Peculiarities of accumulation of glucofructans in Allium L. (Amaryllidaceae) species

Vasfilova, Evgeniya; Vorobeva, Tatyana

doi:10.17223/19988591/42/8

Cited by 1 publication

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“…Stress-induced activity of transposable elements can potentially affect the expression of neighboring genes. The revealed polymorphism using iPBS amplification DNA profiling for both individuals and a population within a species may indicate relatively recent events, i.e., the activity of transposable elements [ 72 ]. Under the stressful conditions of the Altai Mountains, which are characterized by significant daily temperature drops, high radiation levels, and summer snowfalls, we observed differences between populations of endemic A. ledebourianum , which manifested themselves both in the morphometric parameters of plants and on the genome level.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, human economic activity is the main limiting factor of this type [ 11 , 73 ]. According to biodiversity studies of A. ledebourianum , more precisely about the study of the biological characteristics of the species during its introduction, or the results of biochemical studies are presented in the following works [ 51 , 72 , 74 ]. Studies of genetic polymorphism using genome in situ hybridization (GISH) and phylogenetic analysis have been carried out on this species of Allium [ 75 , 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

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Primer Binding Site (PBS) Profiling of Genetic Diversity of Natural Populations of Endemic Species Allium ledebourianum Schult.

Хапилина

Turzhanova

Danilova³

et al. 2021

BioTech

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Endemic species are especially vulnerable to biodiversity loss caused by isolation or habitat specificity, small population size, and anthropogenic factors. Endemic species biodiversity analysis has a critically important global value for the development of conservation strategies. The rare onion Allium ledebourianum is a narrow-lined endemic species, with natural populations located in the extreme climatic conditions of the Kazakh Altai. A. ledebourianum populations are decreasing everywhere due to anthropogenic impact, and therefore, this species requires preservation and protection. Conservation of this rare species is associated with monitoring studies to investigate the genetic diversity of natural populations. Fundamental components of eukaryote genome include multiple classes of interspersed repeats. Various PCR-based DNA fingerprinting methods are used to detect chromosomal changes related to recombination processes of these interspersed elements. These methods are based on interspersed repeat sequences and are an effective approach for assessing the biological diversity of plants and their variability. We applied DNA profiling approaches based on conservative sequences of interspersed repeats to assess the genetic diversity of natural A. ledebourianum populations located in the territory of Kazakhstan Altai. The analysis of natural A. ledebourianum populations, carried out using the DNA profiling approach, allowed the effective differentiation of the populations and assessment of their genetic diversity. We used conservative sequences of tRNA primer binding sites (PBS) of the long-terminal repeat (LTR) retrotransposons as PCR primers. Amplification using the three most effective PBS primers generated 628 PCR amplicons, with an average of 209 amplicons. The average polymorphism level varied from 34% to 40% for all studied samples. Resolution analysis of the PBS primers showed all of them to have high or medium polymorphism levels, which varied from 0.763 to 0.965. Results of the molecular analysis of variance showed that the general biodiversity of A. ledebourianum populations is due to interpopulation (67%) and intrapopulation (33%) differences. The revealed genetic diversity was higher in the most distant population of A. ledebourianum LD64, located on the Sarymsakty ridge of Southern Altai. This is the first genetic diversity study of the endemic species A. ledebourianum using DNA profiling approaches. This work allowed us to collect new genetic data on the structure of A. ledebourianum populations in the Altai for subsequent development of preservation strategies to enhance the reproduction of this relict species. The results will be useful for the conservation and exploitation of this species, serving as the basis for further studies of its evolution and ecology.

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Section: Discussionmentioning

confidence: 99%