PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant

Abstract

Nepenthes khasiana Hook. f. belonging to monotypic family Nepenthaceae is a rare, endangered, dioecious member of the carnivorous plant found in North-East India. The plant is endemic to the Indian state of Meghalaya and is distributed throughout the state from West Khasi hills to East Khasi hills, Jaintia hills and East, West to South Garo hills from 1,000 to ca. 1,500 m altitude. Multi-locus analysis using PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers were used for the first time to assess the genetic diversities of N. khasiana Hook. f. collected from different parts of Meghalaya. It was observed that RAPD analysis showed more polymorphism than ISSR fingerprinting in revealing genetic polymorphism in N. khasiana Hook. f. The result of cluster analysis by using UPGMA method showed that the groups based on pooled RAPD-ISSR genetic similarity were more similar than the groups based on RAPD. Furthermore, genetic similarity reveals variability within the population at Jarain of Jaintia hills, while between populations the Baghmara region differs from the others with at least 40% dissimilarity. The results show a broad range of genetic diversity within the populations of N. khasiana Hook. f.

A molecular genetics of Drosera spatulata complex by using of RAPD analysis

“…Carnivorous plants are interesting botanical oddities that show a range of characters different from those of a normal plant (Bhau et al 2009). These plants typically inhabit environments where nutrients are limited, and insects trapping may have evolved as a means of providing a supplemental source of nutrients, especially nitrogen (Givnish et al 1984;Gallie and Chang 1997;Bhau et al 2009).…”

“…These plants typically inhabit environments where nutrients are limited, and insects trapping may have evolved as a means of providing a supplemental source of nutrients, especially nitrogen (Givnish et al 1984;Gallie and Chang 1997;Bhau et al 2009). Then, carnivorous plants have long attracted the attention of botanists, because of their highly specialized morphology and curious trapping mechanisms (Juniper et al 1989;Rivadavia et al 2003).…”

Biodiversity Conservation with Special Reference to Medicinal Climbers: Present Scenario, Challenges, Strategies, and Policies

“…Due to its attractive beauty, this plant has attracted horticultural interes t (Mukerjee et al 1984 ;Khoshbakht and Hammer 2007 ). The plant's existence is threatened because of its collection and export by the local plant collectors to other states of India on account of the fascinating beauty of its pitcher (Bhau et al 2009 ). The species has been classifi ed as a threatened species and is included in the list of rare and threatened taxa of India (Jain and Baishya 1977 ;Jain and Sastri 1980 ).…”

RAPD Profiling of Three Japanese <i>Drosera</i> Species

“…Carnivorous plants are a group with highly specialized morphology for insect trapping, and thus appeal to botanists (Juniper et al 1989, Rivadavia et al 2003, Bhau et al 2009, Hoshi et al 2010. The Droseraceae, which is a representative family of carnivorous plants, contains three genera of Aldrovanda, Dionaea and Drosera (Takahashi and Sohma 1982, Juniper et al 1989, Conran et al 1997, Williams et al 1994, Rivadavia et al 2003.…”