PCR-based detection of Toxoplasma gondii from cattle in southern Iran

Molecular epidemiology of Toxoplasma gondii in cattle in Korea

“…This indicates that age is a risk factor for T. gondii infection because older individuals are exposed to the parasite for a longer duration than younger individuals [18]. With regard to sex, male cattle exhibited a significantly higher prevalence of T. gondii infection than female cattle in the current study, which is consistent with the findings of a previous study [14]. However, a higher prevalence in females was observed in another study [13].…”

“…Type II is the most prevalent genotype among humans [20]. In other studies, several types of T. gondii were identified in cattle, including types I (7.1%), II (70%), and III (17.2%) in Iran [14] and types I (1 sample) and II (4 samples) in Poland [13]. In the present study, 3 cattle tested positive for T. gondii, and using the B1 gene, we were able to identify the types I and/or III.…”

“…In Korea, T. gondii was detected in cattle in the Gyeongbuk Province using ELISA (0.5%, 3/568) in 2013 [5], in Ulleung Island using ELISA (17.6%, 97/552) between 2007-2010 [7], and in the eastern areas of the Gyeongbuk Province using ELISA (20.7%, 76/368) in 2008 [6]. Moreover, in Poland, T. gondii was detected in cattle using ELISA (13%, 313/2411) and in tissues using PCR (10.2%, 32/313) between 2009 and 2013 [13]; in Iran, it was detected in tissues us- ing PCR (56%, 70/125) in 2017 [14]. Blood PCR is typically more beneficial for the early and late diagnosis of toxoplasmosis [15]; however, only 0.7% of cattle in the present study were positive for toxoplasmosis.…”