The population of freshwater crablets in nature has decreased due to a decrease in: the area of rice fields, discharge, and water quality. Domestication, pollution control, and catching freshwater crablets are important to maintain their sustainability. One effort to reduce the rate of exploitation is to save on the use of seeds in cultivation activities, especially by reducing mortality during transportation and initial post-transportation maintenance. This study aims to analyze the effect of adding supplements to the growing media before and after transportation on the physiological condition, survival and condition factors of freshwater crablets (P. convexa). This research was conducted from March to May 2023 at the Aquatic Animal Physiology Laboratory. This study used a completely randomized design (CRD) with one dose of the best supplement (10 ppm) and 10 replicates. The study used analysis of variance (ANOVA) test for each parameter measured least and significant difference (LSD) test with 95% confidence interval. Freshwater crablets were given four treatments, namely A: (TS-TS), B: (TS-DS), C:(DS-DS), and D: (DS-TS). The results showed that application of supplements with a dose of 10 ppm in the fermentation media before and after transportation (treatment C: DS-DS) was the best with blood glucose levels of 30.50 mg/dL and survival of 70%. The condition factor values of freshwater crablets in the four treatments tended to decrease at the end of the observation.