PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

Abstract

This study aimed to develop a functionally graded membrane (FGM) to prevent infection and promote tissue regeneration. Poly( l -lactide-co- d , l -lactide) encapsulating platelet-derived growth factor (PDLLA-PDGF) or metronidazole (PDLLA-MTZ) was electrospun to form a nanofibrous layer on the inner or outer surface of a clinically available collagen membrane, respectively. The membrane was characterized for the morphology, molecule release profile, in vitro and in vivo biocompatibility, and preclinical efficiency for alveolar ridge regeneration. The PDLLA-MTZ and PDLLA-PDGF nanofibers were 800–900 nm in diameter, and the thicknesses of the functional layers were 20–30 μm, with sustained molecule release over 28 days. All of the membranes tested were compatible with cell survival in vitro and showed good tissue integration with minimal fibrous capsule formation or inflammation. Cell proliferation was especially prominent on the PDLLA-PDGF layer in vivo. On the alveolar ridge, all FGMs reduced wound dehiscence compared with the control collagen membrane, and the FGM with PDLLA-PDGF promoted osteogenesis significantly. In conclusion, the FGMs with PDLLA-PDGF and PDLLA-MTZ showed high biocompatibility and facilitated wound healing compared with conventional membrane, and the FGM with PDLLA-PDGF enhanced alveolar ridge regeneration in vivo. The design represents a beneficial modification, which may be easily adapted for future clinical use.

Advance of Nano-Composite Electrospun Fibers in Periodontal Regeneration

“…Bone morphogenetic proteins (BMPs) were widely reported to enhance bone reconstruction in GBR (Shalumon and Jyh-Ping, 2015). Ho et al (2017) incorporated platelet-derived growth factor (PDGF), a potent mitogen, into the nanofibers to facilitate alveolar ridge regeneration.…”

Advances of nanomaterial applications in oral and maxillofacial tissue regeneration and disease treatment

“…Other electrospun based materials include PLGA (Inanç, Arslan, Seker, Elçin, & Elçin, 2009), type I collagen/PCL/nHA (Col/PCL/nHA) (Wu et al, 2014), nHA/PCL (Yang, Both, Yang, Walboomers, & Jansen, 2009), nHA‐coated silk (Yang et al, 2013), and gelatin methacrylate (GelMA)/nHA microgel arrays (Zhao et al, 2016) nanofibrous scaffolds, which are associated with enhanced cells osteogenesis differentiation. Biomimetic nanofiber membrane composed with fish collagen/bioactive glass/chitosan (Col/BG/CTS), electrospun bi‐layered membranes (poly l ‐lactide‐co‐ d,l ‐lactide) encapsulating platelet‐derived growth factor (PDLLA‐PDGF) inner layer and encapsulating metronidazole (PDLLA‐MTZ) outer layer, or one layer was SF/PCL‐PEG‐PCL incorporating nano‐calcium phosphate (SPCA), another layer is PCL membrane), PDLLA/PLGA electrospun membranes coated with nHA, have been shown to enhanced bone formation (Bottino et al, 2012; Higuchi et al, 2019; Ho et al, 2017; Ma et al, 2018; Rakhmatia et al, 2013; Soltani Dehnavi et al, 2018; Türkkan et al, 2017; Zhou, Liu, et al, 2017).…”

Nanomaterials in Craniofacial Tissue Regeneration: A Review

“…Clinically available collagen membranes may be functionalized by electrospinning poly-DL-lactic acid (PDLLA) polymers to enhance periodontal regeneration. In fact, membranes functionalized with antibiotics and growth factor coated PDLLA nanofibers can facilitate healing and regenerative processes while reducing the risk of bacterial infection [27].…”