 To our knowledge, no studies investigating the applicability of ISH for Ig light chains in canine PCTs have been reported.…”

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confidence: 99%

Chromogenic in situ hybridization for the detection of lambda and kappa immunoglobulin light chains as a potential auxiliary diagnostic technique in canine plasmacytomas

et al. 2020

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The heterogeneous morphologic features of canine plasmacytomas (PCTs) can make their differentiation from other round cell tumors challenging. Immunohistochemistry (IHC) for lambda (λ) and kappa (к) immunoglobulin (Ig) light chains is often equivocal because of high background staining. The chromogenic in situ hybridization (CISH) technique for light chains has shown higher sensitivity compared to IHC in human plasma cell tumors. Therefore, we aimed to validate automated CISH for light chains in canine tissues and to evaluate its diagnostic potential in canine PCTs, in conjunction with routinely used IHC markers. CISH for light chains demonstrated a clear signal in plasma cell populations of canine control tissues (lymph nodes, lymphoplasmacytic inflammation) showing a polyclonal pattern with a prevalence of λ-producing cells. CISH detected monotypic light chain expression in 33 of 53 (62%) PCTs, 31 expressing λ and 2 expressing к. CISH was more sensitive than IHC for λ light chain (58% vs. 47%, respectively) and more easily interpretable given the absence of confounding background staining. The absence of CISH staining for both λ and к in a considerable subset of tumors may be the result of lower light chain production by neoplastic cells. Multiple myeloma oncogene 1 (MUM1) was expressed by all but 2 PCTs (96%), which showed λ expression by CISH and IHC. The identification of poorly differentiated canine PCTs requires the assessment of a panel of IHC markers, with the potential support of CISH for Ig light chains.

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Determination of Clonality

Goto‐Koshino¹,

Tsujimoto²

2022

Schalm's Veterinary Hematology

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PCR-based clonality analysis of antigen receptor gene rearrangements in canine cutaneous plasmacytoma

Cited by 12 publications

References 19 publications

Plasma cell leukemia with plasmablastic morphology in a dog

Plasma cell leukemia with plasmablastic morphology in a dog

Chromogenic in situ hybridization for the detection of lambda and kappa immunoglobulin light chains as a potential auxiliary diagnostic technique in canine plasmacytomas

Determination of Clonality

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