Penetration rate of glutaraldehyde in various buffers into plant tissue and gelatin gels

Coetzee, J. N.; Merwe, C.F. van der

doi:10.1111/j.1365-2818.1985.tb02570.x

Cited by 29 publications

(10 citation statements)

References 25 publications

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“…Based on our results we would recommend pressure fixation of in situ vessels be carried out for 10-15 min using 2% glutaraldehyde. Coetzee and Van der Merwe [40] showed that the gel time for bovine serum albumin changed little, when glutaraldehyde concentrations above 3% were used; however, the gel time increased rapidly at concentrations below 2%. This suggests that vessels fixed in gluta raldehyde at less than 2% concentration would require a longer fixation time.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Measurement of Fixation Rate and Dimension Changes in the Aldehyde/Pressure-Fixed Canine Carotid Artery

Kratky

Lo²,

Roach³

1991

J Vasc Res

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Excised canine carotid arteries held at constant physiologic length were cycled from –13 to +27 kPa with a constant-flow infusion pump, and the pressure-volume curves were recorded. The change in diameter on reducing the pressure from 16 to 0 kPa was determined and the strain of recoil [(diameter₁₆ – diameter₀)/diameter₁₆] calculated. Diameter recoil was reduced from 51 % (fresh tissue) to 5 % after 3 h pressure fixation in 4 % formaldehyde and to 9% after 15 min in 2% glutaraldehyde with little further change. Lengthwise recoil was reduced from 37 to 10% after 5 min in 2% glutaraldehyde. Photographs were taken to measure outer diameter during pressure fixation. There was no change in diameter in either fixative from the first minute up to 3 days at the constant pressure of 16 kPa.

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Section: Discussionmentioning

confidence: 99%

Quantitative Measurement of Fixation Rate and Dimension Changes in the Aldehyde/Pressure-Fixed Canine Carotid Artery

Kratky

Lo²,

Roach³

1991

J Vasc Res

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“…However, it is highly unlikely that labile structures such as protuberances could be preserved by any such traditional chemical ®xation procedure. Signi®cant alteration of cellular structures is now considered implicit with any such protocol that requires seconds or minutes to immobilize intracellular components (Coetzee and Van der Merwe 1985;Mersey and McCully 1978). Undoubtedly, the Weier and Thomson (1962) structures that were purported as protuberances (Kohler et al 1997a), represent artifact.…”

Section: Introductionmentioning

confidence: 98%

Ultrastructure of chloroplast protuberances in rice leaves preserved by high-pressure freezing

Bourett

Czymmek

Howard

1999

Planta

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High-pressure freezing and freeze substitution were used to prepare leaves of rice (Oryza sativa L.) for ultrastructural analysis. Under these preparative conditions, plastid-derived stroma-containing protuberances were preserved and described with the electron microscope for the ®rst time. Similar protuberances were observed previously only in living cells examined with the light microscope. Infoldings of the chloroplast inner envelope were a prominent ultrastructural feature of protuberances. Infoldings were also observed in the main body of the chloroplasts and sometimes appeared contiguous with thylakoid membranes. Protuberances also contained infoldings in the form of bifurcated tubules. Apparent interconnection between protuberances of adjacent plastids was observed only in one instance. A distinct gradient in the staining density of thylakoid lumina appeared to be a function of grana position and orientation relative to the cell wall. Immunocytochemistry was used to determine that the stroma within protuberances contained 1,5-bisphosphate carboxylase/oxygenase enzyme.

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“…In plant tissues, the presence of large vacuoles, intercellular gas (Roland 1978), and especially polysaccharide cell walls contributes to further slow down the fixative flow (Coetzee and van der Merwe 1985). Furthermore, fixative efficiency is minimized by the acidic conditions encountered in most plant cell types.…”

Section: Discussionmentioning

confidence: 99%

In situ fixation of grape berries

Diakou

Carde

2001

Protoplasma

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Usual immersion protocols in aldehyde solutions fail to fully preserve the fine structure of both exocarp and mesocarp cells of grape berries, especially for the veraison (onset of ripening) and post-veraison stages. In exocarp cells, fixative diffusion is hampered by the thick polysaccharide cell walls. In mesocarp cells, plasma membrane and tonoplast are disrupted before aldehyde cross-linking occurs, owing to the high osmotic pressure and cell wall texture. The fixative was therefore injected under pressure as small droplets in the outer and inner parts of the fruit, with limited changes in the steady-state organization of fruit tissues. Compared to a selective range of immersion protocols, a striking improvement in cell preservation was observed for all berry tissues, allowing new information on various compartments of grape berry cells. The preservation of organ integrity and local concentration of aldehyde molecules are the most critical parameters of improved fixation. This technique may be applicable to a large array of fleshy fruits containing mainly cells comprising a high volumetric proportion of vacuoles accumulating large amounts of organic acids and sugars and bounded by thick-walled exocarp cells.

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Penetration rate of glutaraldehyde in various buffers into plant tissue and gelatin gels

Cited by 29 publications

References 25 publications

Quantitative Measurement of Fixation Rate and Dimension Changes in the Aldehyde/Pressure-Fixed Canine Carotid Artery

Quantitative Measurement of Fixation Rate and Dimension Changes in the Aldehyde/Pressure-Fixed Canine Carotid Artery

Ultrastructure of chloroplast protuberances in rice leaves preserved by high-pressure freezing

In situ fixation of grape berries

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