This study was aimed to prove that administration of black cincau (Mesona palustris BL) leaf extract increased superoxide dismutase (SOD) levels of overtraining-induced male Wistar rats (Rattus norvegicus). This was a true experimental study using the randomized pretest-posttest control group design. Subjects were 16 male rats (Rattus norvegicus), 6-month old, Wistar strain, weighing 180-200 g, SOD level <39.3 U/mL (normal SOD level), divided into two groups: the control group (P0) and the treatment group (P1). The P0 group was given excessive physical training and aquadest for 14 days, while the P1 group was given excessive physical training and black cincau leaf extract of 54 mg/200 g BW daily for 14 days. The results showed that the mean SOD level before treatment (pretest) in the P0 group was 30.63±2.26 U/mL while in the P1 group was 31.31±2.52 U/mL (P >0.05). After 14-day treatment (posttest), the mean SOD level in the P0 group was 29.36±2.83 U/mL while in the P1 group was 41.31±2.35 U/mL (P <0.01). Further analysis showed that there was no change of SOD levels in the P0 group (P > 0.05), but in the P1 group there was a significant increase of SOD levels from 31.31±2.52U/mL to 41.31±2.35U/mL (P <0.01). Conclusion: Administration of black cincau leaf extract of 54 mg per 200 g of body weight increased SOD level of overtraining-induced male Wistar rats (Rattus norvegicus).Keywords: black cincau leaves, SOD, excessive physical activityAbstrak: Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun cincau (Mesona palustris BL) dapat meningkatkan kadar superoksida dismutase (SOD) tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih. Jenis penelitian ialah eksperimental murni menggunakan randomized pretest-posttest control group design. Subjek penelitian ialah 16 ekor tikus putih jantan (Rattus norvegicus) galur Wistar berumur 6 bulan, sehat, berat badan 180-200 gr, dan kadar SOD <39,3 U/mL (kadar SOD normal), yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (P0) dan kelompok perlakuan (P1), masing-masing berjumlah 8 ekor tikus. Kelompok P0 diberikan pelatihan fisik berlebih dan akuades secara sonde selama 14 hari, dan kelompok P1 diberikan pelatihan fisik berlebih dan ekstrak daun cincau dosis 54 mg/200 gr BB tikus selama 14 hari. Hasil penelitian menunjukkan bahwa rerata kadar SOD sebelum perlakuan (pretest) pada kelompok P0 ialah 30,63±2,26 U/mL, sedangkan pada kelompok P1 ialah 31,31±2,52 U/mL (P >0,05). Setelah perlakuan selama 14 hari (posttest), rerata kadar SOD pada kelompok P0 ialah 29,36±2,83 U/mL, dan pada kelompok P1 ialah 41,31±2,35 U/mL (P <0,01). Hasil analisis efek perlakuan menunjukkan bahwa pada kelompok P0 tidak terjadi perubahan kadar SOD (P >0,05), namun pada kelompok P1 terjadi peningkatan bermakna kadar SOD dari 31,31±2,52 U/mL menjadi 41,31±2,35U/mL (P <0,01). Simpulan: Ekstrak daun cincau dosis 54 mg/200 gr BB tikus dapat meningkatkan kadar SOD tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih.Kata kunci: daun cincau, SOD, aktivitas fisik berlebih

The public is increasingly aware of the importance of exercise for the elderly because exercise is an option to reduce complaints due to setbacks and improve the health of the elderly. In fact the implementation in the field is often wrong as in Banjar Benaya which implements routine exercises only once a week with a duration less than 30 minutes, does not pay attention to nutritional needs, does not use sports clothing, and displays that are not clear. The measurement of elderly physical fitness is still relatively low. Based on these problems, improvements were made to the application of ergonomic elderly gymnastics due to improve elderly’s physical fitness and increase the bone mass.  This study used a treatment by subject design conducted in April 2017-August 2018. The target population was all elderly in Denpasar and the affordable population was determined by multistage random sampling then selected Banjaya Benaya Peguyangan Village with a sample of 20 people who met the inclusion criteria. The data collection were physical fitness and bone mass. The difference in treatment effects were analyzed using a Paired Sample t-Test with α = 0.05 for data with normal distribution and Wilcoxon test, α = 0.05 for data with abnormal distribution. Data showed that there were differences in physical fitness improvement in Period I and Period II after 8 weeks of gymnastics at 43.43% with very bad categories being bad, the difference in increase in whole body bone mass was 16.76% and leg bone mass 68.67%. Analysis of Paired Sample-t-Test physical fitness data and Wilcoxon test for bone mass data in Period I and Period II after gymnastics for 8 weeks, found that there was a significant difference (p <0.05). It can be concluded that ergonomic elderly gym can significantly improve physical fitness and increase bone mass of the elderly. It is recommended that the elderly continue to exercise with a duration of 30-45 minutes, the frequency of 3 times a week to maintain elderly’s physical fitness.

Background: Excessive sweating of the body is a reaction to decrease the heat caused by prolonged exercise at high relative humidity (RH). This situation may cause an increase in pulse rate (PR), body temperature (BT), and blood lactic acid (BLA) workout. Objective: This study aimed to prove that a RH of 40% better than a RH of 50% and 60% RH in inhibiting the increase of PR, BT, and BLA during exercise. Methods: The study was conducted on 54 samples randomly selected from the IKIP PGRI Bali students. The samples were divided into three groups, and each group was given cycling exercise with a load of 80 Watt for 2 x 30 minutes with rest between sets for five minutes. Group-1 of cycling at 40% of RH, Group-2 at a RH of 50%, and the Group-3 at a RH of 60%. Data PR, BT, and BLA taken before and during exercise.The mean difference between groups before and during exercise were analyzed by One-way Anova and a further test used Least Significant Difference (LSD). Significance used was α = 0.05. Results: The mean of PR during exercise was significantly different between groups with p = 0.045, the mean of BT during exercises was significantly different between groups with p = 0.006, and the mean of BLA during exercises was significantly different between groups with p = 0.005 (p <0.05). Also found that PR, BT, and BLA during exercise at 40% RH was lower than 50% RH and 60% RH (p <0.05). Conclusion: Thus, the RH of 40% was better than RH of 50% and 60 % in inhibiting the increase of PR, BT, and BLA during exercise. Therefore, when practiced in a closed room is expected at 40% relative humidity.Keywords: relative humidity, pulse rate, body temperature, blood lactic acid. Cite This Article: Sandi, N., Pangkahila, A., Adiatmika, P.2016. Relative humidity of 40% inhibiting the increase of pulse rate, body temperature, and blood lactic acid during exercise.

Latar belakang dan tujuan: Pemberian makanan tambahan pemulihan (PMT-P) dilakukan untuk perbaikan status gizi kurang anak balita. Tahun 2012 di Kabupaten Sumba Timur Nusa Tenggara Timur (NTT) terdapat 21% balita gizi kurang dan di wilayah kerja Puskesmas Kawangu tercatat 3,4% balita gizi kurang. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan dan motivasi kader posyandu terhadap perilaku asupan dan perubahan status balita gizi kurang di wilayah Puskesmas Kawangu, Kecamatan Pandawai, Kabupaten Sumba Timur. Metode: Desain penelitian cross-sectional dengan jumlah sampel sebanyak 80 balita dan kader yang memenuhi kriteria inklusi, serta Ibu balita sebagai responden. Pengetahuan dan motivasi diperoleh dari wawancara dengan kader sedangkan perilaku asupan melalui wawancara dengan ibu balita. Perubahan status gizi balita diukur dengan antropometri setelah pemberian PMT-P dan dibandingkan dengan data sebelum pemberian PMT-P di puskesmas. Analisis data dilakukan secara univariat dan bivariat menggunakan uji chi-square. Hasil: Sebagian besar kader berumur 26-35 tahun (41,2%), berpendidikan SD (56,2%), petani (91,2%), menjadi kader >2 tahun (53,8%), sudah mengikuti pelatihan (72,5%) dan lama mendampingi balita gizi kurang <45 hari (91,2%). Mayoritas kader berpengetahuan baik (55,0%) dan memiliki motivasi tinggi (86,2%). Perilaku asupan balita cukup (53,8%) dan peningkatan status gizi ke kategori baik (63,8%). Ada hubungan pengetahuan kader dengan perilaku asupan (p=0,016) dan tidak ada hubungan motivasi kader dengan perilaku asupan (p=0,500). Perilaku asupan terbukti berhubungan dengan perubahan status gizi balita gizi kurang (p=0,032). Simpulan: Pengetahuan kader posyandu meningkatkan perilaku asupan balita dan meningkatkan status gizi anak balita gizi kurang.

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