Pectolitic Bacteria Activity of Genus Bacillus

Авдєєва, Л. В.; Осадча, А. I.; Safronova, L. A.; Iliash, V.M.; Хархота, М. А.

doi:10.18524/2307-4663.2010.3(11).99033

Cited by 3 publications

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“…Ëèòè÷åñêóю è öåëëюëîзîëèòè÷åñêóю àêòèâíîñòè îпðåäåëÿëè èзâåñò-íыìè ìåòîäàìè [1,6,9]. Ëèòè÷åñêóю àêòèâíîñòü âыðàжàëè â пðîöåíòàх ñíèжåíèÿ îпòè÷åñêîé пëîòíîñòè (%ΔÎП), ÷òî îòðàжàåò пðîöåíò ëèзèñà êëåòîê òåñò-êóëüòóðы.…”

Section: ìатериалы и методыunclassified

“…Ðàíåå íàìè пîêàзàíî, ÷òî èзó÷àåìыå пðîбèîòè÷åñêèå шòàììы B. subtilis ñпîñîбíы òàêжå ñèíòåзèðîâàòü è эêñêðåòèðîâàòü â êóëüòóðàëü-íóю жèäêîñòü êîìпëåêñ öåëëюëàз (Ñ х -, Ñ 1 -, Ñ 2 -фåðìåíòы è öåëëîбèàзы) [1], ÷òî ñòàëî îñíîâàíèåì пðåäпîëîжèòü ó÷àñòèå эêзîöåëëюëàз â àíòà-гîíèñòè÷åñêîé àêòèâíîñòè бàöèëë.…”

Section: ðезультаты и их обсуждениеunclassified

“…Äëÿ èзó÷åíèÿ ëèòè÷åñêîé è àíòèìèêðîбíîé àêòèâíîñòåé бàêòåðèè âыðàщèâàëè пðè 37 °Ñ â òå÷åíèå 24 ÷ â óñëîâèÿх гëóбèííîé êóëüòóðы íà êà÷àëêå пðè 200 îб/ìèí íà гëюêîзî-ìèíåðàëüíîé ñèíòåòè÷åñêîé ñðåäå ñëåäóющåгî ñîñòàâà (г/ë): гëюêîзà -10,0-15,0; íàòðèÿ öèòðàò -1,29; (NH 4 ) 2 HPO 4 -4,75, KH 2 PO 4 -9,6, MgSO 4 ⋅ 7H 2 O -0,18, ðÍ 7,0±0,2. Äëÿ èзó÷åíèÿ öåëëюëîзîëèòè÷åñêîé àêòèâíîñòè бàêòåðèè âыðàщèâàëè â òåх жå óñëîâèÿх íà ñðåäå ñ 0,5% öåëëюëîзы бåз гëюêîзы [1]. Â ðÿäå îпыòîâ â êà÷åñòâå äîпîëíèòåëüíîгî èñòî÷íèêà óгëåðîäíîгî пèòàíèÿ èñпîëüзîâàëè ëàêòèò èëè ëàêòóëîзó (пî 15%).…”

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Lytic Activity and Its Relationship With Celluloselytic and Antagonist Activity of Bacilli

Авдєєва¹,

Осадча²,

Хархота³

2011

Microbiology&Biotechnology

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Section: ìатериалы и методыunclassified

Section: ðезультаты и их обсуждениеunclassified

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Lytic Activity and Its Relationship With Celluloselytic and Antagonist Activity of Bacilli

Авдєєва¹,

Осадча²,

Хархота³

2011

Microbiology&Biotechnology

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“…Бèîñèíòåз öåëëюëàз ðàзëè÷íыìè шòàììàìè бàöèëë íàèбîëåå èíòåíñèâíî пðîòåêàåò пðè âåëè÷èíå ðН ñðåäы îò 6,0 äî 8,0 [1]. ðНîпòèìóì äåéñòâèÿ фåðìåíòîâ îбы÷íî òàêжå ëåжèò â эòèх пðåäåëàх зíà÷åíèé ðН, îäíàêî пî äàííыì ðÿäà èññëåäîâàòåëåé эòîò пîêàзàòåëü ìîжåò òàêжå íàхîäèòüñÿ è ìåжäó зíà÷åíèÿìè ðН 4,5-6,5 è èзìåíÿòüñÿ â зàâèñèìîñòè îò ñóбñòðàòà è äðóгèх óñëîâèé [6,10].…”

Section: результаты и их обсуждениеunclassified

“…С пîìîщüю îпòèìèзàöèè óñëîâèé êóëüòèâèðîâàíèÿ, ñîñòàâà ñðåäы è åå ðН äëÿ èññëåäóåìых бàêòåðèé íàì óäàëîñü óñèëèòü ó бàöèëë àêòèâíîñòü ðÿäà гèäðîëèòè÷åñêèх фåðìåíòîâ. Иññëåäóåìыå гèäðîëàзы пðîÿâëÿëè èíäóöèбåëüíыé òèп ñåêðåöèè, пîñêîëüêó èх пðîäóöèðîâàíèå íàбëюäàëîñü ëèшü â пðèñóòñòâèè â ñðåäå ñпåöèфè÷åñêèх ñóбñòðàòîâ [1,2]. Л.Â.…”

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Properties of the Cellulases Produced by Bacteria of Genus Bacillus

Авдєєва¹,

Осадча²,

Хархота³

2011

Microbiology&Biotechnology

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Изучаëи энзиматические свойства разëичныõ типов цеëëюëаз, проду� цируемыõ бациëëами при ãëубинном куëьтивировании на среде с тя� жеëоразрушаемой цеëëюëозой. Обобщены резуëьтаты иссëедований свойств цеëëюëаз шести наибоëее активныõ штаммов бациëë. Дан анаëиз вëияния рН и температуры на иõ активность и стабиëьность. Установëено, что цеëëюëазы изучаемыõ штаммов бациëë проявëяют наибоëьшую активность при рН от 6,0 до 7,0. Искëючение составëяет С 2-фермент, имеющий рН-оптимум в боëее кисëой зоне-от 5,0 до 8,0. Температурный оптимум действия С õ-, С 1-и С 2-ферментов-50 °С, цеëëобиазы-40 °С. Цеëëюëазы бациëë отëичаëись высокой термо� стабиëьностью, которую проявëяëи в широком интерваëе темпера� тур-от 40 °С до 70 °С. К ë ю ч е в ы е с ë о в а : цеëëюëазы, свойства, Bacillus.

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Formation and Functioning of Chaetomium Cochliodes / Fagopyrum Еsculentum Endophytic Association

Kopilov¹

2020

J microb biotech food sci

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Many representatives of soil saprotroph fungi can penetrate into plant tissues forming endophytic associations with a positive effect on both micro- and macroorganisms. The endophytic fungi may positive act on growth processes, immune status, and resistance of plants to stress factors. For some crops, including buckwheat, the ability to form endophytic associations with soil fungi remains unexplored. Thus the main objectives of our study were to establish the features of interaction between Chaetomium cochliodes3250 and buckwheat plants. Conventional biochemical (content of plant growth regulators, activity of succinate dehydrogenase, acid and alkaline phosphatase, exoglucanase, endoglucanase, β-glucosidase, polygalacturonase) and physicochemical (content of photosynthetic pigments) study methods were used during the work. We established the capability of C. cochliodes 3250 for growth-regulating substances synthesis. The fungus could produce indolyl 3-acetic acid, gibberellic acid, that stimulates growth and development of plants both with mediator molecules, synthesizes 2,4-epibrassinolide, cholesterol and ergosterol playing an important role in plant resistance against pathogens. We found out that C. cochliodes 3250 was capable for active synthesis of cellulase complex enzymes (exoglucanase, endoglucanase, β-glucosidase) and polygalacturonase, necessary for fungus penetration into the root tissues. Pre-sowing treatment of buckwheat seeds by fungus caused the main physiological responses of plants: an increase of total adsorption and active working surface of the roots, the length and weight of plants, the leaf area and the content of chlorophylls a and b. Thus, the ability of C. cochliodes3250 to form an efficient endophytic association with buckwheat plants been improved.

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Pectolitic Bacteria Activity of Genus Bacillus

Cited by 3 publications

References 0 publications

Lytic Activity and Its Relationship With Celluloselytic and Antagonist Activity of Bacilli

Lytic Activity and Its Relationship With Celluloselytic and Antagonist Activity of Bacilli

Properties of the Cellulases Produced by Bacteria of Genus Bacillus

Formation and Functioning of Chaetomium Cochliodes / Fagopyrum Еsculentum Endophytic Association

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