PCR-based detection of chlamydial infection in swine and 
subsequent PCR-coupled genotyping of chlamydial <i>omp1</i>-gene 
amplicons by DNA-hybridization, RFLP-analysis, and 
nucleotide sequence analysis

Hoelzle, Ludwig E.; Steinhausen, G.; Wittenbrink, Max M.

doi:10.1017/s0950268899004446

Cited by 55 publications

(55 citation statements)

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“…abortus, Ch. pecorum, and C. suis were not detectable in Southern blotting studies using the entire ompA genes as a probe (Hoelzle et al, 2000). Although hitherto unspecified genus-specific antigenic sites responsible for cross-reactivity also reside in MOMP , the level of serological cross-reactivity between the rMOMPs as measured by ELISA did not exceed 17.2% of the corresponding homologous positive values.…”

Section: Discussionmentioning

confidence: 80%

Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera

Hoelzle

Wittenbrink

2004

Veterinary Microbiology

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Recombinant major outer membrane protein (MOMP) ofChlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera Abstract Recombinant major outer membrane proteins (rMOMP) of Chlamydophila (Ch.) abortus, Ch. pecorum, and Chlamydia (C.) suis were used as antigens to distinguish chlamydial species-specific antibodies in (i) immune sera from six rabbits and three pigs raised against native purified elementary bodies, (ii) serum samples from 25 sows vaccinated with Ch. abortus, and (iii) 40 serum samples from four heifers experimentally infected with Ch. abortus. All post-exposition sera contained chlamydial antibodies as confirmed by strong ELISA seroreactivities against the chlamydial LPS. For the rMOMP ELISA mean IgG antibody levels were at least 5.8-fold higher with the particular rMOMP homologous to the chlamydial species used for immunisation or infection than with heterologous rMOMPs (P <0.001).Preferential rMOMP ELISA reactivities of sera were confirmed by Western blotting. The results suggest that the entire chlamydial rMOMP could provide a species-specific serodiagnostic antigen.Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera AbstractRecombinant major outer membrane proteins (rMOMP) of Chlamydophila (Ch.) abortus, Ch. pecorum, and Chlamydia (C.) suis were used as antigens to distinguish chlamydial species-specific antibodies in (i) immune sera from six rabbits and three pigs raised against native purified elementary bodies, (ii) serum samples from 25 sows vaccinated with Ch. abortus, and (iii) 40 serum samples from four heifers experimentally infected with Ch. abortus. All post-exposition sera contained chlamydial antibodies as confirmed by strong ELISA seroreactivities against the chlamydial LPS. For the rMOMP ELISA mean IgG antibody levels were at least 5.8-fold higher with the particular rMOMP homologous to the chlamydial species used for immunisation or infection than with heterologous rMOMPs (P < 0.001). Preferential rMOMP ELISA reactivities of sera were confirmed by Western blotting. The results suggest that the entire chlamydial rMOMP could provide a species-specific serodiagnostic antigen. #

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Section: Discussionmentioning

confidence: 80%

Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera

Hoelzle

Wittenbrink

2004

Veterinary Microbiology

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“…the former porcine serovar of C. trachomatis, has been identified as the major agent causing chlamydioses in swine [22]. However, there appears to be a high prevalence of mixed infections with C. abortus making it difficult to attribute clinical pathology to C. suis alone [10]. In order to evaluate the aetiological importance of C. suis, an experimental challenge model of aerosol infection was established by our group [38].…”

Section: Introductionmentioning

confidence: 99%

An experimentally inducedChlamydiasuis infection in pigs results in severe lung function disorders and pulmonary inflammation

et al. 2008

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-This study was aimed at evaluating the pathophysiology of pulmonary dysfunctions and inflammatory consequences of an acute respiratory chlamydial infection induced experimentally in conventionally raised pigs (aged 39-44 days). Eight animals were exposed to Chlamydia suis (C. suis) and four non-infected animals served as controls. The total observation period was from seven days before challenge to seven days post exposure. While non-infected control pigs did not exhibit any clinical symptoms, animals exposed to C. suis developed fever and were severely respiratory distressed within the first week after exposure. After C. suis infection, pulmonary dysfunctions were characterised by a significant decrease in the diffusion capacity of the lung (i.e. transfer factor of the lung for carbon monoxide; TL CO), a significant increase in the functional residual capacity (FRC), and significant changes in the pattern of ventilation (respiratory rate increased while the tidal volume decreased). In exhaled breath condensate (EBC), leukotriene B 4 (LTB 4 ) and interleukin 6 (IL-6) showed a tendency to increase after infection. In the broncho-alveolar lavage fluid (BALF) of C. suis infected pigs, the activity of matrix metalloprotease 9 (MMP-9) was found to be increased compared to controls. BALF cytology was characterised by increased numbers of granulocytes and activated lymphocytes. Pulmonary inflammation in infected pigs was confirmed by post mortem histology. A prominent dissemination of chlamydial bodies in the lung was accompanied by an influx of macrophages, granulocytes and activated T-cells. Data obtained in this study provide new insight into the pathogenesis of acute respiratory chlamydial infections in pigs.Chlamydia suis model / pulmonary inflammation / lung functions / exhaled breath condensate (EBC) / BALF

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“…C. suis has been investigated in pigs in association with reproductive disorders, such as return to oestrus (with early embryonic death in >50% of sows) and inferior semen quality (with decreased sperm cell motility and death of >50% of sperm cells). These pathologies have been demonstrated on farms in Belgium, Cyprus, Estonia, Germany, Israel, and Switzerland [1,[12][13][14]. C. suis alone may be responsible for diarrhoea in young piglets, resulting in the partial loss of litters and increased owner costs incurred on veterinary care.…”

Section: Introductionmentioning

confidence: 99%

Novel locked nucleic acid (LNA)-based probe for the rapid identification of Chlamydia suis using real-time PCR

Lis

Kumala

Spalinski³

et al. 2014

BMC Vet Res

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Background: As the importance of chlamydial infections in pigs has become more obvious, a rapid and sensitive method to study the prevalence of Chlamydia suis in pig herds is required. Such a method should permit routine diagnostic tests for herds with clinical and subclinical infections, without the need for Chlamydia culture. Results:The main objective of this study was to develop a specific and rapid method for detecting C. suis in swine herds. A real-time PCR assay using a single locked nucleic acid (LNA)-containing probe specific for C. suis was developed based on the previously described 28S rDNA fragment used to identify Chlamydiales. Use of LNA nucleotides enabled the single probe to target a short, specific fragment of the 23S rRNA. The probe showed high specificity for C. suis and did not show any cross-reactivity with other Chlamydia or Chlamydophila species nor with swine DNA. All of the 86 tested field isolates, earlier identified as C. suis, were confirmed as positive using the newly developed assay. Conclusions: Using single LNA-based C. suis-specific probe allowed rapid and simple identification of this pathogen without requiring sequencing analysis and culturing. The proposed method may be used to study the prevalence of C. suis infection in pig herds and as a routine diagnostic test for herds with clinical and subclinical infection.

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PCR-based detection of chlamydial infection in swine and subsequent PCR-coupled genotyping of chlamydial omp1-gene amplicons by DNA-hybridization, RFLP-analysis, and nucleotide sequence analysis

Cited by 55 publications

References 36 publications

Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera

Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera

An experimentally inducedChlamydiasuis infection in pigs results in severe lung function disorders and pulmonary inflammation

Novel locked nucleic acid (LNA)-based probe for the rapid identification of Chlamydia suis using real-time PCR

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