Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas

octavia, Arinda; Utomo, Budi; Madyawati, Sri Pantja; Suprayogi, Tri Wahyu; Sunarso, Agus; Hamid, Iwan Sahrial

doi:10.20473/ovz.v10i3.2021.65-71

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Flavonoids protect the plasma membrane from ROS, maintaining the integrity of the membrane and allowing for proper ATP formation. ATP is a source of energy for spermatozoa, and with the maintenance of ATP formation, sperm viability remains high (Utomo et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate

Rahmatullah,

Sugihartuti,

Susilowati

et al. 2024

MKH

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This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the motility, viability, and concentration of spermatozoa in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (CMC-Na 0.5%), C+ (4 mg/g BW of MSG), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg BW of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The results showed no significant differences in the motility and concentration of spermatozoa between the C- (81.50 ± 4.18; 7.21 ± 0.37) and T3 (77 ± 2.74; 7.04 ± 0.13) groups (p < 0.05). In addition, the results showed significant differences in the viability of spermatozoa between the C- (83.30 ± 3.40) and T3 (77.20 ± 4.06) groups. Therefore, it can be concluded that the administration of Dayak onion extracts orally to mice induced with MSG can maintain the motility, viability, and concentration of spermatozoa.

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Section: Resultsmentioning

confidence: 99%

Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate

Rahmatullah,

Sugihartuti,

Susilowati

et al. 2024

MKH

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“…Ximanes et al (2022) menyatakan bahwa penurunan persentase viabilitas terjadi karena aktivitas metabolisme spermatozoa yang menghasilkan ROS. Dipertegas oleh Oktaviani et al (2021) bahwa ROS dapat merusak membran sel spermatozoa yang menganggu proses Na +, K + dan proses Ca 2+ . Wagner et al (2017) menyatakan terganggunya permeabilitas membran plasma sel spermatozoa yang menyebabkan sumber nutrisi spermatozoa berkurang.…”

Section: Pembahasanunclassified

Penambahan Filtrat Kelopak Rosella (Hibiscus Sabdariffa) dalam Pengencer Tris Kuning Telur pada Semen Beku Kambing Kaligesing

Fuadatin,

Hariani

2024

LenteraBio

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Pelestarian genetik kambing unggul dapat dilakukan dengan penyimpanan spermatozoa pada suhu -196℃. Kualitas spermatozoa kambing dipertahankan melalui penambahan antioksidan dalam media pengencernya. Tujuan dari penelitian untuk mengetahui pengaruh penambahan filtrat kelopak rosella (FR) dalam pengencer tris kuning telur (TKT) terhadap kualitas spermatozoa kambing Kaligesing. Jenis penelitian ekperimental yang menggunakan rancangan acak lengkap (RAL) terdapat 4 perlakuan (FR 0,0%, 1,5%, 3,0% dan 4,5%) dengan 5 ulangan. Parameter yang diamati meliputi motilitas, viabilitas dan integritas membran sel spermatozoa. Analisis data secara statistik menggunakan uji Anova satu arah dilanjutkan dengan uji Duncan. Hasil uji Anova menunjukkan penambahan filtrat kelopak dalam pengencer TKT berpengaruh signifikan terhadap kualitas spermatozoa kambing Kaligesing (P<0,05). Penambahan filtrat FR 3% merupakan konsentrasi terbaik yang dapat mempertahankan kualitas spermatozoa kambing Kaligesing pada suhu penyimpanan -196℃ dengan rata-rata nilai motilitas sperma 40,10±4,93%, viabilitas 57,82±5,71%, dan integritas membran sel spermatozoa 51,78±5,15% dibandingkan dengan perlakuan lainya.

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Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke

Silviani¹,

Sukmanadi²,

Kurnijasanti³

et al. 2022

Ovz

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Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.

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Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas

Cited by 5 publications

References 19 publications

Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate

Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate

Penambahan Filtrat Kelopak Rosella (Hibiscus Sabdariffa) dalam Pengencer Tris Kuning Telur pada Semen Beku Kambing Kaligesing

Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke

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