PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Kučerová, Jana; Svobodová, Zuzana; Knotek, Petr; Palarčík, Jiří; Vlček, M.; Kincl, M.; Horák, Daniel; Autebert, Julien; Viovy, Jean‐Louis; Bı́lková, Zuzana

doi:10.1016/j.msec.2014.04.011

Cited by 17 publications

(11 citation statements)

References 62 publications

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“…As a result, the zeta potential of the coated particle is decreased. More details on the influence of PEG on particle's electrokinetic behavior can be found in the several review papers .…”

Section: Introductionmentioning

confidence: 99%

Zeta potentials of PDMS surfaces modified with poly(ethylene glycol) by physisorption

et al. 2019

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Controlling zeta potential of PDMS surface coated with a layer of PEG is important for electroosmosis and electrophoresis in PDMS made microfluidic chips. Here, zeta potentials of PDMS surfaces modified by simple physisorption of PEG of different concentrations in phosphate buffer solutions, pure water, and PEG solution were reported. Coating PEG on PDMS surfaces was achieved by immersing a PDMS layer into the PEG solution for 10 min and then taking it out and placing it in an oven at 80 • C for 10 h. To avoid damaging the PEG layer on the PDMS surface, an induction current method was employed for zeta potential measurement. Zeta potentials of PEG modified PDMS in electrolyte solutions were measured. The results show that 2.5% PEG can effectively modify PDMS surface with positive zeta potential value in phosphate buffer solutions, pure water and 10% PEG solution. Further increase in PEG solution beyond 5% for surface modification has no obvious effect on zeta potential change.

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“…As a result, the zeta potential of the coated particle is decreased. More details on the influence of PEG on particle's electrokinetic behavior can be found in the several review papers .…”

Section: Introductionmentioning

confidence: 99%

Zeta potentials of PDMS surfaces modified with poly(ethylene glycol) by physisorption

et al. 2019

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“…2, Due to limited prolongation of AFM piezoceramics (see the limits for crystals with heights >4 µm [36]), the p(GMA-MOEAA)-NH 2 particles were analysed using SEM in a similar procedure as described previously [27]. The naked particles were smooth, and after coating by oligo-HA4 we detected no perturbations (Fig.…”

Section: Resultsmentioning

confidence: 86%

“…The topography and force spectroscopy modes of the AFM were executed using the Solver Pro M instrument (NT-MDT, St. Peterburg, Russia). Data for diameter, topography and force spectroscopy were determined according to procedures described previously [27][28][29].…”

Section: Sem and Afm Measurementmentioning

confidence: 99%

Magnetic microparticles post-synthetically coated by hyaluronic acid as an enhanced carrier for microfluidic bioanalysis

Holubová

Knotek

Palarčík

et al. 2014

Materials Science and Engineering: C

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Iron oxide based particles functionalized by bioactive molecules have been utilized extensively in biotechnology and biomedicine. Despite their already proven advantages, instability under changing reaction conditions, non-specific sorption of biomolecules on the particles' surfaces, and iron oxide leakage from the naked particles can greatly limit their application. As confirmed many times, surface treatment with an appropriate stabilizer helps to minimize these disadvantages. In this work, we describe enhanced post-synthetic surface modification of superparamagnetic microparticles varying in materials and size using hyaluronic acid (HA) in various chain lengths. Scanning electron microscopy, atomic force microscopy, phase analysis light scattering and laser diffraction are the methods used for characterization of HA-coated particles. The zeta potential and thickness of HA-layer of HA-coated Dynabeads M270 Amine were -50 mV and 85 nm, respectively, and of HA-coated p(GMA-MOEAA)-NH2 were -38 mV and 140 nm, respectively. The electrochemical analysis confirmed the zero leakage of magnetic material and no reactivity of particles with hydrogen peroxide. The rate of non-specific sorption of bovine serum albumin was reduced up to 50% of naked ones. The coating efficiency and suitability of biopolymer-based microparticles for magnetically active microfluidic devices was confirmed. Highlights: Post-synthetic surface modification of magnetic microparticles by hyaluronic acid  Hyaluronic acid -polymer of unique physicochemical and biological characteristics ABSTRACTIron oxide based particles functionalized by bioactive molecules have been utilized extensively in biotechnology and biomedicine. Despite their already proven advantages, instability under changing reaction conditions, non-specific sorption of biomolecules on the particles' surfaces, and iron oxide leakage from the naked particles can greatly limit their application. As confirmed many times, surface treatment with an appropriate stabilizer helps to minimize these disadvantages.In this work, we describe enhanced post-synthetic surface modification of superparamagnetic microparticles varying in materials and size using hyaluronic acid (HA) in various chain lengths. Scanning electron microscopy, atomic force microscopy, phase analysis light scattering and laser diffraction are the methods used for characterization of HA-coated particles. The zeta potential and thickness of HA-layer of HA-coated Dynabeads M270Amine were -50 mV and 85 nm, respectively, and of HA-coated p(GMA-MOEAA)-NH 2 were -38 mV and 140 nm, respectively. The electrochemical analysis confirmed the zero leakage of magnetic material and no reactivity of particles with hydrogen peroxide. The rate of nonspecific sorption of bovine serum albumin was reduced up to 50% of naked ones. The coating efficiency and suitability of biopolymer-based microparticles for magnetically active microfluidic devices was confirmed.

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“…A histogram was determined using optical microscopy (Olympus BX 60) after spin-coating of a water-colloidal solution onto an HCl-etched microscope slide. The diameter of the crystals was determined according to the procedure described in [19] for the number of particles > 200 µm, employing SW Gwyddion version 2.25 [20].…”

Section: Chemical Analysismentioning

confidence: 99%

4,6-Diazido-N-(2,4,6-trinitrophenyl)-1,3,5-triazin-2-amine (TNADAzT) and Its Silver Salt - Synthesis and Characterization

Musil

Matyáš

Zeman

et al. 2017

Cent. Eur. J. Energ. Mater.

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4,6-Diazido-N-(2,4,6-trinitrophenyl)-1,3,5-triazin-2-amine (TNADAzT) and its silver salt (AgTNADAzT) were prepared and characterized. Elemental analysis, FTIR, NMR, DSC, AAS and X-ray diffraction were used for analytical characterization. The sensitivities of TNADAzT and AgTNADAzT were determined and compared with common explosives and MTX-1. The crystal density of TNADAzT is 1.794 g·cm −3 and its heat of formation 899 kJ·mol −1 . The sensitivity of TNADAzT to impact and friction slightly exceeds PETN; the sensitivity to electrostatic discharge is lower than RDX. The sensitivity of AgTNADAzT is on the level of a primary explosives (between mercury fulminate and PETN). The initiation efficiency of AgTNADAzT is higher than 200 mg (acceptor PETN compressed by 64-70 MPa) and therefore excludes it from practical use as a primary explosive in detonators.

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PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

Cited by 17 publications

References 62 publications

Zeta potentials of PDMS surfaces modified with poly(ethylene glycol) by physisorption

Zeta potentials of PDMS surfaces modified with poly(ethylene glycol) by physisorption

Magnetic microparticles post-synthetically coated by hyaluronic acid as an enhanced carrier for microfluidic bioanalysis

4,6-Diazido-N-(2,4,6-trinitrophenyl)-1,3,5-triazin-2-amine (TNADAzT) and Its Silver Salt - Synthesis and Characterization

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