pCRP-mCRP Dissociation Mechanisms as Potential Targets for the Development of Small-Molecule Anti-Inflammatory Chemotherapeutics

Caprio, Vittorio; Badimon, Lina; Napoli, Mario Di; Fang, Wen; Ferris, Glenn; Guo, Baoqiang; Iemma, Rocco S.; Liu, Donghui; Zeinolabediny, Yasmin; Slevin, Mark

doi:10.3389/fimmu.2018.01089

Cited by 38 publications

(51 citation statements)

References 83 publications

(84 reference statements)

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“…mCRP activates platelets [20], monocytes [44], and endothelial cells [45]. It is a key substance that promotes in ammatory response [21,46]. The mechanism underlying the in ammatory response of endothelial cells is caused by the interaction of mCRP with neutrophils, macrophages, and platelets [46,47].…”

Section: Discussionmentioning

confidence: 99%

“…It is a key substance that promotes in ammatory response [21,46]. The mechanism underlying the in ammatory response of endothelial cells is caused by the interaction of mCRP with neutrophils, macrophages, and platelets [46,47]. Furthermore, mCRP binds to CFH and directs it to the damaged part of the cell [48,49], inactivates C3b, and limits the progression of in ammation [39].…”

Section: Discussionmentioning

confidence: 99%

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Plasma level of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody-associated vasculitis

Wu¹,

Liang

Huang³

et al. 2020

Preprint

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[Background] C-reactive protein (CRP) has two natural isomers: C-reactive protein pentamer (pCRP) and C-reactive protein monomer (mCRP). The levels of CRP are significantly elevated in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). mCRP not only activates the endothelial cells, platelets, leukocytes, and complements, but also has a proinflammatory structural subtype that can localize and deposit in inflammatory tissues. Thus, it regulates a variety of clinical diseases, such as ischemia/reperfusion (I/R) injury, Alzheimer’s disease, age-related macular degeneration, and cardiovascular disease. We hypothesized that plasma mCRP levels are related to cardiovascular disease in AAV. [Methods] In this cross-sectional study, 37 patients with AAV were assessed. Brain natriuretic peptide (BNP) and mCRP in plasma were assessed by enzyme-linked immunosorbent assay (ELISA). The acute ST-segment elevation myocardial infarction (STEMI) was diagnosed by coronary angiography, and the Gensini score calculated. Echocardiography evaluated the ejection fraction (EF%), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), and left ventricular mass index (LVMI). Estimated glomerular filtration rate (eGFR) was calculated based on serum creatinine, age, and gender. [Results] The plasma level of mCRP in AAV was significantly higher than that in healthy volunteers (P<0.001). Then, mCRP and CRP levels were compared with and without STEMI complications in AAV. The plasma level of mCRP was higher, but that of CRP was lower in STEMI. The plasma level of mCRP was correlated with Birmingham vasculitis activity score (BVAS), eGFR, BNP, EF%, LVEDV, LVESV, LVMI, and STEMI complications’ Gensini score in AAV; however, CRP did not correlate with BNP, EF%, LVEDV, LVESV, LVMI, and Gensini score.[Conclusions] The plasma level of mCRP was related to cardiovascular diseases in AAV patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Plasma level of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody-associated vasculitis

Wu¹,

Liang

Huang³

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…mCRP can activate platelets 22 , monocytes 45 and endothelial cells 46 . mCRP is a key substance that promotes inflammatory response 23,47 . The inflammatory response mechanism of endothelial cells is caused by the interaction of mCRP with neutrophils, macrophages, and platelets 47,48 .…”

Section: Discussionmentioning

confidence: 99%

“…mCRP is a key substance that promotes inflammatory response 23,47 . The inflammatory response mechanism of endothelial cells is caused by the interaction of mCRP with neutrophils, macrophages, and platelets 47,48 . mCRP induces IL-8, MCP-1, E-selectin, ICAM-1, VCAM-1 and VCAM-1 in endothelial cells leading to increased adhesion of neutrophils 47,49 .…”

Section: Discussionmentioning

confidence: 99%

“…The inflammatory response mechanism of endothelial cells is caused by the interaction of mCRP with neutrophils, macrophages, and platelets 47,48 . mCRP induces IL-8, MCP-1, E-selectin, ICAM-1, VCAM-1 and VCAM-1 in endothelial cells leading to increased adhesion of neutrophils 47,49 . mCRP can bind to FH and direct FH to the damaged part of the cell 50,51 , inactivate C3b, and limit the progression of inflammation 40 .…”

Section: Discussionmentioning

confidence: 99%

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The plasma levels of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody -associated vasculitis

Wu¹,

Liang

Ding³

et al. 2020

Preprint

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Background C-reactive protein (CRP) has 2 natural isomers, C-reactive protein pentamer (pCRP) and C-reactive protein monomer (mCRP). The levels of CRP are significantly elevated in patients with anti-neutrophil cytoplasmic antibody（ANCA)-associated vasculitis (AAV). mCRP not only can cause the activation of endothelial cells, platelets, leukocytes, and complements, but also have a proinflammatory structural subtype that can localise and deposit in inflammatory tissues, so it is an important mediator of a variety of clinical diseases, such as Ischemia/Reis perfusion (I/R) injury, Alzheimer's disease, age-related macular degeneration, and cardiovascular disease. Complications of myocardial infarction occurred（n=4) in 37 AAV patients we collected, so it is speculated that mCRP may be related to cardiovascular disease in patients. Methods Brain natriuretic peptide (BNP) and mCRP in plasma were tested by enzyme-linked immunosorbent assay (ELISA). The Acute ST-segment elevation myocardial infarction（STEMI) diagnosed by Coronary angiography and then calculated the Gensini score. Echocardiography: ejection fraction （EF%)，left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular mass index (LVMI). Glomerular filtration rate (eGFR) was calculated based on serum creatinine, age, and gender. Results The plasma levels of mCRP in AAV was significantly higher than that in healthy volunteers (P<0.001). There were 4 STEMI complications in AAV. We compared mCRP and CRP levels with STEMI complication and without STEMI complications in AAV, the plasma levels of mCRP was higher, however the plasma levels of CRP was lower in STEMI. Plasma levels of mCRP correlated with Birmingham vasculitis activity score (BVAS), eGFR, BNP, EF%, LVEDV, LVESV, LVMI ( r =0.404, P =0.013; r =-0.341, P =0.039; r =0.349, P =0.034; r =-0.362, P =0.027; r =0.375, P =0.022; r =0.334, P =0.043; r =0.736, P <0.001) and STEMI complications’ Gensini score (r=0.977, P=0.023) in AAV, however CRP didn’t correlate with BNP, EF%, LVEDV, LVESV, LVMI and Gensini score. Conclusions The plasma levels of mCRP related to cardiovascular diseases in AAV patients.

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Photonic sensor to detect rapid changes in CRP levels

Jędrzejewska‐Szczerska

Kosowska

Viter

et al. 2022

Journal of Biophotonics

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One of the most important biomarkers used to determine inflammation is C‐reactive protein (CRP). Its level, when it is within the range that does not define inflammation, informs about the risk of cardiovascular events. If the norm is exceeded and inflammation is detected in the body, CRP level can increase 1000 times within a few hours. The type of infection can also be determined based on the level of elevated CRP. All this makes CRP a very important element of diagnostics. A sensor based on low coherence interference is presented. Preliminary studies have shown that its sensitivity is 5.65 μg/L and the measurement time is short, <10 min. The entire system is built of commercially available components, which allow production cost minimalization. In addition, the user‐friendly operation allows it to be operated by unqualified people. Due to these features, our solution is a promising alternative to commercially used enzyme‐linked immunosorbent assay, which needs trained personnel to perform time‐consuming measurement procedures.

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pCRP-mCRP Dissociation Mechanisms as Potential Targets for the Development of Small-Molecule Anti-Inflammatory Chemotherapeutics

Cited by 38 publications

References 83 publications

Plasma level of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody-associated vasculitis

Plasma level of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody-associated vasculitis

The plasma levels of mCRP is linked to cardiovascular disease in antineutrophil cytoplasmic antibody -associated vasculitis

Photonic sensor to detect rapid changes in CRP levels

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