Pengaruh Nutrisi pada Produksi dan Karakterisasi Protease dari Bakteri Termofilik Isolat LS-1 Lumpur Sidoarjo

Poernomo, Achmad Toto; Isnaeni, Isnaeni; Agus, Djoko; Dewi, Ayu Chandra; Suryagama, Digdo

doi:10.20473/jfiki.v4i22017.51-58

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Previous research conducted by Soeka and Suliastini was isolating microbes from nature to produce proteases by fermentation with growth media without inducers. The protease activity was 315.88 U / mL, with a two-day fermentation time (Poernomo et al, 2017;Soeka & Sulistiani, 2017). The enzyme activities were tested using the supernatant metabolites of Candida tropicalis isolates, namely protease and lipase enzymes.…”

Section: Discussionmentioning

confidence: 99%

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Puspadewi

Adirestuti

Dewi

et al. 2020

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Soil microorganisms have one function as a source of producing extracellular enzymes, especially the lipase and protease enzymes used to catalysis a chemical process. Protease is one of the enzymes with the best prospects for development because it is quite widely applied in various industries, both food and non-food. Most of the lipases used in the food and pharmaceutical industries come from microorganisms. This study aimed to look at the extracellular enzyme activity of Candida tropicalis isolated from the soil of the medicinal plantations of the Faculty of Pharmacy Unjani. The study was conducted through a fermentation process to obtain bioactive metabolites of isolates. The result was analyzed using ANOVA within a 5% interval of confidence, continuing with PostHoc. Based on these results, the Candida tropicalis metabolite giving the best proteolytic index value (0,6556 ± 0,0090) U/mL. The results showed that the metabolite isolate of Candida tropicalis had the highest activity, amounting to 5,776 ± 0,495 U/mL. The best results of lipolytic index value (0,394 ± 0,053) U/mL. The Candida tropicalis metabolites produced the highest lipase enzyme after nine days of fermentation in 5.2917±0,0167 U/mL.

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Section: Discussionmentioning

confidence: 99%

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Puspadewi

Adirestuti

Dewi

et al. 2020

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“…Bacterial populations can result in high enzyme activity. Enzyme activity is strongly influenced by the nutrient content of the substrate (Poernomo et al 2017;Pazla et al 2020).…”

Section: Phytase Enzyme Activitymentioning

confidence: 99%

Degradation of phytic acid from tithonia (Tithonia diversifolia) leaves using Lactobacillus bulgaricus at different fermentation times

et al. 2021

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Abstract. Pazla R, Yanti G, Jamarun N, Arief, Elihasridas, Sucitra LS. 2021. Degradation of phytic acid from tithonia (Tithonia diversifolia) leaves using Lactobacillus bulgaricus at different fermentation times. Biodiversitas 22: 4794-4798. The aim of this study is to reduce high level of phytic acid in tithonia (Tithonia diversifolia) leaves by fermentation technique using Lactobacillus bulgaricus. A completely randomized design consisting of four treatments (fermentation time) i.e. T2: 2 days, T3: 3 days, T4: 4 days and T5: 5 days and experiment was performed in four replicates. The parameters measured were pH, phytase enzyme activity, total bacterial colony, phytic acid content, and phytic acid degradation. The results showed that the fermentation time had a significant effect (P<0.05) on pH, phytase enzyme activity, total bacterial colony, phytic acid content, and phytic acid degradation. The conclusion of this study is that on fifth day (Treatment T5) fermented tithonia leaves produced the lowest levels of phytic acid (3.48 mg/ 100g) with the highest level of degradation (64.81%).

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“…The use of plants as a source of protease is limited by the availability of arable land, suitable growth conditions, and requires a long time to produce enzymes. Protease production from animals is also limited by the availability of enzyme-producing animals (Poernomo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Protease Production from Bacillus sp. Isolated from Gastrointestinal Tract of Catfish (Clarias sp.) with Different Medium

Prihanto

Aninta

Trisnaningrum

2021

JAFH

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The need for protease in the industrial field has been increasing. Candidates for producing these enzymes can be isolated from the digestive tract of catfish (Clarias sp.). The purpose of this study was to obtain bacterial isolates that produce proteolytic from the gastrointestinal tract of catfish and determine the effect of different production media on the activity of proteolytic. The first step of this study was isolation, screening, and identification of bacteria. The second step was to test the effect of the media Luria Bertani, trypticase soy broth, and skim milk broth on proteolytic activity. Nineteen isolates were obtained from the screening process of proteolytic bacteria. Isolate no 1, was known as the best isolate in producing enzymes and was known as Bacillus sp. Tests with different growth media gave results that semi-quantitative, nutrient growth media produced the highest activity with a proteolytic index value of 2.09 ± 0.41. In addition, based on quantitative tests, the media Luria Bertani Broth produced the highest specific activity with a value of 36.479 U/mg. The conclusion of this study, Bacillus sp. from the gastrointestinal tract of catfish that cultured on the Luria Bertani Broth medium produced the best activity.

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Pengaruh Nutrisi pada Produksi dan Karakterisasi Protease dari Bakteri Termofilik Isolat LS-1 Lumpur Sidoarjo

Cited by 3 publications

References 19 publications

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Degradation of phytic acid from tithonia (Tithonia diversifolia) leaves using Lactobacillus bulgaricus at different fermentation times

Protease Production from Bacillus sp. Isolated from Gastrointestinal Tract of Catfish (Clarias sp.) with Different Medium

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