PCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA

Abstract

Background and Purpose:The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. Materials and Methods:In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fusarium species, PCR analysis was performed for the amplification of ITS regions. Afterwards, the location of the effective endonuclease enzymes has been evaluated within approximately 930 bp of rDNA sequence. Results:Through the selected enzymes including; HhaI, MspI, TaqI and FaqI, the mentioned Fusarium species have been divided into 33 groups. The first three enzymes were able to classify Fusarium species into 23 groups of which 19 groups included one member, one group included two members and three groups included three members of the Fusarium species. This study also revealed the possibility in the identification of F. semitectum, F. solani complex, F. pseudograminearum, F. nisikadoi, F. coeruleum and F. acuminatum species by one unique enzyme. In addition, our study indicated the ability of the differentiation of F. Compactum from F. equiseti.Conclusion:As Compared to previous studies with more endonuclease enzymes and with limited in identifications, the ITS-RFLP patterns reported here an attempted to evaluate most of the Fusarium species successfully.

Fusarium spp. Mycotoxin Production, Diseases and their Management: An Overview

“…Different molecular techniques which include Random Amplified Polymorphic DNA (RAPD) analysis, specific diagnostic PCR primers and DNA sequencing are being used to identify Fusarium species. The polymerase chain reaction (PCR) is considered (Mulè et al, 2004) as the most reliable and rapid technique for the identification of different Fusarium species (Kachuei et al, 2015). Different primer sets have been designed for the identification of Fusarium species (Table 3).…”

DNA Marker Identification of Trichoderma and Fusarium Level Species

“…Tef nucleotide sequence is a powerful marker to show association among fungi at all level due to highly informative at species level [10] . Fusarium taxa is one of the main fungal sorts that connected with the specialty of medicine, veterinary and agricultural [11] The pathogenicity of the species leads to Fusarium head blight (FHB) in wheat and many cereals that decrease the mass product by 30-70% [12]. The fungus Fusarium are well studied in different areas like ecology, plant pathology, medicalmycology and toxicology because it is one of the main ungus a s c a u s a t i v e a g e n t a s phytopathogen, cause infection to human and secrete mycotoxins that linked to human being and animal [13].…”

Journal of Plant Protection Research

“…Fusarium head blight (FHB) is one of the most dangerous diseases of wheat, barley, rye, oat, triticale and corn contributing to economic losses (Fernandez et al 2005;Dweba et al 2017). There are methods of molecular qualitative detection of fungal colonies grown on solid media (Divakara et al 2013) including polymerase chain reaction (PCR), random amplified polymorphic DNA-PCR (RAPD-PCR) and restriction fragment length polymorphism-PCR (RFLP--PCR) (Kachuei et al 2015) but it takes a few days to let them grow, delaying the analytical process. However, PCR based methods are also currently being used for pathogen identification from environmental samples.…”