Pectin coating improves physicochemical properties of caseinate/zein nanoparticles as oral delivery vehicles for curcumin

Chang, Chao; Wang, Taoran; Hu, Qiaobin; Zhou, Mingyong; Xue, Jingyi; Luo, Yangchao

doi:10.1016/j.foodhyd.2017.03.033

Cited by 242 publications

(104 citation statements)

References 42 publications

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“…Coating protein-based complex nanoparticles with polysaccharide is commonly studied to improve the GI-stability for oral delivery applications. During the past few years, our lab has developed a series of polysaccharide-coated protein complex nanoparticles [10,14,15]. We found that the type of polysaccharide and the use of chemical crosslinkers are the two major factors determining the GI-stability improvement.…”

Section: Introductionmentioning

confidence: 99%

Oxidized Dextran as a Macromolecular Crosslinker Stabilizes the Zein/Caseinate Nanocomplex for the Potential Oral Delivery of Curcumin

Rodriguez

Luo

2019

Molecules

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In this study, we prepared complex nanoparticles from a combination of two proteins and one polysaccharide for the encapsulation and delivery of lipophilic bioactive compounds. Two proteins, zein and sodium caseinate (NaCas), provided a hydrophobic core for the encapsulation of a lipophilic compound (curcumin), while a polysaccharide dialdehyde, oxidized dextran, served as the coating material and macromolecular crosslinker to create covalent linkage with two proteins for stabilization purposes. The heating time and crosslinker concentration were optimized to achieve the desirable colloidal stability in simulated gastric and intestinal fluids. Our results suggested that heating time played a more important role than the concentration of oxidized dextran. The optimized complex nanoparticles had a particle size of around 150 nm with a PDI < 0.1 and negative surface charge. Morphological observation by transmission electron microscopy revealed a spherical shape and uniform size distribution. Fourier transform infrared and fluorescence spectroscopies evidenced the formation of Schiff base complex, confirming the validity of covalent crosslinking. Furthermore, the complex nanoparticles demonstrated superior encapsulation properties for curcumin, showing an efficiency of >90% at 10% loading. A rather slow kinetic release profile of curcumin from complex nanoparticles was observed under simulated gastrointestinal conditions. The complex nanoparticles prepared from zein, NaCas, and oxidized dextran hold promising potential for the oral delivery of lipophilic bioactive compounds.

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Section: Introductionmentioning

confidence: 99%

Oxidized Dextran as a Macromolecular Crosslinker Stabilizes the Zein/Caseinate Nanocomplex for the Potential Oral Delivery of Curcumin

Rodriguez

Luo

2019

Molecules

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“…Recently, the protein and polysaccharide complexes have been used as the coating layer for zein particles and the formed particles have shown an improved physical stability in acidic aqueous environment by having a thicker coating layer and stronger steric repulsion [13]. Some previous studies have shown that the coating of caseinate-pectin complex particles could enhance the stability of zein particles under simulated gastrointestinal conditions and meanwhile improve their encapsulation capacity [14,15]. Nevertheless, the fabrication for zein-caseinate-pectin complex particles was achieved via a pH- and heating-induced electrostatic adsorption process.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Zein Composite Particles Coated by Caseinate-Pectin Electrostatic Complexes with Improved Structural Stability in Acidic Aqueous Environments

Zhang

Wang

et al. 2019

Molecules

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Zein composite particles coated with caseinate-pectin electrostatic complexes (zein-caseinate-pectin particles) were fabricated using an electrostatic deposition and liquid-liquid dispersion method without heating treatment. Compared to zein particles coated only with caseinate, the acidic stability of zein-caseinate-pectin particles was greatly improved, and the particle aggregation was suppressed at pH 3–6, especially at pH values near the isoelectric point of caseinate (pH 4–5). Besides, desirable long-term storage stability and re-dispersibility were observed. Under different zein to curcumin (Cur) feeding ratios (10:1, 20:1, 30:1 and 40:1, w/w), the Cur-loaded zein-caseinate-pectin particles had a spherical shape with an average diameter ranging from 358.37 to 369.20 nm, a narrow size distribution (polydispersity index < 0.2) and a negative surface charge ranging from −18.87 to −19.53 mV. The relatively high encapsulation efficiencies of Cur (81.27% to 94.00%) and desirable re-dispersibility were also achieved. Fluorescence spectroscopy indicated that the encapsulated Cur interacted with carrier materials mainly through hydrophobic interactions. The in-vitro release profile showed a sustained release of Cur from zein-caseinate-pectin particles in acidic aqueous environment (pH 4) up to 24 h, without any burst effect. In addition, the encapsulation retained more ABTS•+ radical scavenging capacity of Cur during 4 weeks of storage. These results suggest that zein-caseinate-pectin particles may be used as a potential delivery system for lipophilic nutrients in acidic beverages.

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“…The in vitro release assay was performed according to Chang et al with some modifications. The nanoparticle suspension (2 mL) containing the botanicals was placed in dialysis membrane bags (1 kDa exclusion pore, SpectraPore Waltham, USA) and immersed in 100 mL solution of 3% Pluronic F68 (w/v).…”

Section: Methodsmentioning

confidence: 99%

Association of zein nanoparticles with botanical compounds for effective pest control systems

Oliveira

Campos

Germano-Costa

et al. 2019

Pest Management Science

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BACKGROUND: Botanical compounds from plant species are known to have pesticidal activity and have been used in integrated pest management programs. The varied spectrum of the pesticidal action of these compounds can also avoid selection of resistance in pest populations. In this study, mixtures of the botanical compounds geraniol, eugenol and cinnamaldehyde were encapsulated in zein nanoparticles to improve their stability and efficiency. Biological effects of the nano-scale formulations of the botanical compounds were evaluated against two agricultural pests: the two-spotted spider mite (Tetranychus urticae) and the soybean looper (Chrysodeixis includes).RESULTS: The formulations were stable over time (120 days) with a high encapsulation efficiency (>90%). Nanoencapsulation also provided protection against degradation of the compounds during storage and led to a decrease in toxicity to non-target organisms. The release of the compounds (especially eugenol and cinnamaldehyde) from the nanoparticles was directly influenced by temperature, and the main mechanism of release was through a diffusion-based process. Nanoencapsulated compounds also showed superior efficiency compared to the emulsified compounds in terms of repellency and insecticidal activity. CONCLUSION:The findings of this study indicate that the convergence of botanical compounds with nano-scale formulation has the potential to improve efficacy for their sustainable use in integrated pest management in agriculture.

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Pectin coating improves physicochemical properties of caseinate/zein nanoparticles as oral delivery vehicles for curcumin

Cited by 242 publications

References 42 publications

Oxidized Dextran as a Macromolecular Crosslinker Stabilizes the Zein/Caseinate Nanocomplex for the Potential Oral Delivery of Curcumin

Oxidized Dextran as a Macromolecular Crosslinker Stabilizes the Zein/Caseinate Nanocomplex for the Potential Oral Delivery of Curcumin

Fabrication and Characterization of Zein Composite Particles Coated by Caseinate-Pectin Electrostatic Complexes with Improved Structural Stability in Acidic Aqueous Environments

Association of zein nanoparticles with botanical compounds for effective pest control systems

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