Probiotik adalah segala bentuk sediaan sel mikroba atau komponen sel mikroba yang mempunyai pengaruh menguntungkan bagi kesehatan dan kehidupan inangnya. Beberapa aditif pakan seperti hormon Antibiotik Growth Promoter (AGP) telah dilarang. Oleh karena itu, saat ini probiotik sering digunakan sebagai bahan tambahan pengganti antibiotik. Probiotik yang banyak digunakan adalah bakteri asam laktat karena dapat meningkatkan efisiensi pencernaan dan penyerapan nutrisi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian probiotik kombinasi Bacillus coagulans dan Bacillus subtilis dengan dosis 2 ml, 4 ml, dan 6 ml/ekor/hari secara oral terhadap pertambahan bobot badan, konsumsi pakan, dan penurunan konversi pakan. ayam petelur. Penelitian ini dilakukan selama 2 minggu dengan menggunakan ayam pre layer umur 21 minggu sampai 23 minggu sebanyak 24 ekor, diacak menjadi 4 perlakuan dengan 6 ulangan dengan P0 sebagai kontrol, P1 = 2ml, P2 = 4 ml, dan P3 = 6 ml . Probiotik yang digunakan adalah probiotik kombinasi Bacillus coagulans dan Bacillus subtilis dengan konsentrasi 1x107 CFU/ml dalam bentuk cair. Kesimpulannya adalah pemberian oral kombinasi probiotik dengan Bacillus subtilis dan Bacillus coagulans dapat meningkatkan bobot badan, konsumsi pakan, dan menurunkan nilai konversi pakan ayam petelur dengan rendemen tertinggi bila diberikan dosis 4 ml.
The purpose of this research is to find the effectiveness of bandotan’s (Ageratum conyzoides L.) leaf extract on mortality of Boophilus microplus in vitro and also to get of lethal concentration and lethal time. This research used 125 Boophilus microplus. This research used five treatments, namely neguvon suspensionon (K+), ethanol 1% (K-), 1% concentration (P1), 2% concentration (P2) and 3% concentration (P3). The observation of mortality was done every six hours. Boophilus microplus dies when there is no movement. The data were analyzed using one-way ANOVA and followed by Duncan's Multiple Distance Test. The result is said to be significant if p<0.05. Probit analysis of LC50 is 1.63% at the first hour, 0.85% at the second hour, 0.54% at the third hour, 0.34% at the fourth hour, 0.04% at the fifth hour and 0.04% at the sixth hour, LC90 reached by using 5.6%, 2.9%, 1.8%, 1.2%, 0.16 and 0.16% concentration in 1, 2, 3, 4, 5 and 6 hours, respectively. The LT50 of ethanol 1% (K-) was at 10.142 hours, neguvon suspension on (K+) was at 0.747 hours, 1% concentration (P1) was at 2.184 hours, 2% concentration (P2) was at 1.579 hours, 3% concentration (P3) was at 0.923 hours and LT90 was at 23.734, 1.749, 5.110, 3.695 and 2.159 hours. The conclusion of this study was bandotan’s (Ageratum conyzoides L.) leaf extract was effective as an acaricide based on the mortality of Boophilus microplus.
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