Pectinolytic, Indole-Positive Strains of Klebsiella pneumoniae

Riesen, V. Lyle Von

doi:10.1099/00207713-26-2-143

Cited by 26 publications

(16 citation statements)

References 11 publications

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“…In the present survey, 44.50C was employed to be consistent with U.S. standards for the operational definition of an FC (1). The discovery that these FC-isolates grow at 100C and that indole production correlates with pectin liquefaction adds significance to the previously proposed species status of K. oxytoca (20,21). Group II isolates resemble K. pneumoniae isolates of group I but are distinguished from them by reciprocal responses in growth at 100C and a negative response in the fecal coliform test.…”

Section: Discussionmentioning

confidence: 64%

“…Production of indole from tryptophan was determined by standard techniques (9). Pectin liquefaction was detected on a plate medium (9,21) in disposable petri dishes (15 by 100 mm). The test culture was heavily inoculated in a localized area such that after growth the colony was some 10 mm in diameter.…”

Section: Methodsmentioning

confidence: 99%

“…By using the technique of deoxyribonucleic acid hybridization, considerable molecular heterogeneity was demonstrated among isolates of Klebsiella and in particular those derived from the environment (16). Others have commented on published inconsistencies in the methyl red and Voges-Proskauer reactions, whereas some have recommended species status (K. oxytoca) for indole-producing (I+), anaerogenic Klebsiella isolates (5,20,21). Von Reisen demonstrated that 11 of 11 I+ Klebsiella also hydrolyze pectin, but no documented studies were made on indole-negative (I-) isolates (21).…”

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confidence: 99%

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Significance of low-temperature growth associated with the fecal coliform response, indole production, and pectin liquefaction in Klebsiella

Naemura¹,

Seidler²

1978

Appl Environ Microbiol

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In the genus Klebsiella, the growth response in nutrient broth at 100C correlates inversely with the operational definition of a fecal coliform and not merely with the ability to grow at 44.5°C. Of the fecal coliform-positive Klebsiella, 97% did not grow at 100C after 72 h of incubation. Conversely, 97% of the fecal coliformnegative isolates grew at 10°C. The amount of growth at 100C varied among the fecal coliform-negative isolates and was found to correlate with indole production and pectin liquefaction. Low-temperature growth associated with specific biochemical tests can be used to differentiate several groups in the genus Klebsiella. Three main groups were discerned. Group I consists of indole-negative, pectinnonliquefying, fecal coliform-positive isolates that do not grow at 100C. Group II isolates are differentiated from group I by a fecal-coliform-negative response and growth at 10°C. Group III are indole-positive, pectin-liquefying, fecal coliformnegative isolates that grow at 100C. In our culture collection, isolates of group I are most frequently of human/animal clinical origins, whereas isolates of groups II and III are predominantly derived from the environment.

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Section: Discussionmentioning

confidence: 64%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Significance of low-temperature growth associated with the fecal coliform response, indole production, and pectin liquefaction in Klebsiella

Naemura¹,

Seidler²

1978

Appl Environ Microbiol

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“…Indole production in klebsiellas has been correlated with the liquefaction of gelatin (Lautrop, 1956;Stenzel, Burger and Mannheim, 1972) and pectin (von Riesen, 1976), and such strains have been termed Klebsiella oxytoca. We found a distinct group of klebsiella strains that were FC negative and produced indole and liquefied pectin.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Properties of Klebsiella Strains Isolated From Different Sources

Edmondson

Cooke

Wilcock

et al. 1980

Journal of Medical Microbiology

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“…The indole positive Klebsiella strains are considered to belong to a separate species, oiz., K1. oxytoca (Stenzel et al 1972;Jain et al 1974;von Riesen 1976;Skerman et al 1980). The protein patterns of 'H.…”

Section: Strains Identijed By the Api 20e Systemmentioning

confidence: 99%

The Identification of Enterobacteriaceae from Breweries: Combined Use and Comparison of API 20E System, Gel Electrophoresis of Proteins and Gas Chromatography of Volatile Metabolites

Vuuren

Kersters

Ley

et al. 1981

Journal of Applied Bacteriology

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The API 20E system was applied and evaluated for the identification of 60 bacterial contaminants, isolated from South African lager beer breweries and tentatively identified as Enterobacteriaceae. The results were compared with the groupings obtained by polyacrylamide gel electrophoresis of soluble proteins and by gas chromatography of volatile metabolites. Numerical analysis of the results obtained by all three methods revealed an excellent overall agreement. The API 20E system proved to be a rapid and fairly accurate (90%) method for identification of enterobacterial contaminants in beer breweries. Without referral to the API computer service, the API 20E system correctly identified 78 and 85% of the isolates to the species and genus level, respectively. Reference to the computer centre allowed an additional 5% of strains to be identified correctly to the species level.By combining the results of the three methods, 92% of the strains were finally identified as (numbers of strains in brackets): Enterohacter agglomeruns (I@, Ent. cloacae (9, Enterobucter sp. (4), 'Hufnia protea' (7), Hafnia alvei (3), Klebsiella pneumoniae (3), KI. oxytoca (7), Serratiu marcexens (9, Citrobacter freundii ( 2 ) and Proteus mirabilis (1). Five isolates remained unidentified.ENTEROBACTERIACEAE have been detected as contaminants in beer brewery samples, most commonly in prefermentation wort and in the early stages of fermentations ). In the present study 60 bacterial strains, isolated in South Africa as contaminants during the beer brewing process and tentatively identified as Enterobacteriaceae, were characterized by phenotypic tests using the API 20E system, by gas chromatographic analysis of volatile metabolites produced by the bacterial strains in a standardized growth medium, and by comparison of their respective electrophoretic protein patterns determined by gel electrophoresis. The latter technique enables the detection of strains with high genetic similarity (Kersters & De Ley 1975). Computerized numerical analysis was used to compare the results obtained by these three methods. It was possible to identify the majority (92%) of the isolates by the combined results of the three techniques. The taxonomic position of some strains was verified by deoxyribonucleic acid (DNA): ribosomal ribonucleic acid (rRNA) hybridizations.

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Pectinolytic, Indole-Positive Strains of Klebsiella pneumoniae

Cited by 26 publications

References 11 publications

Significance of low-temperature growth associated with the fecal coliform response, indole production, and pectin liquefaction in Klebsiella

Significance of low-temperature growth associated with the fecal coliform response, indole production, and pectin liquefaction in Klebsiella

A Comparison of the Properties of Klebsiella Strains Isolated From Different Sources

The Identification of Enterobacteriaceae from Breweries: Combined Use and Comparison of API 20E System, Gel Electrophoresis of Proteins and Gas Chromatography of Volatile Metabolites

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