PCR-based Identification of<i>Eubacteirum</i>species in endodontic infection

Kum, Kee‐Yeon; Fouad, Ashraf F.

doi:10.5395/jkacd.2003.28.3.241

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…It is well established that microbial infection of the root canal system is the cause of apical periodontitis [ 29 ]. Previous studies by 16S rRNA gene clone libraries demonstrated that 391 bacterial taxa belonging to 82 genera and 9 phyla have been identified in primary endodontic infections [ 30 – 33 ]. We used a culture-based approach to investigate the microbiota of primary endodontic infections with or without communications with the oral environment, along with further molecular identification by specific gene sequencing.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Propionibacterium acnes among the microbiota of primary endodontic infections with and without intraoral communication

et al. 2016

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ObjectivesThe presence of opportunistic pathogens such as Propionibacterium acnes (P. acnes) may contribute to the endodontic pathology. The presence of P. acnes may be influenced by different endodontic conditions.The aims of the study were firstly, to identify P. acnes within the whole cultivable microbiota of primary endodontic infections, to investigate which P. acnes phylotypes predominate in such infections and secondly to determine if the presence of an “open” communication (e.g. a sinus) can be associated with the isolation of P. acnes from the root canal.Material and methodsThe predominant cultivable microbiota of 15 primary endodontic lesions (7 without communication with the oral environment and 8 with an open communication) were identified using partial 16S ribosomal RNA (rRNA) gene sequence analysis. The identification of the organism was determined by interrogating the Human Oral Microbiome Database. The P. acnes isolates were typed on the basis of the recA gene sequence comparison. A neighbor-joining tree was constructed using MEGA 4.1 with the inclusion of known recA sequences.ResultsThere was no difference in the number of species identified from lesions without communication (5.86 ± 3.7) and those with communication (5.37 ± 3.6) (P > 0.05). PCR-based 16S rRNA gene sequencing revealed P. acnes as the most prevalent isolate recovered from lesions with communication. recA gene sequencing revealed two phylogenetic lineages present in lesion with communication, with mainly type I (further split into type IA and type IB) and type II.ConclusionsThe presence of P. acnes as opportunistic pathogens has been confirmed and may sustain the traits observed in specific clinical presentations.Clinical relevanceClinical management of open lesions may require further disinfection to eliminate opportunistic bacteria.

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Section: Discussionmentioning

confidence: 99%

Isolation of Propionibacterium acnes among the microbiota of primary endodontic infections with and without intraoral communication

et al. 2016

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“…. 따라서 치주염과 같은 상황이 일어나면 치은열 구나 치주낭 혹은 치근단 경로를 통해 치수에 영향을 주게 되고 21,22) , [34][35][36] . 지금까지 당뇨 및 비당뇨 환자에서 치주질환과 관련된 미생물 분포 패턴에 대한 연구들은 많이 이루어져왔지만 37,38) , 당뇨와 특이 근관 감염균과의 상관성 에 대한 연구는 아직 미비하다.…”

Section: The Role Of Type 2 Diabetes As a Predisposing Risk Factor Onunclassified

The role of Type 2 Diabetes as a predisposing risk factor on the pulpo-periapical pathogenesis: review article

Kim

Bae

Seo

et al. 2009

J Korean Acad Conserv Dent

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Diabetes Mellitus (DM) is a syndrome accompanied with the abnormal secretion or function of insulin, a hormone that plays a vital role in controlling the blood glucose level (BGL). Type 1and 2 DM are most com mon form and the prevalence of the latter is recently increasing. The aim of this article was to assess whet her Type 2 DM could act as a predisposing risk factor on the pulpo-periapical pathogenesis. Previous literature on the pathologic changes of blood vessels in DM was thoroughly reviewed. Furthermore, a histopath ologic analysis of artificially-induced periapical specimens obtained from Type 2 diabetic and DM-resistant rats was compared. Histopathologic results demonstrate that the size of periapical bone destruction w as larger and the degree of pulpal inflammation was more severe in diabetic rats, indicating that Type 2 D M itself can be a predisposing risk factor that makes the host more susceptible to pulpal infection. The pos sible reasons may be that in diabetic state the lumen of pulpal blood vessels are thickened by atheromatous deposits, and microcirculation is hindered. The function of polymorphonuclear leukocyte is also impair ed and the migration of immune cells is blocked, leading to increased chance of pulpal infection. Also, lack of collateral circulation of pulpal blood vessels makes the pulp more susceptible to infection. These decrease the regeneration capacity of pulpal cells or tissues, delaying the healing process. Therefore, when restorative treatment is needed in Type 2 DM patients, dentists should minimize irritation to the pulpal tissue un der control of BGL. [J Kor Acad Cons Dent 34(3):169-176, 2009]

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Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing

Lim

Lee

Kim

et al. 2011

J Korean Acad Conserv Dent

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PCR-based Identification ofEubacteirumspecies in endodontic infection

Cited by 3 publications

References 26 publications

Isolation of Propionibacterium acnes among the microbiota of primary endodontic infections with and without intraoral communication

Isolation of Propionibacterium acnes among the microbiota of primary endodontic infections with and without intraoral communication

The role of Type 2 Diabetes as a predisposing risk factor on the pulpo-periapical pathogenesis: review article

Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing

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