Pemberian Ekstrak Daun Cincau (Mesona palustris BL) Meningkatkan Kadar Superoksida Dismutase (SOD) Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

Tarnajaya, Karnia; Pangkahila, Alex; Pangkahila, Wimpie; Siswanto, Ferbian Milas

doi:10.35790/jbm.10.1.2018.18995

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…Naturally the body has the ability to ward off free radicals by forming endogenous antioxidants produced by the body whose levels can be measured through GPx (Glutathione Peroxidase), catalase and Superoxide Dismutase (SOD) activity. (16) The increase of glutathione peroxidase levels in the K3 group that was given heavy physical activity and given VCO at a dose of 0.45 mL/200 g BW/day experienced a significant difference with the control group (K1) and the treatment group at a dose of 0.9 mL/200 g BW/day (K4) as in table 1. This is because Virgin Coconut Oil (VCO) contains antioxidants such as polyphenols α-tokoferol.…”

Section: Discussionmentioning

confidence: 89%

The Effect of Virgin Coconut Oil (VCO) on Malondialdehyde, Glutathione Peroxidase, and Cortisol Hormone Levels

,¹,

Pertiwi²,

Hussaana³

2023

IJMRA

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Vigorous physical activity is a physical movement that produces excessive free radicals due to leakage of electrons from the mitochondrial transport system. The body's antioxidant system is reduced due to the inability of the body's defenses to reduce the production of free radicals resulting in oxidative stress, so it requires a supply of antioxidants from outside the body such as Virgin Coconut Oil (VCO). The purpose of this study is to determine the effect of VCO administration on malondialdehyde levels, glutathione peroxidase levels and cortisol hormone levels in male Wistar rats subjected to strenuous physical activity. The research method uses an experimental with posttest only control group design. The experimental subjects were 24 male Wistar rats which were randomly divided into 4 groups (K1, K2, K3, and K4). The administration of VCO at dose of 25 mL and 50 mL are based of human weight 70 kg and the conversion dose in mice (BB = 200 g) resulted in 0.45 mL/200 g BW/day and 0.9 mL/200 g BW / day which is made into a 1 mL solution and given orally for 28 days. K3 and K4 groups were given strenuous physical activity and given VCO at a dose of 0.45 and 0.9 mL/200 g BW/day. The measurement of malondialdehyde levels using the TBARS method. The glutathione peroxidase (GPx) and the cortisol hormone using ELISA method at PSPG UGM in June 2022. The lowest average levels of MDA and GPx were in the K1 group. One-Way Anova test result showed that the levels of malondialdehyde, glutathione peroxidase, and the cortisol hormone in the K3 and K4 groups had significant differences with K2 group (p < 0.05). The administration of VCO at doses of 0.45 mL/200 g BW/day and 0.9 mL/200 g BW/day could affect the levels of malondialdehyde, glutathione peroxidase, and the hormone cortisol in male Wistar rats subjected to strenuous physical activity.

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Section: Discussionmentioning

confidence: 89%

The Effect of Virgin Coconut Oil (VCO) on Malondialdehyde, Glutathione Peroxidase, and Cortisol Hormone Levels

,¹,

Pertiwi²,

Hussaana³

2023

IJMRA

View full text Add to dashboard Cite

show abstract

“…Peroxidase is useful to prevent the accumulation of H2O2, whose existence becomes dangerous in the company of O2because it can create OH radicals, the most reactive and hazardous free radicals, which can damage cell membranes by inducing the dissolution of unsaturated fatty acids. Isoflavone content is a fat-soluble chain-breaking antioxidant that operates on cell membranes to break the lipid peroxidation chain (Tarnajaya et al, 2018).…”

Section: Effect Of Soybean Ethanol Extract On Sfit-1 Levels In Huvecs...mentioning

confidence: 99%

“…HO-1 was also found to inhibit sFlt-1 production in vitro studies. In addition, isoflavones can reduce the activation of p38 MAPK will trigger an increase in anti-angiogenic activity (Jena et al, 2020;Tarnajaya et al, 2018).…”

Section: Effect Of Soybean Ethanol Extract On Sfit-1 Levels In Huvecs...mentioning

confidence: 99%

Effect of Soybean Extract on sFlt-1 LEVELS in Huvecs Cultures Induced by Preeclampsia Plasma

Indriyani,

Mestika,

Nurseta

et al. 2023

Med.Lab.Tech.J.

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Preeclampsia is a dangerous pregnancy issue that causes hypertension at ≥20 weeks of gestation. Oxidative stress is known to play a role in the pathophysiology of preeclampsia by raising the activity of soluble fms-like tyrosine kinase 1 (sFlt-1) and causing endothelial dysfunction. As a result, antioxidants are utilized as a therapy in preeclampsia to protect the body from the impacts of free radicals. This study aims to determine the effect of soybean extract on sFlt-1 levels in HUVECs cultures exposed to preeclamptic plasma. The benefit of this study is the consideration for clinicians to provide soybean ethanol extract supplements (Glycine max) to increase the potential of antihypertensive therapy. Human umbilical vein endothelial cell cultures (HUVECs) were used in the study, which was conducted in vitro. HUVECs cultures were exposed to preeclampsia plasma and subsequently treated for 24 hours with various dosages of soybean extract. The enzyme-linked immunosorbent assay (ELISA) measured the sFlt-1 levels in each observation group. The hypothesis was tested using One-Way ANOVA analysis with SPSS version 25 software. This study found a significant difference (p <0,05) between the mean value of the positive control group and the negative control group. A significant difference was also found (p <0,05) in the mean value of sFlt-1 between the positive control group and the treatment group that was given soybean extract at doses of 17,5 ppm. Soybean extract had a significant effect on decreasing sFlt-1 levels in HUVECs cultures exposed to preeclamptic plasma. Thus, soybeans isoflavones have the potential to treat preeclampsia by reducing anti-angiogenic factors.

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“…Kondisi stres oksidatif dapat dideteksi dengan mengukur biomarker kerusakan jaringan. Salah satu indikator kerusakan jaringan adalah tingginya kadar malondialdehid (MDA) (Nyandra et al, 2018;Tarnajaya et al, 2018). Malondialdehid merupakan produk akhir dari oksidasi asam lemak tak jenuh oleh radikal bebas dan merupakan metabolit komponen sel yang dihasilkan oleh radikal bebas.…”

Section: Pendahuluanunclassified

Bisphenol A Meningkatkan Malondialdehid dan Indeks Apoptosis Hati Tikus (Rattus norvegicus) Jantan

et al. 2022

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Bisphenol-A (BPA) merupakan toksikan yang diketahui dampaknya terhadap reproductive toxicities.Namun, beberapa tahun belakangan ini, diketahui pula bahwa BPA menyebabkan stres oksidatif. Stresoksidatif merupakan salah satu faktor penyebab kerusakan organ. Penelitian ini bertujuan untuk membuktikan efek pemberian BPA oral terhadap kadar malondialdehid dan indeks apoptosis pada hatitikus. Penelitian ini menggunakan posttest only control group design. Subjek adalah 14 ekor tikus putih jantan galur Sprague dawley, umur 8-10 minggu, bobot badan berkisar 180 g, dan dalam keadaan sehat. Kelompok kontrol (P0), tujuh ekor tikus, diberikan plasebo berupa 1 mL aquadest selama 21 hari; sedangkan kelompok perlakuan (P1), tujuh ekor tikus, diberikan 400 mg/kgBB tikus BPA selama 21 hari. Hasil menunjukkan bahwa kelompok P1 memiliki kadar MDA hepatik yang lebih tinggi (3,33±0,27 nmol/mg.prot) dan berbeda nyata (p<0,001) dibandingkan kelompok P0 (2,67±0,14 nmol/mg.prot). Selain itu, kelompok P1 memiliki indeks apoptosis yang lebih tinggi (11,21±2,26%) dan berbeda nyata (p<0,001) dibandingkan kelompok P0 (2,19±0,97%). Berdasarkan hasil tersebut, dapat disimpulkan bahwa pemberian BPA oral meningkatkan malondialdehid dan indeks apoptosis pada hati tikus putih (Rattus norvegicus) jantan.

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Pemberian Ekstrak Daun Cincau (Mesona palustris BL) Meningkatkan Kadar Superoksida Dismutase (SOD) Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

Cited by 7 publications

References 11 publications

The Effect of Virgin Coconut Oil (VCO) on Malondialdehyde, Glutathione Peroxidase, and Cortisol Hormone Levels

The Effect of Virgin Coconut Oil (VCO) on Malondialdehyde, Glutathione Peroxidase, and Cortisol Hormone Levels

Effect of Soybean Extract on sFlt-1 LEVELS in Huvecs Cultures Induced by Preeclampsia Plasma

Bisphenol A Meningkatkan Malondialdehid dan Indeks Apoptosis Hati Tikus (Rattus norvegicus) Jantan

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