pDB2011, a 7.6kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts

Bertsch, David; Anderegg, Janine; Lacroix, Christophe; Meile, Léo; Stevens, Marc J. A.

doi:10.1016/j.plasmid.2013.06.001

Cited by 22 publications

(15 citation statements)

References 17 publications

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“…In conclusion, the relatively low number of AB R in all Listeria (3.1%) and L. monocytogenes strains (1.8%) from this work is in agreement with previous studies from Europe and does not implicate an apparent increase of AB R in this genus in this decade. However, next to the relatively common tet(M) gene, more detailed parallel studies revealed the presence of a presumably novel resistance to clindamycin (Bertsch et al 2013a), of the novel conjugative transposon Tn6198 (Bertsch et al 2013b), and of the novel, broad host-range, multidrug resistance plasmid pDB2011 (Bertsch et al 2013c) among the 524 Listeria strains analyzed during this study. Even though no genes conferring resistance to ampicillin were detected in this study, the elevated MIC values of 4 lg mL À1 might warrant an increase in dosages for the successful treatment of listeriosis.…”

Section: Horizontal Gene Transfer Potentialmentioning

confidence: 78%

“…The genetic basis of the novel transposon Tn 6198 encoding tet (M) and dfrG , which was isolated from the clinical L. monocytogenes isolate TTH‐2007, and of the novel plasmid pDB2011 carrying dfrD , erm (A), and spc , detected in the foodborne L. innocua strain TTS‐2011, was published by our group elsewhere (Bertsch et al. ,c). Acquired multidrug resistance seems to be exceptional in Listeria (Morvan et al.…”

Section: Resultsmentioning

confidence: 99%

“… 2013b ), and of the novel, broad host-range, multidrug resistance plasmid pDB2011 (Bertsch et al. 2013c ) among the 524 Listeria strains analyzed during this study. Even though no genes conferring resistance to ampicillin were detected in this study, the elevated MIC values of 4 μ g mL −1 might warrant an increase in dosages for the successful treatment of listeriosis.…”

Section: Resultsmentioning

confidence: 99%

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Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes

et al. 2014

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The aims of this study were to assess antibiotic resistance pheno-and genotypes in foodborne, clinical, and environmental Listeria isolates, as well as to elucidate the horizontal gene transfer potential of detected resistance genes. A small fraction of in total 524 Listeria spp. isolates (3.1%) displayed acquired antibiotic resistance mainly to tetracycline (n = 11), but also to clindamycin (n = 4) and trimethoprim (n = 3), which was genotypically confirmed. In two cases, a tetracycline resistance phenotype was observed together with a trimethoprim resistance phenotype, namely in a clinical L. monocytogenes strain and in a foodborne L. innocua isolate. Depending on the applied guidelines, a differing number of isolates (n = 2 or n = 20) showed values for ampicillin that are on the edge between intermediate susceptibility and resistance. Transferability of the antibiotic resistance genes from the Listeria donors, elucidated in vitro by filter matings, was demonstrated for genes located on transposons of the Tn916 family and for an unknown clindamycin resistance determinant. Transfer rates of up to 10−5 transconjugants per donor were obtained with a L. monocytogenes recipient and up to 10−7 with an Enterococcus faecalis recipient, respectively. Although the prevalence of acquired antibiotic resistance in Listeria isolates from this study was rather low, the transferability of these resistances enables further spread in the future. This endorses the importance of surveillance of L. monocytogenes and other Listeria spp. in terms of antibiotic susceptibility.

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Section: Horizontal Gene Transfer Potentialmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes

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“…FN377602) (61) and pDB2011 from Listeria innocua (GenBank accession no. KC456362) (62). Both the PV-01 and p1128105 cfr environments contain the ORF1-cfr-rec-hp-pre/mob gene insertion within identical rep gene sequences; however, the rep gene is disrupted in different locations.…”

Section: Resultsmentioning

confidence: 99%

Linezolid-Resistant Staphylococcus aureus Strain 1128105, the First Known Clinical Isolate Possessing thecfrMultidrug Resistance Gene

Locke

Zuill

Scharn

et al. 2014

Antimicrob Agents Chemother

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The Cfr methyltransferase confers resistance to six classes of drugs which target the peptidyl transferase center of the 50S ribosomal subunit, including some oxazolidinones, such as linezolid ( L inezolid (LZD) resistance occurs predominantly through structural alterations to the oxazolidinone binding site in the 50S peptidyl transferase center (PTC) (1). These conformational changes are the result of mutations in genes encoding 23S rRNA (2, 3) or the ribosomal proteins L3 and L4 (4, 5) or via posttranscriptional modification of 23S rRNA base A2503 by the Cfr methyltransferase (6). Highly LZD r isolates that possess both the cfr gene and chromosomally encoded mutations have been identified (7-10).The horizontally transferrable, plasmid-borne nature of cfr makes this resistance determinant inherently more worrisome than chromosomally encoded resistance mechanisms that must arise independently and/or disseminate clonally (11,12). Adding to the potential for spread is the low fitness cost of this gene (13) and the broad spectrum of resistance conferred by Cfr to drugs included in the PhLOPS A phenotype (phenicol, lincosamide, oxazolidinone, pleuromutilin, and streptogramin A class antibiotics) (6,12,14), as well as 16-member-ring macrolides (15). Within the oxazolidinone class, there are differences in susceptibilities of strains possessing cfr depending on structural features at both ends of the molecule. The addition of D-ring systems that pick up additional binding interactions in the PTC and the substitution of an A-ring C-5 hydroxymethyl group (in place of the bulkier acetamide-containing substituent found on LZD and other oxazolidinones) allow oxazolidinones such as tedizolid (TZD) (16) to retain greater potency than LZD in the presence of Cfr methylation (17).The cfr gene was first identified on the pSCFS1 plasmid in a Staphylococcus sciuri isolate recovered from a florfenicol-treated calf in Bavaria in 1997 (18). Since then, a variety of other veterinary staphylococci possessing cfr-bearing plasmids sharing similarity either to pSCFS1 or to one of two other groups possessing regions containing the cfr gene flanked by either IS256 or IS21-558 mobile elements have been identified (19). The first clinical cfrpositive strains reported were Staphylococcus aureus isolates recovered in 2005 from Ireland and Colombia. Irish methicillinresistant S. aureus (MRSA) isolate M05/0060 (no specific collection date given) (20) possessed the cfr-bearing pSCFS7 plasmid, which has similarity to the IS21-558-carrying veterinary plasmid pSCFS3 (21), although only a truncated portion of the IS21-558 element remained (22). Colombian MRSA isolate CM05 was recovered in May 2005 from a patient in Medellin, Colombia, who had briefly undergone LZD therapy (2 doses) (6, 23). The cfr gene in CM05 was chromosomally located and shared flanking sequence with high similarity to the pSM19035 Gram-positive multidrug resistance plasmid, including an IS21-558 element (24,25). Subsequently, additional cfr-positive clinical staphylococci and ente...

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“…O gene tet(M) é carreado, frequentemente, por elementos genéticos móveis, como os transposons conjugativos da família Tn916-1545 comumente encontrados em Enterococcus spp. e que podem ser transferidos entre as diferentes espécies de bactérias Gram positivas, incluindo Listeria spp.Testes realizados in vitro ou em matrizes alimentares demonstraram a transferência do gene tet(M) entre diferentes espécies de Enterococcus e L. innocua(RIZZOTTI et al, 2009), entre L. monocyogenes e Enterococcus faecalis(BERTSCH et al, 2013), entre bactérias ácido láticas e Listeria spp (TOOMEY et al, 2009(POURSHABAN et al, 2002…”

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Caracterização fenotípica e genotípica de Listeria monocytogenes isoladas de produtos cárneos crus comercializados no município de São Paulo

Rowlands¹

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Ao meu marido Alexandre, pelo amor, carinho, apoio , companherismo e paciência. A minha princesinha Isabella que chegou no meio deste doutorado para encher meu coração de alegria e dar muito mais sentido a minha vida. Aos meus queridos pais, João e Aparecida, pelo amor, dedição, carinho e por sempre me apoiarem. Aos meus irmãos, Rosangela e João Roberto, companheiros para todos os momentos. Aos meus queridos sobrinhos Matheus e João Victor.

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pDB2011, a 7.6kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts

Cited by 22 publications

References 17 publications

Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes

Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes

Linezolid-Resistant Staphylococcus aureus Strain 1128105, the First Known Clinical Isolate Possessing thecfrMultidrug Resistance Gene

Caracterização fenotípica e genotípica de Listeria monocytogenes isoladas de produtos cárneos crus comercializados no município de São Paulo

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