Pengaruh Penambahan Biotin Pada Media Pertumbuhan Terhadap Produksi Sel Aspergillus niger

Wuryanti, Wuryanti

doi:10.14710/bioma.10.2.46-50

Cited by 6 publications

(5 citation statements)

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“…Oleh karena itu diperlukan tambahan nutrisi dari media sintetik seperti media berbahan dasar telur untuk mendukung pertumbuhan BTA. (Wuryanti, 2008).…”

Section: Pembahasanunclassified

Sputum Sample Quality and Delay of TCM Examination and Microscopic Results from Tuberculosis Suspect Patient

Silviani,

Prian Nirwana,

Wahyudi

2023

medankes

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Tuberculosis (TB) is a disease with a mortality rate equivalent to 11 deaths/hour caused by Mycobacterium tuberculosis. Technological developments have been able to detect TB with the GeneXpert® MTB/RIF TCM (Rapid Molecular Test) examination. The aim of this study was to determine the effect of sputum sample quality and examination delays on TCM and microscopic results in patients suspected of having tuberculosis. The research design used in this study used experimental analytics with a cross-sectional approach. The sample in this study was 40 samples taken using quota sampling. Samples were examined using Microscopic and TCM methods. In the microscopic method, 3 treatment delays are carried out, namely immediately, 5 hours and 24 hours. The results showed that all samples experienced a decrease in the number of BTA + but a decrease in grade did not occur in all samples. The decrease in consistency began to appear at a delay of 5 hours. A decrease in BTA microscopic grading often occurs when the delay is from 5 hours to 24 hours. From the results of the Friedman test, a value of α= 0.000 (< 0.05) was obtained so that it can be concluded that there is no difference in the results of microscopic examination with TCM, but there is an influence of sputum quality on BTA results (+) and there is an influence of delay time on microscopic examination of BTA.

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“…Oleh karena itu diperlukan tambahan nutrisi dari media sintetik seperti media berbahan dasar telur untuk mendukung pertumbuhan BTA. (Wuryanti, 2008).…”

Section: Pembahasanunclassified

Sputum Sample Quality and Delay of TCM Examination and Microscopic Results from Tuberculosis Suspect Patient

Silviani,

Prian Nirwana,

Wahyudi

2023

medankes

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“…Proses isolasi senyawa murni dimulai dengan pembuatan starter kultur untuk kultivasi jamur di dalam media padat yaitu SDA, kemudian diinkubasi pada suhu kamar selama 3 minggu. Pembuatan starter kultur ini bertujuan untuk memperoleh spora yang masih muda untuk menghasilkan senyawa metabolit yang lebih baik, sehingga pada tahap ini diharapkan memperoleh jamur dengan metabolit yang aktif 21 . Dari proses pembiakan jamur, koloni jamur A. niger menunjukkan adanya hifa berwarna abuabu dan spora berwarna hitam (Gambar 1).…”

Section: Hasilunclassified

Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest

Alen

Melati²,

Sarina³

et al. 2018

IJPST

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Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.Key words: isolation, A. niger, In-Habiting, M. gilvus Hagen.

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“…Kalsium yang berikatan dengan asam oksalat menjadi kalsium oksalat yang menyebabkan rendahnya penyerapan kalsium yang berimbas pada penurunan retensi kalsium yang juga terjadi pada perlakuan T3 dan T4. Menurut Wuryanti (2008) bahwa Aspergillus niger merupakan kapang yang dapat digunakan untuk menghasilkan berbagai jenis asam seperti asam oksalat. Asam oksalat dan kalsium dapat membentuk kalsium oksalat yang tidak larut sehingga menghambat penyerapan kalsium, akhirnya berakibat pada menurunkan retensi kalsium (Almatsier, 2004).…”

Section: Hasil Dan Pembahasanunclassified

Pemberian Tepung Daun Ubi Jalar Fermentasi Terhadap Massa Kalsium Dan Protein Daging Pada Ayam Kampung Super

Lutfitiana

Mahfudz

2018

Jurnal Pengembangan Penyuluhan Pertanian

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Penelitian ini bertujuan untuk mengkaji pengaruh pemberian tepung daun ubi jalar fermentasi terhadap massa kalsium dan protein daging yang berdampak pada pertumbuhan ayam kampung super. Ternak yang digunakan dalam penelitian adalah ayam kampung super umur 5 minggu sebanyak 150 ekor (unsexed) dengan bobot badan awal 441,16 + 32,57 g. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Perlakuan yang diterapkan yaitu T0 : ransum tanpa pemberian tepung daun ubi jalar, T1 : ransum dengan tepung daun ubi jalar tanpa fermentasi 10%, T2 : ransum dengan tepung daun ubi jalar fermentasi 10%, T3 : ransum dengan tepung daun ubi jalar fermentasi 13%, T4 : ransum dengan tepung daun ubi jalar fermentasi 16%. Parameter yang diamati adalah asupan protein, retensi kalsium, massa kalsium dan protein daging. Data dianalisis ragam dengan uji F dilanjutkan dengan uji Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian 10% tepung daun ubi jalar tanpa fermentasi (T1) nyata (P<0,05) menurunkan asupan protein, retensi kalsium, massa kalsium dan protein daging pada ayam kampung super. Sebaliknya, pemberian 10% tepung daun ubi jalar fermentasi (T2) menghasilkan asupan protein, massa kalsium dan protein daging sama dengan ransum tanpa tepung daun ubi jalar (T0). Berdasarkan hasil penelit ian dapatdisimpulkan bahwa pemberian tepung daun ubi jalar fermentasi 10% dapat menghasilkan massa kalsium dan protein daging yang lebih tinggi, dibandingkan dengan level pemberian tepung daun ubi jalar fermentasi lainnya.

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Pengaruh Penambahan Biotin Pada Media Pertumbuhan Terhadap Produksi Sel Aspergillus niger

Cited by 6 publications

References 0 publications

Sputum Sample Quality and Delay of TCM Examination and Microscopic Results from Tuberculosis Suspect Patient

Sputum Sample Quality and Delay of TCM Examination and Microscopic Results from Tuberculosis Suspect Patient

Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest

Pemberian Tepung Daun Ubi Jalar Fermentasi Terhadap Massa Kalsium Dan Protein Daging Pada Ayam Kampung Super

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