Pectin hydrogels crosslinked via peptide nanofibers for designing cell-instructive dynamic microenvironment

Uslu, Cemile; Narin, Sümeyye; Demirsoy, Zeynep; Öksüz, Hasan Basri; Gülseren, Gülcihan

doi:10.1016/j.ijbiomac.2023.123604

Cited by 5 publications

(5 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibacterial activity of three experimental groups, pectin, PP, and HT‐PP was assessed with a new method that mimics the 3D environment of the dental tissue. In this method, coatings were prepared inside molds that swim in a medium [ 32 ] (Figure S6, Supporting Information), enabling a dynamic environment for the bacterial cells together with the rotational motion throughout the incubation. This dynamic environment simulates the changing mechanical pressures applied to dental tissue.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Bioinspired Multi‐Layer Biopolymer‐Based Dental Implant Coating for Enhanced Osseointegration

Üzülmez

Demirsoy

Can

et al. 2023

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

The major drawbacks of metal‐based implants are weak osseointegration and post‐operational infections. These limitations restrict the long‐term use of implants that may cause severe tissue damage and replacement of the implant. Recent strategies to enhance the osseointegration process require an elaborate fabrication process and suffer from post‐operative complications. To address the current challenges taking inspiration from the extracellular matrix (ECM), the current study is designed to establish enhanced osseointegration with lowered risk of infection. Natural biopolymer pectin, peptide amphiphiles, and enzyme‐mimicking fullerene moieties are governed to present an ECM‐like environment around the implant surfaces. This multifunctional approach promotes osseointegration via inducing biomineralization and osteoblast differentiation. Application of the biopolymer‐based composite to the metal surfaces significantly enhances cellular attachment, supports the mineral deposition, and upregulates osteoblast‐specific gene expression. In addition to the osteoinductive properties of the constructed layers, the inherent antimicrobial properties of multilayer coating are also used to prevent infection possibility. The reported biopolymer‐artificial enzyme composite demonstrates antimicrobial activity against Escherichia coli and Bacillus subtilis as a multifunctional surface coating.

show abstract

Section: Resultsmentioning

confidence: 99%

“…It has also been previously shown that pectin and peptide support osteogenic differentiation. [ 32 ] The synergistic effect of each component of dental coating resulted in notable gene expression levels via enzymatic and structural promotion.…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Multi‐Layer Biopolymer‐Based Dental Implant Coating for Enhanced Osseointegration

Üzülmez

Demirsoy

Can

et al. 2023

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cells were incubated under standard cell culture conditions: 37 °C, 5% CO 2 , and 20% O 2 . Adherent HUVECs were harvested by trypsin-EDTA and collected by centrifugation at 3500 rpm for 5 min, and the cell pellet was dissolved in the required amount of medium for further experiments …”

Section: Materials and Methodsmentioning

confidence: 99%

Ultrasmall Gold Nanospheres Engineered with Nisin Pectin Bioconjugate: A Multifunctional Solution for Dye Removal, Detoxification, and Antimicrobial Applications

Demirsoy,

Sara,

Aysan

et al. 2023

ACS Appl. Eng. Mater.

Self Cite

View full text Add to dashboard Cite

Contamination of water sources with effluents like toxic dye molecules, organophosphorus (OP) compounds, and pathogenic bacteria is a major public concern, since those contaminants cause serious health problems in humans, animals, and plants. Thus, there is an urgent need for solutions to clean water sources from toxic and harmful pollutants. Gold nanoparticles exhibit remarkable potential for the remediation of contaminated water sources due to their essential catalytic and antimicrobial attributes. Nevertheless, the existing synthesized gold nanoparticles have not yet achieved the desired triple-action capability of simultaneously eliminating toxic dye molecules, organophosphorus (OP) compounds, and pathogenic bacteria. To address this challenge, we designed ultrasmall gold nanospheres that incorporate a bioconjugate of nisin antimicrobial peptide and pectin (NisPec). The resulting nanoparticles, termed AuNP@NisPec, demonstrated the ability to generate reactive oxygen species (ROS), facilitating the decolorization and detoxification of harmful dye molecules. The synergistic relationship between the gold nanospheres and the NisPec polymer allowed these nanoparticles to hydrolyze the phosphoester bond, rendering them a potential solution for OP toxicity. Additionally, the NisPec-decorated gold nanospheres exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. This innovative approach offers a comprehensive and holistic solution to address water contamination by simultaneously tackling multiple challenges.

show abstract

“…Transwell Penetration Assay. 100.000 SHSY-5Y cells were seeded to the wells of a 12-well plate, and 300.000 SHSY-5Ycells were encapsulated in a 2% pectin-10 mM peptide gel 26 on a transwell insert. For the control group, the transwell insert remained empty.…”

Section: Ros Assaymentioning

confidence: 99%

“…To explore the brain tissue penetration profile of bimetallic NPs, we established a transwell penetration model. On the upper side of the transwell, a 3D tissue model with a high concentration of cells (3 × 10 5 ) encapsulated in hydrogels 26 was generated. Cells were also seeded at the bottom of the well to assess the transfer and penetration success of the NPs.…”

Section: Mechanistic Investigation Of Au/ag-npsmentioning

confidence: 99%

Exploring Bimetallic Nanoparticles in Alzheimer’s Therapy: A Novel Bio-Assisted Synthesis with Multitarget Potential

Gülseren,

Demirsoy,

Şeker

et al. 2024

Mol. Pharmaceutics

Self Cite

View full text Add to dashboard Cite

Unlocking the potential of metal nanoparticles (NPs) in biomedical applications represents a leading endeavor in contemporary research. Among these, gold NPs (AuNPs) and silver NPs (AgNPs) have shown promising strides in combatting complex neurodegenerative ailments like Alzheimer's disease. Yet, the unexplored realm of bimetallic Au/Ag-NP harbors immense potential, concealing undiscovered opportunities for enhanced therapeutic effectiveness through the synergistic interaction of metal ions. Nonetheless, the limitations of traditional synthesis methods have restricted the preparation, biocompatibility, and versatility of these NPs, prompting an urgent requirement for innovative approaches. Biobased synthetic methodologies have emerged as a noteworthy solution to address these challenges. Our study ventures into uncharted terrain, harnessing collagen-mimicking peptide nanofibers as a bioactive template for the synthesis of bimetallic NPs. These green NPs exhibit remarkable activity in inhibiting amyloid β (Aβ) protein aggregation with almost 74% inhibition, surpassing the individual impacts of Au and Ag NPs, which show inhibition percentages of 66 and 43, respectively. The bimetallic Au/Ag-NPs not only demonstrate powerful inhibition of Aβ, but they also demonstrate inhibitory activity against esterase (∼50%) and against reactive oxygen species (ROS) (∼75%), metamorphosing into multifaceted therapeutic agents for Alzheimer's disease. Au/Ag-NPs have proven highly beneficial in surpassing cellular barriers, as evidenced by studies on tissue penetration, 3D uptake, and endosomal escape, and these attributes also hold promise for the future treatment modalities. The findings indicate that the intrinsic traits of Au/Ag-NPs provide numerous mechanistic benefits, such as inhibiting Aβ and acetylcholinesterase (AChE), and reducing stress related to ROS, in addition to their advantageous internalization properties. This research represents a notable advancement in the development of multitargeted treatments for neurodegenerative disorders using bimetallic NPs, diverging from the prevalent emphasis on AuNPs in the current literature.

show abstract

Pectin hydrogels crosslinked via peptide nanofibers for designing cell-instructive dynamic microenvironment

Cited by 5 publications

References 32 publications

Bioinspired Multi‐Layer Biopolymer‐Based Dental Implant Coating for Enhanced Osseointegration

Bioinspired Multi‐Layer Biopolymer‐Based Dental Implant Coating for Enhanced Osseointegration

Ultrasmall Gold Nanospheres Engineered with Nisin Pectin Bioconjugate: A Multifunctional Solution for Dye Removal, Detoxification, and Antimicrobial Applications

Exploring Bimetallic Nanoparticles in Alzheimer’s Therapy: A Novel Bio-Assisted Synthesis with Multitarget Potential

Contact Info

Product

Resources

About