Pectin co-functionalized dual layered solid lipid nanoparticle made by soluble curcumin for the targeted potential treatment of colorectal cancer

Mohamed, Jamal Moideen Muthu; Alqahtani, Ali; Ahmad, Fazil; Venkatesan, Krishnaraju; Kalpana, K.

doi:10.1016/j.carbpol.2020.117180

Cited by 85 publications

(46 citation statements)

References 71 publications

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“…Healthy eggs, embryos, and larvae were produced after 3 h of spawning. After 3 h of post fertilization (hpf), eggs were collected and ten healthy embryos were placed into a 24-well culture plate containing 1 mL of E3 media [ 53 ]. The control was maintained as a placebo and the samples (25–300 µg/mL) were treated for 96 h. The embryos were examined daily for their morphological changes, hatching, and survival rate, edema, spine dislocation, non-depleted yolk, tail bend as indicators of toxicity.…”

Section: Methodsmentioning

confidence: 99%

Preparation of Soluble Complex of Curcumin for the Potential Antagonistic Effects on Human Colorectal Adenocarcinoma Cells

Mohamed¹,

Alqahtani

Khan

et al. 2021

Pharmaceuticals

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This study was designed to investigate the effects of curcumin (CMN) soluble complex (SC) prepared by melt casting (HM) and hot-melt extrusion (HME) technology. Phase solubility (PS) study, in silico molecular modeling, aqueous solubility, drug release, and physicochemical investigation including a novel dyeing test was performed to obtain an optimized complex by a central composite design (CCD). The results show that the HME-SC produces better improvements towards solubility (0.852 ± 0.02), dissolution (91.87 ± 0.21 % at 30 min), with an ideal stability constant (309 and 377 M−1 at 25 and 37 °C, respectively) and exhibits AL type of isotherm indicating 1:1 stoichiometry. Intermolecular hydrogen bonding involves the formation of SC, which does not undergo any chemical modification, followed by the complete conversion of the amorphous form which was identified by XRD. The in vitro cytotoxicity showed that IC50 was achieved in the SW480 (72 µM.mL−1) and Caco-2 (40 µM.mL−1) cells while that of pure CMN ranged from 146 to 116 µM/mL−1. Apoptosis studies showed that cell death is primarily due to apoptosis, with a low rate of necrosis. In vivo toxicity, confirmed by the zebrafish model, exhibited the safety of the HME-SC. In conclusion, the HME-SC potentially enhances the solubility and cytotoxicity to the treatment of colorectal cancer (CRC).

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Section: Methodsmentioning

confidence: 99%

Preparation of Soluble Complex of Curcumin for the Potential Antagonistic Effects on Human Colorectal Adenocarcinoma Cells

Mohamed¹,

Alqahtani

Khan

et al. 2021

Pharmaceuticals

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“…The thermal characteristics of the scaffold were investigated using a differential scanning calorimeter (DSC) in order to measure their crystallisation temperature (TC) and melting temperature. The analysis was performed at a heating rate of 20 • C/min, from 0 to 1000 • C, under nitrogen gas with a flow rate of 10 mL/min [42]. For the determination of the thermostability of the scaffold, thermogravimetric analysis (TGA) was conducted under 50 mL/min nitrogen gas with a heating rate of 10 • C/min in the range from 0 to 1000 • C by a thermogravimetric curve.…”

Section: Thermal Propertiesmentioning

confidence: 99%

Human Hair Keratin Composite Scaffold: Characterisation and Biocompatibility Study on NIH 3T3 Fibroblast Cells

et al. 2021

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The aim of this study was to transform human hair keratin waste into a scaffold for soft tissue engineering to heal wounds. The keratin was extracted using the Shindai method. Keratin and polyvinyl alcohol (PVA) was cross-linked with alginate dialdehyde and converted into a scaffold by the freeze-drying method using gentamycin sulphate (GS) as a model drug. The scaffold was subjected to Fourier transform infrared spectra (FTIR), swelling index, porosity, water absorption, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), drug release, and cell viability (MTT) analysis. The scaffold was tested for keratinocyte growth using the murine fibroblast cell line (NIH 3T3 cells). The outcome from the keratin had a molecular weight band between 52–38 kDa in SDS-PAGE (Sodium dodecylsulfate-Polyacrylamide gel electrophoresis). A porous scaffold was capable of water absorption (73.64 ± 14.29%), swelling ability (68.93 ± 1.33%), and the release of GS shown as 97.45 ± 4.57 and 93.86 ± 5.22 of 1:4 and 1:3 scaffolds at 16 h. The physicochemical evaluation revealed that the prepared scaffold exhibits the proper structural integrity: partially crystalline with a strong thermal property. The scaffold demonstrated better cell viability against the murine fibroblast cell line (NIH 3T3 cells). In conclusion, we found that the prepared composite scaffold (1:4) can be used for wound healing applications.

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“…The cytotoxicity of the samples against the SW480 and Caco-2 cell lines were estimated utilizing MTT assay [13]. Briefly, the cells were seeded into 96-well culture plates at a cell density of 5×10 3 cells/mL (200 µL/well).…”

Section: In Vitro Cytotoxicity Studymentioning

confidence: 99%

Studies on Preparation and Evaluation of Soluble 1:1 Stoichiometric Curcumin Complex for Colorectal Cancer Treatment

Mohamed¹,

Ahmad

Alqahtani

et al. 2021

Trends Sci

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This study investigates the complex of curcumin (CMN), which has enhanced solubility and hence, higher cytotoxicity compared to free CMN. Insilco molecular modelling and phase solubility (PS) studies were performed with the drug and carriers for interaction. The complex was characterized by in vitro drug release, FT-IR, PXRD, TGA, DSC, SEM, DLS, and functionalized dyeing test. The result showed that the CMN-PEG6000 complex produced significant properties of solubility (≈ 190 folds) and dissolution (80.68 % at 30 min), with stability constants equivalent to 309 and 377 M-1 at 25 and 37 °C, respectively. It exhibited AL type of isotherm indicating 1:1 stoichiometry. The result from the in vitro cytotoxicity showed that 50 % inhibition (IC50) was achieved on the SW480 and Caco-2 cells at an amount of complex that was considerably lesser than free CMN. Apoptosis study showed that the cells underwent cell death mainly by apoptosis with a small number by necrosis. HIGHLIGHTS Enhanced curcumin solubility up to 190 fold higher than pure curcumin was investigated The phase solubility results of curcumin range from 5.7×10-4 M-1 and 7.8×10-4 M-1 at 25 and 37 °C, respectively First time of novel dyeing test was performed with complex of curcumin, signified its solubility This study provides useful approach for obtaining curcumin products with maximum aqueous solubility GRAPHICAL ABSTRACT

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Pectin co-functionalized dual layered solid lipid nanoparticle made by soluble curcumin for the targeted potential treatment of colorectal cancer

Cited by 85 publications

References 71 publications

Preparation of Soluble Complex of Curcumin for the Potential Antagonistic Effects on Human Colorectal Adenocarcinoma Cells

Preparation of Soluble Complex of Curcumin for the Potential Antagonistic Effects on Human Colorectal Adenocarcinoma Cells

Human Hair Keratin Composite Scaffold: Characterisation and Biocompatibility Study on NIH 3T3 Fibroblast Cells

Studies on Preparation and Evaluation of Soluble 1:1 Stoichiometric Curcumin Complex for Colorectal Cancer Treatment

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