Penetration of Singlet Oxygen into Films with Oxygen Permeability Coefficient Close to that of Skin

Abstract

Although its antiviral and antibacterial functions help prevent infection, singlet oxygen ( 1 O 2 )-which is generated by the action of light on an endogenous photosensitizer-is cytotoxic. In the present study, we investigated the ability of 1 O 2 -generated by the action of visible light on a photosensitizer-to penetrate skin. We used two polymer films with oxygen permeability coefficients similar to that of skin-i.e. cellulose acetate (CA) and ethyl cellulose (EC). Both films contained 1,3-diphenylisobenzofuran (DPBF), which was used as an 1 O 2 probe. 1 O 2 generated externally did not permeate the films by mere contact. Therefore, we conclude that the potential for 1 O 2 to penetrate the skin is very low, and films that generate 1 O 2 are safe and useful for preventing infections by contact. We also proved that 1 O 2 can move between the layers of integrated polymer films when they are joined together.

Long‐Lived Luminescence Emitted from Imide Compounds Dispersed in Polymer Matrices after Continuous Ultraviolet Irradiation and its Relation to Oxygen Quenching

“…DPBF has a characteristic absorption peak at 418 nm, whereas decomposed DBB does not. Therefore, the generation of 1 O 2 can be qualitatively monitored through the time variation of the absorption peak of DPBF [41,42] . PMMA films containing homogeneously dispersed both DPBF and ICs and those containing only DPBF were prepared to detect 1 O 2 and monitor the photodegradation of DPBF, respectively.…”

“…Therefore, the generation of 1 O 2 can be qualitatively monitored through the time variation of the absorption peak of DPBF. [41,42] PMMA films containing homogeneously dispersed both DPBF and ICs and those containing only DPBF were prepared to detect 1 O 2 and monitor the photodegradation of DPBF, respectively. Moreover, PMMA films containing β-carotene in addition to DPBF and ICs were prepared to estimate the rate of direct photodecomposition of DPBF by UV irradiation and not via the reaction with 1 O 2 .…”

Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces microbial load in rat acute peritonitis model

“…The nitrite concentration, singlet oxygen content, and overall oxidative strength of the PTS solution increased with plasma treatment time, implying that ROS and RNS play a role in the antimicrobial effect of the PTS solution. The detection of singlet oxygen in the PTS solution was carried out using the DPBF reagent, which is a yellow-colored reagent that reacts with singlet oxygen to yield a colorless product 38 , 39 . Therefore, lower absorption of the DPBF, when exposed to plasma-treated liquid, reflects a higher amount of singlet oxygen.…”

Engineering H2O2 and O2 Self‐Supplying Nanoreactor to Conduct Synergistic Chemiexcited Photodynamic and Calcium‐Overloaded Therapy in Orthotopic Hepatic Tumors

“…Extracellular Measurement of 1 O 2 Generation: 1,3-diphenylisobenzofuran (DPBF) is a popular 1 O 2 trapping-agent. [47] DPBF solution (10 μL, 3 mg mL −1 in ethanol) was added to the mSCCC or mSCCC@SA solution (100 ppm, 0.1 mL). Then, 1 mL PBS solution was added and the absorbance of DPBF solution at 410 nm was recorded at the appointed time.…”