PCR Detection of Listeria monocytogenes in different Food Products compared with the mini-VIDAS LMO System and the Standard Procedure ISO 11290-1

Aznar, Rosa; Solís, Inmaculada

doi:10.1007/s00003-006-0019-0

Cited by 8 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, mini-VIDAS was less sensitive and also had lower PPV and NPV scores. This is in accordance with the results of an earlier work (Aznar & Solís, 2006) in which the PCR procedure described by Aznar and Alarcón (2003) proved more sensitive in L. monocytogenes detection than the mini-VIDAS, in a variety of naturally contaminated samples. Overall, the results obtained in this study for L. monocytogenes and E. coli O157:H7, using a high number of naturally contaminated food products, highlighted that qPCR without enrichment is a better predictive tool than mini-VIDAS, which corroborates the results obtained for Salmonella spp.…”

Section: Discussionsupporting

confidence: 93%

Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis

2011

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 93%

Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis

2011

Self Cite

View full text Add to dashboard Cite

“…The comparison of the two sample evaluation methods revealed that RT-PCR is 2.71 to 9.40 times more sensitive than the culture method ( Table 3). The higher sensitivity of PCR methods has been reported (4,9,30,44,45), including for raw milk samples in Italy (1,5,19,20). The fact that the RT-PCR method detects dead cells should be taken into account in comparing the sensitivity of the two methods, but this difference has no impact on the development of a risk assessment of consumption of raw milk sold in vending machines.…”

Section: Discussionmentioning

confidence: 99%

Four-Year Monitoring of Foodborne Pathogens in Raw Milk Sold by Vending Machines in Italy

Giacometti

Bonilauri

Serraino

et al. 2013

Journal of Food Protection

View full text Add to dashboard Cite

Prevalence data were collected from official microbiological records monitoring four selected foodborne pathogens (Salmonella, Listeria monocytogenes, Escherichia coli O157:H7, and Campylobacter jejuni) in raw milk sold by self-service vending machines in seven Italian regions (60,907 samples from 1,239 vending machines) from 2008 to 2011. Data from samples analyzed by both culture-based and real-time PCR methods were collected in one region. One hundred raw milk consumers in four regions were interviewed while purchasing raw milk from vending machines. One hundred seventy-eight of 60,907 samples were positive for one of the four foodborne pathogens investigated: 18 samples were positive for Salmonella, 83 for L. monocytogenes, 24 for E. coli O157:H7, and 53 for C. jejuni in the seven regions investigated. No significant differences in prevalence were found among regions, but a significant increase in C. jejuni prevalence was observed over the years of the study. A comparison of the two analysis methods revealed that real-time PCR was 2.71 to 9.40 times more sensitive than the culture-based method. Data on consumer habits revealed that some behaviors may enhance the risk of infection linked to raw milk consumption: 37% of consumers did not boil milk before consumption, 93% never used an insulated bag to transport raw milk home, and raw milk was consumed by children younger than 5 years of age. These results emphasize that end-product controls alone are not sufficient to guarantee an adequate level of consumer protection. The beta distribution of positive samples in this study and the data on raw milk consumer habits will be useful for the development of a national quantitative risk assessment of Salmonella, L. monocytogenes, E. coli O157, and C. jejuni infection associated with raw milk consumption.

show abstract

“…This retrospective study confirms the well-reported higher sensitivity of the RT-PCR versus cultural method (Karns et al ., 2005 ; Chapman et al ., 2001 ; Yang et al ., 2003 ; Aznar and Solis, 2006 ; Wegmüller et al ., 1993 ); in fact, our results show that, in spite of the use of the same enrichment broth, the RT-PCR method disclosed a percentage of positive samples that were negative to cultural examination ranging between 20 and 43%, with a PCR/culture ratio between 2.37 to 5.00.…”

Section: Discussionmentioning

confidence: 99%

Detection of food hazards in foods: comparison of real time polymerase chain reaction and cultural methods

Bonilauri¹,

Bardasi²,

Leonelli³

et al. 2016

Ital J Food Safety

View full text Add to dashboard Cite

Foodstuffs should not contain microorganisms or their toxins or metabolites in quantities suggesting an unacceptable risk for human health. The detection of food hazards in foods is performed by several tests that produce results dependent on the analytical method used: an analytical reference method, defined as standard, is associated with each microbiological criterion laid down in Regulation 2073/2005/EC, but, analytical methods other than the reference ones, in particular more rapid methods, could be used. Combined screening methods performed by real time-polymerase chain reaction (RT-PCR) are currently validated as alternative methods according to the ISO 16140:2003 and certified by the Association Française de Normalisation. However, the positive results obtained with these alternative methods, the investigated molecular relations that resulted positive have to be confirmed with cultural methods using the same enrichment media in which the molecular screening was performed. Since it is necessary to assess if these testing schemes provide equivalent guarantees of food safety, the aim of this retrospective study is to analyse the data collected, from 2012 to 2014 by Emilia Romagna Region in the field of Piano Regionale Alimenti (Food Regional Plan) during official controls monitoring food samples of animal and other than animal origin. Records performed by combined methods of molecular screening of Salmonella spp., Listeria monocytogenes and thermophilic Campylobacter and cultural confirmation results were gathered together and the results were compared in order to assess the sensitivity of the methods. A total of 10,604 food samples were considered in this study: the comparison of the data revealed that the RT-PCR method detected Salmonella, L. monocytogenes, and thermophilic Campylobacter in 2.18, 3.85 and 3.73% of the samples, respectively, whereas by using cultural method these pathogens were isolated in 0.43, 1.57 and 1.57% of samples, respectively. In spite of the use of the same enrichment broth, the RT-PCR method disclosed a percentage of positive samples that was negative to cultural examination ranging between 20 and 43%, with a PCR/culture ratio between 2.37 to 5.00. In conclusion, the results of this study pose a doubt about the sensitivity of the official cultural methods regarding the isolation of the three investigated foodborne pathogens. Moreover this study may be a useful tool for veterinary authorities to assess appropriate sampling plans to control the risk relating to the consumption of contaminated foods.

show abstract

PCR Detection of Listeria monocytogenes in different Food Products compared with the mini-VIDAS LMO System and the Standard Procedure ISO 11290-1

Cited by 8 publications

References 26 publications

Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis

Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis

Four-Year Monitoring of Foodborne Pathogens in Raw Milk Sold by Vending Machines in Italy

Detection of food hazards in foods: comparison of real time polymerase chain reaction and cultural methods

Contact Info

Product

Resources

About