Pellet-freezing of Damascus goat semen in a chemically defined extender

Khalifa, T. A. A.; El-Saidy, B. E.

doi:10.1016/j.anireprosci.2005.08.008

Cited by 27 publications

(23 citation statements)

References 48 publications

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“…At the highest dilution these beneficial elements could be diluted, reducing spermatozoa protection. Similar results have been reported by [30,2,45].…”

Section: Raw Semen Quality: Data Presented Insupporting

confidence: 92%

Effect of Sperm Concentration and Site of Insemination on Conception Rate of Rabbits

Rowida¹,

Rriad²,

Tst³

et al. 2016

AJVS

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A total number of 16 mature bucks and 190 monoparous does' of New Zealand White rabbits were used in this work. The aim of this study was to evaluate some reproductive traits and semen quality. The traits measured were semen-ejaculate; sperm motility; sperm-cell concentration; the percentages of live spermatozoa; total-sperm abnormalities and acrosomal damages by conventional technique. Pooled semen sample was divided into two homogeneous parts then diluted with glucose yolk citrate diluents. The dilution rates were 1: 5 and 1: 10. The kindling rate and litter size at birth were recorded. Results of first experiment showed that the ejaculates with high percentage of motile spermatozoa was 74.42 ± 3.34; low percentage of dead spermatozoa was17.02 ± 2.71; sperm abnormalities (14.39 ± 1.03); acrosomal damages prior to their inclusion in the final pool (12.26 ± 0.96), and sperm cell concentration (450.41±43.94). Results of second experiment indicated that the average length of the vaginal tract was 14.5 ± 19.5 cm. Semen dilution rate at 1: 5 resulted high significant (P<0.05) conception and kindling rates compared to semen dilution rate of 1:10. Similarly, the conception rate was increased significantly (P<0.05), where the insemination catheter deeply deposits diluted semen into vaginal tract to depth 12 cm, giving better conception and kindling rates than in the case of 4 or 8 cm.

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“…At the highest dilution these beneficial elements could be diluted, reducing spermatozoa protection. Similar results have been reported by [30,2,45].…”

Section: Raw Semen Quality: Data Presented Insupporting

confidence: 92%

Effect of Sperm Concentration and Site of Insemination on Conception Rate of Rabbits

Rowida¹,

Rriad²,

Tst³

et al. 2016

AJVS

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“…Semen has been preserved from domesticated animals [6] using various extenders including cow's milk [7] and coconut milk. Semen was also collected from nondomesticated animals, such as hares ( Lepus europaeus ) [8].…”

Section: Introductionmentioning

confidence: 99%

Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water

Mollineau

Adogwa²,

Garcia³

2011

Veterinary Medicine International

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This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 × 106 spermatozoa/ml. Experiment 1: 200 ejaculates were extended for liquid storage at 5∘C and evaluated every day for 5 days to determine FPM% and its rate of deterioration. Experiment 2: 150 ejaculates were extended for storage as frozen pellets in liquid nitrogen at −195∘C, thawed at 30∘ to 70∘C for 20 to 50 seconds after 5 days and evaluated for FPM% and its rate of deterioration. Samples treated with UHT milk and storage at concentrations of 100 × 106 spermatozoa/ml produced the highest means for FPM% and the slowest rates of deterioration during Experiment 1. During Experiment 2 samples thawed at 30∘C for 20 seconds exhibited the highest means for FPM% (12.18 ± 1.33%), 85% rate of deterioration. However, samples were incompletely thawed. This was attributed to the diameter of the frozen pellets which was 1 cm. It was concluded that the liquid storage method was better for short term storage.

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“…These include reduced GSH, GSH-PX, catalase (CAT), superoxide dismutase (SOD), and metal chelators (transferrin, lactoferrin and ceruloplasmin) [1,7,13,15,60]. Therefore, addition of antioxidant to the extender may have positive effects on elimination of ROS [4,5,31,33,37,49,52,55]. Among different antioxidants, Butylated hydroxytoluene (BHT) is a synthetic analogue of vitamin E that engages in auto-oxidation reaction by transforming the peroxy radicals to hydroperoxides.…”

mentioning

confidence: 99%

Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze–thaw process

et al. 2013

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Pellet-freezing of Damascus goat semen in a chemically defined extender

Cited by 27 publications

References 48 publications

Effect of Sperm Concentration and Site of Insemination on Conception Rate of Rabbits

Effect of Sperm Concentration and Site of Insemination on Conception Rate of Rabbits

Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water

Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze–thaw process

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