Penetration behavior of Vibrio cholerae hemolysin into (DMPC/cholesterol) mixed monolayer

PM-IRRAS Studies of DMPC Bilayers Supported on Au(111) Electrodes Modified with Hydrophilic Monolayers of Thioglucose

“…10,11,12 Such models have been successfully used to study the incorporation of peptides or interactions with toxins. 13,14,15,16 After transferring the phospholipid bilayer onto the solid support, the head groups of the bottom layer are directly in contact with the metal surface, which influences the overall structure of the bilayer. This issue becomes especially important in studies involving the incorporation of transmembrane proteins into the supported membranes because contact with metal may lead to denaturation or changes in the functionality of the proteins.…”

“…In order to better mimic a natural biological membrane, the combination of Langmuir–Blodgett and Langmuir–Schaefer techniques may be employed to prepare supported bilayer lipid membranes (sBLMs). Typically, a single phospholipid species, such as DMPC, is used to assemble the biomimetic layers onto the gold surface. , Alternatively, cholesterol may be introduced into these films to improve the elastic properties of the membrane and more closely resemble the true composition of biological cell membranes. , In addition, the transfer of the bilayers onto the surface of the electrode enables the application of various electrochemical, microscopic, and spectroscopic techniques to gain a molecular level description of the sBLM, including the morphology, orientation, conformation, and organization of the molecules within the bilayer. − Such models have been successfully used to study the incorporation of peptides or interactions with toxins. − …”