Penetration of Zona-Free Eggs by Spermatozoa of Different Species

Hanada, Akira; Chang, M. C.

doi:10.1093/biolreprod/6.2.300

Cited by 159 publications

(57 citation statements)

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“…The fertilization of human oocytes in vitro has proved technically possible (Steptoe & (Hanada & Chang, 1972). Human spermatozoa are capable of penetrating zona-free hamster oocytes after an in-vitro incubation probably associated with capacitation (Yanagimachi, Yanagimachi & Rogers, 1976), and a relationship may occur between this in-vitro penetration and infertility (Barros, Gonzalez, Herrera & Bustos-Obregon, 1978 …”

Section: Introductionmentioning

confidence: 99%

Heterologous ovum penetration by human spermatozoa

Tyler¹,

Pryor²,

Collins³

1981

Reproduction

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Summary. An . Of the 586 ova tested with spermatozoa from the infertile subjects only 11 (1\m=.\9%) showed any evidence of penetration (range of individual penetration rates 0\p=n-\8\m=.\7%) and binding to the vitelline membrane was poor (0 or <5 spermatozoa/ ovum). Spermatozoa from a further 9 infertile men who had abnormal spermiograms also gave poor penetration rates (4/300 ova, 1\m=.\3%). It is concluded that this bioassay has a useful role as an additional test to the classic spermiogram, but that its routine use is best reserved for selected cases of unexplained infertility.

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Section: Introductionmentioning

confidence: 99%

Heterologous ovum penetration by human spermatozoa

Tyler¹,

Pryor²,

Collins³

1981

Reproduction

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“…In addition, it is also well established that a considerable degree of species specificity is retained by this step of membrane fusion in many mammals (Hanada and Chang, 1972 (Tezon et al, 1982).…”

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confidence: 99%

On the role of epididymal factors in sperm fertility

Blaquier¹,

Cameo²,

Cuasnicú³

et al. 1988

Reprod. Nutr. Dévelop.

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Notwithstanding our improved descriptive knowledge of events occurring to mammalian sperm during epididymal maturation, the nature of the mechanisms involved in the development of such primary functions as forward motility (Bedford, 1975) and zona pellucida recognition (Saling, 1982) remain obscure.This review attempts to summarize the latest information produced by our laboratory in relation to the interaction of epididymal products with spermatozoa and the bearing that this interaction might have on sperm function.Our interest has been primarily focused on androgen-induced secretory epididymal proteins that associate to sperm during epididymal transit and that our group has described in rats (Cameo and Blaquier, 1976), hamsters (Gonzalez Echeverria, Cuasnic6 and Blaquier, 1982) and humans (Tezón et al., 1985b).Immature hamster sperm, obtained from the proximal segments of the epididymis, were used as a model to induce in vitro modifications of their functions (Cuasnicu et al., 1984a). Exposure of these spermatozoa to a preparation enriched with androgen-induced secretory proteins EP2-EP3 (Gonzalez Echeverria, was able to induce a significant increase in homologous zona pellucida binding (Cuasnicu et al., 1984b). Since zona binding seemed to be one of the functions developed by sperm during epididymal transit (Cuasnic6 et al., 1984a;Saling, 1982), our results were interpreted as reflecting the in vitro advancement of the maturational stage of these spermatozoa which was caused by the added epididymal preparation. Similarly, we were also able to show that immature sperm gained in fertilizing ability when pre-treated in vitro with the epididymal extract prior to in vivo or in vitro insemination (Gonzalez Echeverria et al., 1984).Our latest results confirm these preliminary findings and support the role of epididymal proteins in sperm maturation. Briefly, our studies show that the preparation of the epididymal proteins mentioned above, in which EP2-EP3 represented 16 % of the total protein, had a biological activity such that, on the average, treated sperm fertilized twice as many oocytes as their untreated controls (Gonzalez Echeverria et al., 1984 (Gwatkin, 1977)

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“…Rodent/non-rodents interspecies assays by IVF and ICSI have been used to evaluate sperm capacitation status (e.g. sperm penetration assay into zona-free hamster oocytes; Hanada & Chang, 1972) and sperm-borne oocyte activating factor (SOAF) (e.g. mouse oocyte activation assay; Rybouchkin et al, 1995), respectively.…”

Section: Discussionmentioning

confidence: 99%

Contribution of spermatozoal centrosomes to the microtubule-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)

Kobayashi

Amemiya²,

Takeuchi³

et al. 2006

Zygote

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SummaryUsing an interspecies microinsemination assay with bovine oocytes, it was examined whether centrosomes of Antarctic minke whale spermatozoa function as the microtubule-organizing centre (MTOC). Bull and rat spermatozoa were used as positive and negative controls, respectively. Vitrified-warmed bovine mature oocytes were subjected to immunostaining against α-tubulin 4-6 h after intracytoplasmic injection (ICSI) of 5 mM dithiothreitol-treated spermatozoa. Aster formation occurred from whale spermatozoa (33%) and bull spermatozoa (33%), but very little from rat spermatozoa (3%). Activation treatment for the microinseminated oocytes with 7% ethanol + 2 mM 6-dimethylaminopurine resulted in a similar proportion of oocytes forming a whale sperm aster (35% vs 27% in the non-treated group; 4 h after ICSI) but a significantly larger aster (ratio of aster diameter to oocyte diameter, 0.57 vs 0.30 in the non-treated group). These results indicate that the centrosome introduced into bovine oocytes by whale spermatozoa contributes to the MTOC and that assembly of the microtubule network is promoted by oocyte activation.

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Penetration of Zona-Free Eggs by Spermatozoa of Different Species

Cited by 159 publications

References 0 publications

Heterologous ovum penetration by human spermatozoa

Heterologous ovum penetration by human spermatozoa

On the role of epididymal factors in sperm fertility

Contribution of spermatozoal centrosomes to the microtubule-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)

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