The objective of this case study was to evaluate good lighting levels in laying hens. The data were collected for three months at UD. Mahakarya Farm Banyuwangi. A total of 4.400 heads of layer chicken were used in this study. The lighting intensityused were 15 lux in cage A and 10 lux in cage B. The average of egg production were75.91% and 73.57% for cage A (15 lux) and cage B (10 lux), respectively. The average of egg weight were 59.37 g and 59.23 gfor cage A (15 lux) and cage B (10 lux), respectively. The results of this study presented that layer chicken aged 25 weeks produced better production efficiency values by 10 lux. At the end of production period(38 weeks) increased production efficiency by 15 lux.

Ulva sp. is green macroalgae that very potential for producing food with high nutrients content. This species is found on a coastline in intertidal zone of Indonesia’s waters. There have been several research to this species start from post-harvest handling, nutrition, probiotic, and its ulvaran, however the information of effect of temperature storage to Ulva lactuca freshness is still lack. This study was undergone to evaluate the freshness quality of Ulva lactuca by sensory changes in different temperatures short-term storage. In order to evaluate the effect of the different storage circumstances, the fresh U. lactuca was collected from its natural habitat (intertidal zone of Sepanjang Coast, Yogyakarta Indonesia). It was rinsed from debris and epiphyte, stored in transparent polyethylene bag, and were stored in 4 °C, 15-20 °C and room temperature for five days. Color, pH, ash, moisture, crude protein, chlorophyl, Total Plate Count, sensory analysis was undergone along the storage period. The sensory evaluation score of U. lactuca in 4 °C were more than 6, and better than other storages. The crude protein (U. lactuca stored in 4 °C) decreased significantly on day 4 by 5.53%, it was lower than others. The TPC of all samples varied from 147x103in 0 days of storage to 2,462.5x103 CFU/ml on the last day of storage. In summaries, sensory scores of U. lactuca are more constant and higher in 4 °C than in other storages, despite minor nutrient content deterioration.

<strong>Determination of Daily Requirement of Trash Fish Feed to Fatten The Mangrove Crab Scylla paramamosain in Bottom Net Cages.</strong> Mangrove crab fattening is part of the crab farming activities that attracts the interests of the farmers due to its relatively short period, i.e. 14–21 days per cycle. Trash fish is a natural feed for Mangrove crabs that is easily available at an affordable price. Scientific information on the daily requirement of trash fish feed to fatten the Mangrove crabs is still scarce. The accessable scientific information on the amount of daily feeding is still limited to the rearing of the crabs. Hence, the scientific study on the daily requirement of trash fish feed for crab fattening needs to be done. Probolinggo is one of the producing districts for Mangrove crabs and is a potential area for development of crab aquaculture. Therefore, Probolinggo was chosen as the site of this research. Crab samples utilized had a carapace width of 8–13 cm and a weight of 115–500 g. Crabs were reared in bottom net cages measuring 5 x 5 m2 with a density of 16 crabs/cage and the sex ratio of 1: 1. The study consisted of 4 treatments with 3 repetitions. The daily feeding percentages given were 10, 15, 20, and 25% of the body weight of the crabs. The parameters observed during the 13 days of experiment were growth, survival rate, and the percentage of already fatter crabs. The results showed that the daily feed ration of trash fish, as much as 10% dan 15% of the body weight of the crab, gave the best yield for crab fattening. The survival rate obtained was 70% of the initial density of 0.64 crabs/m2, with a total weight gain of 14% during the study. <br /><br />

DEVELOPMENT OF MUD CRAB HATCHING METHODS. Indonesia is one of the mud crab exporters. The demand of mud crab products is high, but these products are still dominated by natural catches. Although there are many cultivation efforts, the seeds are still using the catch from nature, which may lead to an unsustainable business. In order to maintain the existing mud crab cultivation business, it is necessary to undertake mass production of crab seeds in an artificial environment, such as in the hatchery or in the laboratory. The research of mud crab larvae maintenance has been carried out in Indonesia since the last four decades, but the results have not been able to produce crab seeds in a mass scale. Therefore, the efforts of hatching crabs in hatcheries must continue for improvement, with techniques and methods should be developed to support sustainable crab enlargement cultivation.

<strong>Determination of Calcium Concentration in Formulated Feed for Early Zoea Crab Scylla paramamosain.</strong> Calcification process of the new shell during postmoulting of crab fries needs calcium from food and water in the environment. However, the digestive enzymes in the early phase of zoea have not functioned perfectly yet, so the artificial feed given in the ration of crab fries may be less digestable. Therefore, in this study Enzyplex (commercial enzyme) was added to the formulated feed for rearing of crab fries (zoea at early phase). The purpose of this study was to determine the effect of calcium addition into the formulated feed on the growth and survival of mud crab Scylla paramamosain Zoea. The study was conducted in August 2015 for 3 days. Zoea were kept in ecoplast containers with a stocking density of 50 ind / L. Calcium-D Kalzana was added as much as 0 mg/g (A), 50 mg/g (B), 100 mg/g (C), 150 mg/g (D ), and 200 mg/g (E) per batter (34.50 g) of  formulated feed, with 9 replications for each treatment. All ecoplast containers were placed in a waterbath at 30°C. The results show that the addition of calcium at a dose of 50 mg/g can successfully support the growth of Zoea I into Zoea II (66%) with the highest survival rate (7.6 ± 4.98%). The size of cephalothorax (597.69 ± 65.81 m), abdomen ( 1135.39 ± 126.47 m), and telson (409.23 ± 61.80 m) are shown to be larger than the other four treatments.<br /><br />