Pedigree Reconstruction of the Italian Grapevine Aglianico (Vitis vinifera L.) from Campania

Abstract

A total of 41 accessions of Aglianico belonging to three different biotypes (Taburno, Taurasi, and Vulture) and 9 accessions of Sirica grapes were sampled from diverse areas of Campania (Italy). All accessions were first genotyped using 21 microsatellite markers (SSR) to evaluate possible homonymies, synonymies, and the genetic structure of each group. A larger dataset was then constructed adding Italian and International cultivars. On the basis of results obtained analyzing the first dataset, further investigations were carried out enlarging the number of investigated loci (up to 43). The addition of 22 SSRs was useful in the definition of likely genetic relationships linking Aglianico biotypes, Sirica and Syrah. According to their SSR allelic profiles, the monophyletic origin of the three Aglianico biotypes was confirmed. Among Aglianico Taburno accessions, eight samples (called Aglianico like-to-type) performed a different SSR allelic profile from Aglianico true-to-type. Sirica and Syrah proved to be synonyms. This work allowed to determine the genetic relationship between Aglianico and the cultivars supposed to be related. The parentage analysis was investigated. The most likely pedigree has been reconstructed; revealing a second-degree relationship between the worldwide cultivated Syrah from the Rhone Valley and Aglianico. Aglianico like-to-type appeared related to Aglianico in a parent-offspring fashion.

High-throughput 18K SNP array to assess genetic variability of the main grapevine cultivars from Sicily

“…DNA quality (260/230 and 260/280 ratios) and concentration was checked by NanoDrop Spectrophotometer (Thermo Scientific, Waltham, MA). Multiplexed amplification reactions were performed in 25 μl final volume reaction mixture as described in De Lorenzis et al (2013). The amplification products were solved on ABI PRISM 310 Genetic Analyser (Applied Biosystems by Life Technologies, Foster City, USA), and the alleles were sized by GENEMAPPER 4.0 (Applied Biosystems by Life Technologies).…”

Third Center of Domestication: Interaction between Genetics and Archaeological Sciences in Lands Devoted to Biodiversity of Grapevine Varieties

“…Sirica and Aglianico, the latter recognized as like-to-type and true-to-type, have a half correspondence in 37 on 43 loci (full difference in VMC2h4, VVIP60, VVIV67, VVMD32 and VVS2). They are valued 'first cousins' and the Pinot an ancestor [38][39][40][41]. Then in the transition from the culture of the 'pit Tombs' to the Greek colonisation [ * Pinot] is conceivable in use in the Apennines (the ptolemaic mesógheios) and Enotria, from Sele to Crati.…”

“…The intersections that have configured [38]. Theoretically the crossings could have happened the same time in Italy or France.…”

Evidence for a Sympatric Origin of Ribolla gialla, Gouais Blanc and Schiava cultivars (V. vinifera L.)

“…PCR amplification conditions and capillary electrophoresis detection were carried out as reported in De Lorenzis et al (2012).…”

“…Moreover, Ribolla gialla as a potential progeny of Gouais Blanc and Traminer was investigated by deep genotyping (58 SSR loci). Nuclear simple sequence repeat (SSR) markers are a useful tool to determine the genetic diversity of V. vinifera cultivars (Cipriani et al, 2010;Laucou et al, 2011;Bacilieri et al, 2013;Imazio et al, 2013), to solve cases of homonyms (This et al, 2006a(This et al, , 2006b) and synonyms, and to establish pedigree analysis (Bowers et al, 2000;Vouillamoz & Grando, 2006;Boursiquot et al, 2009;Vouillamoz & Arnold, 2010;De Lorenzis et al, 2012;Lacombe et al, 2012).…”