Business will be sustainable if there is a synergistic interaction among the economy’s production, economic, environmental, and social sectors. This research aims to assess the sustainability status of a hybrid grouper nursery in Situbondo, East Java. Sustainability consists of human resources, production, market, economy, technology, and institutional aspects. Multidimensional scaling (MDS) and leverage analysis were done on the data using the RAPFISH software. The average index obtained by all hybrid grouper nurseries with very sustainable status was obtained in the economic aspect with an average value of 83.05, and less sustainable status was obtained in the technological aspect at 49.71. Hybrid grouper nurseries obtained an average index ranging from 50.01 to 66.41 with a fairly sustainable status. Sixteen sensitive factors influence the sustainability index of hybrid grouper nurseries. These factors include health, social relationships between members, coaching and training, availability of transportation, road conditions, survival rate, seed prices, and technology availability. Apart from that, there are payment systems between group businesses, group help, exchange systems between businesses, profit-sharing systems between businesses, personal help, counseling, training, and sources of capital.