PDGF-BB Carried by Endothelial Cell–Derived Extracellular Vesicles Reduces Vascular Smooth Muscle Cell Apoptosis in Diabetes

Togliatto, Gabriele; Dentelli, Patrizia; Rosso, Arturo; Lombardo, Giusy; Gili, Maddalena; Gallo, Sara; Gai, Chiara; Solini, Anna; Camussi, Giovanni; Brizzi, Maria Felice

doi:10.2337/db17-0371

Cited by 46 publications

(27 citation statements)

References 55 publications

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“…However, in our experimental setting, VSMCs acted as recipients in the perivascular microenvironment to accept miR‐221‐3p containing EVs. In recent reports, VSMCs could internalize EVs derived from endothelial cells (49) or macrophages (14). However, the exact loading mechanism of miR‐221‐3p into EVs was not addressed in this study and remains to be explored.…”

Section: Discussionmentioning

confidence: 99%

Perivascular adipose tissue–derived extracellular vesicle miR‐221‐3p mediates vascular remodeling

Ballantyne²,

et al. 2019

The FASEB Journal

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Adipose tissue–secreted extracellular vesicles (EVs) containing microRNAs (miRNAs) convey intercellular message signaling. The biogenesis of EV‐miRNAs from perivascular adipose tissue (PVAT) and their roles in intercellular communication in response to obesity‐associated inflammation have not yet been fully explored. By feeding mice a high‐fat diet for 16 wk, we established obesity‐associated, chronic low‐grade inflammation in PVAT, characterized as hypertrophy of perivascular adipocytes, decreased adipogenesis, and proinflammatory macrophage infiltration. We show that PVAT‐derived EVs and their encapsulated miRNAs can be taken up into vascular smooth muscle cells (VSMCs) in vivo and in vitro. miR‐221‐3p is one of the highly enriched miRNAs in obese PVAT and PVAT‐derived EVs. Transfer and direct overexpression of miR‐221‐3p dramatically enhances VSMC proliferation and migration. Peroxisome proliferator–activated receptor γ coactivator 1α is identified as a miR‐221‐3p target in VSMC phenotypic modulation. Obese mice secrete abundant miRNA‐containing EVs, evoking inflammatory responses in PVAT and vascular phenotypic switching in abdominal aorta of lean mice. Local delivery of miR‐221‐3p mimic in femoral artery causes vascular dysfunction by suppressing the contractile genes in the arterial wall. Our findings provide an EV–miR‐221‐3p–mediated mechanism by which PVAT triggers an early‐stage vascular remodeling in the context of obesity‐associated inflammation.—Li, X., Ballantyne, L. L., Yu, Y., Funk, C. D. Perivascular adipose tissue–derived extracellular vesicle miR‐221‐3p mediates vascular remodeling. FASEB J. 33, 12704–12722 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

Perivascular adipose tissue–derived extracellular vesicle miR‐221‐3p mediates vascular remodeling

Ballantyne²,

et al. 2019

The FASEB Journal

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“…IGF‐1, as a growth factor, has the ability to reduce the apoptosis of periodontal ligament fibroblasts through participation in several signalling pathways 25 . In addition, TGF‐β, VEGF, PDGF and bFGF also influence the inhibition of apoptosis 26–28 …”

Section: Discussionmentioning

confidence: 99%

“…25 In addition, TGF-β, VEGF, PDGF and bFGF also influence the inhibition of apoptosis. [26][27][28] The showed that the viability of PDL cells is decreased after storage for 6, 24, 48 and 72 hours in sterile HBSS at 37°C. 30 Souza et al found that the storage time in HBSS has a negative influence on its ability to promote the proliferation of periodontal ligament fibroblasts, and it is suspected that HBSS storage may cause an alteration in the concentration of its components, making them insufficient to nourish the cells for more than 6 hours.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of conditioned medium from placenta‐derived mesenchymal stem cells as a storage medium for avulsed teeth: An in vitro study

Jiang

et al. 2020

Dental Traumatology

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Background/Aim: The viability of periodontal ligament cells on the root surface is a major factor that influences the healing of replanted teeth. A suitable storage medium is necessary to preserve avulsed teeth before replantation. Conditioned medium from placenta-derived mesenchymal stem cells (PMSC-CM) contains a variety of growth factors. The aim of this study was to evaluate the effectiveness of PMSC-CM as a storage medium to maintain the cell viability of avulsed teeth. Material and Methods: Extracted premolars from healthy humans were randomly stored in Hank's balanced salt solution (HBSS) and PMSC-CM for 6, 12 and 24 hours, respectively, at room temperature, and then the ratio of apoptosis of the periodontal ligament (PDL) cells was identified by flow cytometry. Human periodontal ligament stem cells (PDLSCs) were incubated with HBSS and PMSC-CM, respectively, for 6, 12, 24 and 48 hours in 5% CO 2 at 37°C. Then, the cell viability of PDLSCs was determined using the cell counting kit-8 (CCK-8) and a cell cycle assay was performed. Results: The apoptosis rate of PDL cells in PMSC-CM was significantly lower than that in HBSS at 24 hours (P < .001), while the two groups showed similar cell apoptosis rates at 6 and 12 hours (P > .05). The cell proliferation of PDLSCs treated with PMSC-CM significantly increased compared with the HBSS group (P < .05). The cell cycle assay revealed that the PDLSCs treated with HBSS were arrested at the G1 phase, while there was no difference between the PMSC-CM group and the control group (P > .05). Conclusions: Compared with HBSS, PMSC-CM showed better inhibition of apoptosis of PDL cells and promoted the proliferation of PDLSCs. Thus, PMSC-CM could be a promising storage medium for avulsed teeth. K E Y W O R D S cell viability, conditioned medium from placenta-derived mesenchymal stem cells, periodontal ligament cells, storage media, tooth avulsion How to cite this article: Ji L-L, Song G, Jiang L-M, et al. Evaluation of conditioned medium from placenta-derived mesenchymal stem cells as a storage medium for avulsed teeth:

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“…The expression levels of miRNAs have profound effects on cancer progression and human carcinogenesis (15). The expression of miR-296-5p has been shown to be significantly downregulated in lung cancer (3,16,17), breast cancer (18), diabetes (19)(20)(21) and other types of cancer and diseases (17,22,23). Therefore, exploring the function of miR-296-5p and the role of its possible target genes is essential to the understanding of the molecular mechanism of this miRNA in NSCLC.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-296, a suppressor non-coding RNA, downregulates SGLT2 expression in lung cancer

Zhang

Liu

et al. 2018

Int J Oncol

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Non-small cell lung cancer (NSCLC) is one of the most common types of cancer worldwide and has the highest mortality rate in China. MicroRNAs (miRNAs or miRs) are involved in tumorigenesis and their important role in cancer is becoming increasingly apparent. The expression of miR-296-5p in particular has been shown to be significantly downregulated in lung cancer. Sodium-glucose co-transporter-2 [SGLT2, also known as solute carrier family 5 member 2 (SLC5A2)] is an oncogene that promotes tumorigenesis. In this study, we aimed to determine the role of miR-296-5p in lung cancer and whether this involves the targeting of SGLT2. For this purpose, we examined miR-296-5p and SGLT2 expression in human lung cancer samples and cell lines by RT-qPCR and western blot analysis. In addition, the data analysis website TCGA was used for survival analysis with respect to SGLT2 expression. The effects of miR-296-5p were also examined on cell proliferation and cell cycle progression using respective assays. The results demonstrate that miR-296-5p is significantly downregulated in NSCLC tissues. Additionally, it is demonstrated that SGLT2 is directly targeted by miR-296-5p. Furthermore, our data reveal that the knockdown of SGLT2 using siRNA inhibits cell proliferation and impedes cell cycle progression. Collectively, data suggest that miR-296-5p not only inhibits NSCLC by downregulating SGLT2 expression, but also acts as a novel regulator of aberrant lung cancer cells to limit lung cancer progression.

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PDGF-BB Carried by Endothelial Cell–Derived Extracellular Vesicles Reduces Vascular Smooth Muscle Cell Apoptosis in Diabetes

Cited by 46 publications

References 55 publications

Perivascular adipose tissue–derived extracellular vesicle miR‐221‐3p mediates vascular remodeling

Perivascular adipose tissue–derived extracellular vesicle miR‐221‐3p mediates vascular remodeling

Evaluation of conditioned medium from placenta‐derived mesenchymal stem cells as a storage medium for avulsed teeth: An in vitro study

MicroRNA-296, a suppressor non-coding RNA, downregulates SGLT2 expression in lung cancer

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