Peanut Allergenicity and Cross-Reactivity with Pea Proteins in the In Vivo Model

Abstract

The aim of the study was to analyse the potential pea-peanut cross-reactivity using the mice BALB/c as a biological in vivo model in the research on immune response to peanut proteins (PnE). BALB/c mice were three-fold sensitised (on days 1, 7, and 21) by oral or intraperitoneal (IP) administration of PnE in 0.5 mg or 1 mg dose, with or without adjuvant -aluminum hydroxide gel (Alum). Serum immunoglobulins (IgE, IgG, IgG1 and IgG2a) and level of cytokines (IL-4, IL-10, IFN-γ), secreted by the isolated lymphocytes were examined. The highest increase in total IgE and peanut-specific IgG1 was noted in the group sensitised by IP administration of PnE in the presence of Alum. Lymphocytes from peanut-sensitised (with and without Alum) mice showed a significantly high level of IL-4 and this cytokine was secreted to a much higher extent as compared to IFN-γ. Stimulation of a culture of lymphocytes with pea proteins resulted in high IFN-γ secretion. A weak reaction of peanut-specific IgG1 present in mice serum with pea globulins (vicilin -PV and legumin -PL) can suggest that the cross-reactivity between peanut and pea proteins results from the presence of proteins other than 7S and 11S globulins. Due to the demonstrated low cross-reactivity between peanut proteins and pea globulins, the possibility of applying pea proteins in peanut-allergy immunotherapy may be suggested.

Safety of mung bean protein as a novel food pursuant to Regulation (EU) 2015/2283

“…Data on clinical relevance and incidence of allergy to mung bean are scarce and mung‐bean allergic individuals are mainly identified in India, where the beans are commonly consumed (Misra et al., 2011 ). Furthermore, cross‐reactivity among legume proteins have been reported (Jensen et al., 2008 ; Szymkiewicz and Chudzik‐Kozłowska, 2013 ; Verma et al., 2013 ). Homologies between mung bean proteins and those of soybean, peanut and lupin, calculated by the applicant using the BLAST program in the database UniProtKB reference proteomes plus SwissProt were shown to be higher than 50%.…”

In Silico Analysis of Peanut ( Arachis Hypogaea L.) Allergenic Proteins, Mainly of Ara H 9, to Predict Epitopes in Cross-Reacting Plants

“…Dotychczasowe badania potwierdzają powstawanie reakcji krzyżowych między białkami orzecha ziemnego i innymi białkami roślin, zwłaszcza strączkowych (np. soi, soczewicy) oraz między epitopami białek orzechów różnych gatunków [1,20,22,25]. Największe prawdopodobieństwo wystąpienia reakcji krzyżowej istnieje między orzechem włoskim a laskowym oraz orzechem pekan [6].…”